Microbes induced biofabrication of nanoparticles: a review

Golhani, D.K.; Khare, Ayush; Krishna, B. Gopal; Jain, Vikas; Mokka, Jagannadha Rao

doi:10.1080/24701556.2020.1731539

Cited by 11 publications

(2 citation statements)

References 167 publications

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“…Together, these results suggest that C. utilis WSH02-08 harbor at least two gold-related biotransformation pathways to transport and deposit Au(III) as Au nanoparticles (Figure S19): (1) adsorption of Au(III) on the cell wall through electrostatic interaction followed by reduction to Au nanoparticles by proteins/enzyme present on the periplasm, and (2) uptake of Au(III) into the cytoplasm and then reduction by protein/enzyme to form Au nanoparticles. ,, Meanwhile, AuNPs were observed in mitochondria and vesicles. At this point, we do not have sufficient data to determine the exact bioreductants and Au(III) reduction space in the cytoplasm.…”

Section: Resultsmentioning

confidence: 91%

Direct Visualization and Restoration of Metallic Ion-Induced Subcellular Ultrastructural Remodeling

et al. 2023

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Analysis of cellular ultrastructure dynamics and metal ions’ fate can provide insights into the interaction between living organisms and metal ions. Here, we directly visualize the distribution of biogenic metallic aggregates, ion-induced subcellular reorganization, and the corresponding regulation effect in yeast by the near-native 3D imaging approach, cryo-soft X-ray tomography (cryo-SXT). By comparative 3D morphometric assessment, we observe the gold ions disrupting cellular organelle homeostasis, resulting in noticeable distortion and folding of vacuoles, apparent fragmentation of mitochondria, extreme swelling of lipid droplets, and formation of vesicles. The reconstructed 3D architecture of treated yeast demonstrates ∼65% of Au-rich sites in the periplasm, a comprehensive quantitative assessment unobtained by TEM. We also observe some AuNPs in rarely identified subcellular sites, namely, mitochondria and vesicles. Interestingly, the amount of gold deposition is positively correlated with the volume of lipid droplets. Shifting the external starting pH to near-neutral results in the reversion of changes in organelle architectures, boosting the amount of biogenic Au nanoparticles, and increasing cell viability. This study provides a strategy to analyze the metal ions–living organism interaction from subcellular architecture and spatial localization perspectives.

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Section: Resultsmentioning

confidence: 91%

Direct Visualization and Restoration of Metallic Ion-Induced Subcellular Ultrastructural Remodeling

et al. 2023

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“…However, Tables 1–3 show us that almost all the nanoparticles are formed by the synergistic effect of several biomolecules or metabolites present in the microbial extracts. 208 It can be noted that proteins, enzymes, and certain electron donors, such as NADH, present in the bacterial extracts are responsible for nanoparticle formation. 209 Likewise, complex functional groups as well as proteins, amino acids, and enzymes in the fungal extract enable nanoparticle formation, 210 whereas polysaccharides, pigments and certain functional groups in algal extracts can lead to nanoparticle production.…”

Section: Microbial Synthesismentioning

confidence: 99%