Microbes at work in perfumery: the microbial community of vetiver root and its involvement in essential oil biogenesis

Alifano, Pietro; Giudice, Luigi Del; Talà, Adelfia; Stefano, Mario De; Maffei, Massimo E.

doi:10.1002/ffj.1978

Cited by 15 publications

(10 citation statements)

References 10 publications

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“…Biotechnological interventions including fermentation and microbial production of fragrant compounds are already being actively explored in the flavor and fragrance industry 1 2 3 . There are instances of plant-microbial associations in nature that result in formation of fragrant compounds, as in case of vetiver 4 and agarwood. In case of agarwood ( Aquilaria spp.…”

mentioning

confidence: 99%

Chemometric analysis reveals links in the formation of fragrant bio-molecules during agarwood (Aquilaria malaccensis) and fungal interactions

Sen

Dehingia

Talukdar

et al. 2017

Sci Rep

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Fragrant agarwood, arguably the costliest wood in the world, is formed by plant-fungal interactions in Aquilaria spp. However, very little is known about this fragrant outcome of interaction. Therefore, mimicking the ancient traditions of agarwood production in Assam (Northeast India), a chemometric assessment of the agarwood-fungus interaction was made by chemical profiling (GC-MS) coupled with statistical analysis (principal component, correlation network analysis) across three platforms, viz. callus, juvenile plants and resinous wood-chips with an associated Fusarium. In the study of callus-fungus interaction, increased accumulation of key aroma compounds such as pentatriacontane {fold change (log2FC) = 3.47)}, 17-pentatriacontene (log2FC = 2.95), tetradecane, 2-methyl- (log2FC = 1.10) over callus and activation of pathways related to defense and secondary metabolism indicated links to aroma production. Study on fungal interactions in juvenile plants and resinous wood-chips indicated formation of terpenoid precursors (e.g. farnesol, geranylgeraniol acetate) and agarwood sesquiterpenes (e.g. agarospirol, γ-eudesmol). Correlation network analysis revealed the possible regulation of sesquiterpene biosynthesis involving squalene. Also a direct role of fungus in aroma (e.g. dodecane, 4-methyl-, tetracosane) was highlighted. Appearance of fragrant molecules unknown to agarwood during interaction featured as a new possibility for future research.

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mentioning

confidence: 99%

Chemometric analysis reveals links in the formation of fragrant bio-molecules during agarwood (Aquilaria malaccensis) and fungal interactions

Sen

Dehingia

Talukdar

et al. 2017

Sci Rep

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“…7 ) is present in the larval osmeterial secretions produced by disturbed Pachliopta aristolochiae individuals in order to stave off numerous predators [ 99 ]. Numerous published studies have demonstrated the role of bacteria in plant terpene and terpenoid metabolism [ 100 – 105 ]. Although we only focused on cultivable gut bacteria, we hypothesize that these new volatile organic compounds, possibly derived from plant terpenoids as a consequence of gut bacterial metabolisms, could be used as recruitment signals (produced by both bacterial communities) or sex pheromones (produced by the bacterial community from female individuals) by insects.…”

Section: Discussionmentioning

confidence: 99%

Cultivable gut bacteria provide a pathway for adaptation of Chrysolina herbacea to Mentha aquatica volatiles

et al. 2017

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BackgroundA chemical cross-talk between plants and insects is required in order to achieve a successful co-adaptation. In response to herbivory, plants produce specific compounds, and feeding insects respond adequately7 to molecules produced by plants. Here we show the role of the gut microbial community of the mint beetle Chrysolina herbacea in the chemical cross-talk with Mentha aquatica (or watermint).ResultsBy using two-dimensional gas chromatography–mass spectrometry we first evaluated the chemical patterns of both M. aquatica leaf and frass volatiles extracted by C. herbacea males and females feeding on plants, and observed marked differences between males and females volatiles. The sex-specific chemical pattern of the frass paralleled with sex-specific distribution of cultivable gut bacteria. Indeed, all isolated gut bacteria from females belonged to either α- or γ-Proteobacteria, whilst those from males were γ-Proteobacteria or Firmicutes. We then demonstrated that five Serratia marcescens strains from females possessed antibacterial activity against bacteria from males belonging to Firmicutes suggesting competition by production of antimicrobial compounds. By in vitro experiments, we lastly showed that the microbial communities from the two sexes were associated to specific metabolic patterns with respect to their ability to biotransform M. aquatica terpenoids, and metabolize them into an array of compounds with possible pheromone activity.ConclusionsOur data suggest that cultivable gut bacteria of Chrysolina herbacea males and females influence the volatile blend of herbivory induced Mentha aquatica volatiles in a sex-specific way.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-0986-6) contains supplementary material, which is available to authorized users.

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“…Intriguingly, there is evidence that root-associated bacteria may be involved in the biosynthesis of several constituents of the vetiver oil. These bacteria, living in the essential oil-producing cells and in the lysigen lacunae in close association with the essential oil, metabolize biosynthetic precursors that are synthetized by the plant [85,86]. This is supported by evidence that axenic vetiver produced only trace amounts of oil in vitro, with a strikingly different composition compared to the oils from in vivo vetiver plants.…”

Section: Bacteria and Insect-plant Communicationmentioning

confidence: 96%

“…This is supported by evidence that axenic vetiver produced only trace amounts of oil in vitro, with a strikingly different composition compared to the oils from in vivo vetiver plants. Most root-isolated bacteria were grown using oil sesquiterpenes as a carbon source and were biotrasformed into a large number of compounds typically found in commercial vetiver oils [85] ( Table S1). Some of these compounds have repellent activity to insects, such as bicyclovetivenol, which was synthesized in vitro by vetiver root-associated Pseudomonas sp.…”

Section: Bacteria and Insect-plant Communicationmentioning

confidence: 99%

Bacterial Semiochemicals and Transkingdom Interactions with Insects and Plants

Calcagnile

Tredici

Talà

et al. 2019

Insects

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A peculiar feature of all living beings is their capability to communicate. With the discovery of the quorum sensing phenomenon in bioluminescent bacteria in the late 1960s, it became clear that intraspecies and interspecies communications and social behaviors also occur in simple microorganisms such as bacteria. However, at that time, it was difficult to imagine how such small organisms—invisible to the naked eye—could influence the behavior and wellbeing of the larger, more complex and visible organisms they colonize. Now that we know this information, the challenge is to identify the myriad of bacterial chemical signals and communication networks that regulate the life of what can be defined, in a whole, as a meta-organism. In this review, we described the transkingdom crosstalk between bacteria, insects, and plants from an ecological perspective, providing some paradigmatic examples. Second, we reviewed what is known about the genetic and biochemical bases of the bacterial chemical communication with other organisms and how explore the semiochemical potential of a bacterium can be explored. Finally, we illustrated how bacterial semiochemicals managing the transkingdom communication may be exploited from a biotechnological point of view.

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Microbes at work in perfumery: the microbial community of vetiver root and its involvement in essential oil biogenesis

Cited by 15 publications

References 10 publications

Chemometric analysis reveals links in the formation of fragrant bio-molecules during agarwood (Aquilaria malaccensis) and fungal interactions

Chemometric analysis reveals links in the formation of fragrant bio-molecules during agarwood (Aquilaria malaccensis) and fungal interactions

Cultivable gut bacteria provide a pathway for adaptation of Chrysolina herbacea to Mentha aquatica volatiles

Bacterial Semiochemicals and Transkingdom Interactions with Insects and Plants

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