Microbes and their products as novel therapeutics in medical applications

Chitra, J.; Rajendren, S.; Jeyakanthan, J.; Balakrishnan, Gopalsamy; Mercy, J. Jeba; Manikandan, N.; Kumari, Nkp

doi:10.1016/b978-0-323-90958-7.00019-4

Cited by 3 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzyme synthesis was triggered by KH2PO4 (1.2g), MgSO4 (0.6g), FeSO4 (0.005g), and KCL (0.4g). As compared to bacterial L-glutaminase made from Escherichia coli or Bacillus cereus, this fungal enzyme demonstrated greater efficiency as an oncolytic agent against acute lymphocytic leukaemia and hepatic cancer (Chitra et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Screening and Exhibition of Fungal L-glutaminase Enzyme as an Anticancer Agent in Different Egypt Soil Environment

Kassab

2023

Middle East Journal of Medical Genetics

View full text Add to dashboard Cite

Background: Acute lymphoblastic leukaemia and hepatic auxotrophic carcinoma for L-glutaminase are two of the leading causes of mortality worldwide. The bacterial L-glutaminase enzyme, which is normally produced under harsh circumstances from Escherichia coli or Bacillus cereus, was used to treat acute lymphoblastic leukaemia. Fungal L-glutaminase is preferred over bacterial L-glutaminase due to less hypersensitivity events, such as allergic responses and medicine neutralization; as well as higher efficiency against hepatic carcinoma. Aim of Study:The objective of the present work was to produce fungal L-glutaminase enzyme from different soil types in Egypt to be used as an oncolytic agent. Patients and Methods: In the current screening experiment, mineral L-glutamine agar (MGA), a selective medium, was employed for the production of fungal L-glutaminase. Malt agar media was further used to sub-cultivate fungal L-glutaminase producing strains. L-glutaminase-producing strains were molecularly identified by using the Southern blotting method. The fungal L-glutaminase gene was firstly isolated using specific PCR primers then the gene was sub-cloned and inserted into a DNA vector to be produced via recombinant DNA technology. In order to assess anticancer activity, the MTT test was performed. Molecular mass of L-glutaminase was assessed via a mass spectrometer; then confirmed with western blot assay. Results: Aspergillus niger Strain ATCC 1015 was identified as the major fungal isolate that produced this enzyme. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay test results showed that the IC50 values for anticancer activity against the cancer cell lines CCL-120 and JHH4 were 38.9 and 40.3 g/ml, respectively. L-glutaminase had a molecular mass of 65 kDa, a specific activity of 15.3 U/mg protein, a yield of 57.6%, and a purity factor of 3.8. Extracellular L-glutaminase, 6.8U/ml, was generated. The conventional vitamin C and L-glutaminase have IC50s for their antioxidant activity of 89 g/ml and 189 g/ml, respectively. The inoculum contained 1*10 8 spores/ml. Conclusion: As fungus in diverse Egyptian soil environments evolved into a novel source of the enzyme L-glutaminase, the current investigation was a promising one.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening and Exhibition of Fungal L-glutaminase Enzyme as an Anticancer Agent in Different Egypt Soil Environment

Kassab

2023

Middle East Journal of Medical Genetics

View full text Add to dashboard Cite

show abstract

“…Therefore, the application of soil bacteria's open up new areas of biotechnological exploitations, which lead to the essentials of isolation and cultivation of these organisms [63,64]. They produce a variety of vaccines, antibiotics, steroids, and therapeutically useful compounds with strong biological activities [65]. Streptomycin was the first aminoglycoside antibiotic derived from the soil bacterium Streptomyces griseus.…”

Section: Antimicrobial Metabolites From Soil Bacteriamentioning

confidence: 99%

Antimicrobial and anticancer potential of soil bacterial metabolites - a comprehensive and updated review

Kumar¹,

Kumaresan²

2023

J App Biol Biotech

View full text Add to dashboard Cite

The majority of natural products currently used in the medical field are derived from microbial or plant sources. The bioactive compounds derived from natural sources exhibit tremendous structural and chemical diversity. According to previous research, only a small percentage of the world's plant and microbial diversity has been examined for bioactivities. The compounds originating from secondary metabolites of microorganisms are more useful for the development of novel drugs due to their biological friendliness and drug-likeness than any other compounds. Thus, recent research suggests that microorganisms obtained from diverse habitats and natural resources offer various bioactive secondary metabolites with incredibly wider chemical entities, hopefully, an alternative remedy for many diseases. Soil bacteria are capable of producing a variety of natural bioactive compounds for the treatment of various diseases. The three genera Bacillus spp., Streptomyces spp., and Pseudomonas spp. have been the prime focus to produce different types of antibiotics. However, to date, there are no reviews that evaluated the antimicrobial and anticancer properties of soil bacterial metabolites. Hence, the current review aimed to assess the antimicrobial and anticancer potential of soil bacterial metabolites.

show abstract

“…Besides, the potential medical properties of these identified strains may pave the way for future therapeutic applications. [17][18][19] In the present study, kurut samples (fermented cow milk from Kyrgyzstan) (n = 84) were analyzed via a metagenomics approach, and the bacterial composition of the samples was completely identified. To the best of our knowledge, this is one of the most comprehensive study conducted in kurut samples.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it is indispensable to characterize kurut's microbial diversity and identify new strains for the design and development of starter cultures in industrial applications. Besides, the potential medical properties of these identified strains may pave the way for future therapeutic applications [17–19] …”

Section: Introductionmentioning

confidence: 99%

A metagenomic survey of bacterial communities from kurut: The fermented cow milk in Kyrgyzstan

Yegin,

Mamatova,

Yurt

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

Kurut is a traditional dry dairy product mostly consumed in Central Asia. In this study, the distribution of the dominant bacteria present in kurut samples (n=84) originated from seven (Chuy, Issyk‐Kul, Talas, Naryn, Jalal‐Abad, Osh, and Batken) regions in Kyrgyzstan were analyzed with Illumina iSeq100 platform. The dominant phylum detected was Firmicutes followed by Proteobacteria, Actinobacteria, Cyanobacteria/Chloroplast, and Tenericutes. The most abundant family detected was Lactobacillaceae followed by Streptococcaceae, Enterococcaceae, Chloroplast, and Leuconostocaceae. At the genus level, Lactobacillus was the predominant one in samples and Streptococcus, Enterococcus, Lactococcus, and Streptophyta followed this. Further comprehensive characterization analyses in kurut samples may have potential applications both in industrial starter culture developments and also future therapeutic approaches based on potential strains with probiotic properties.

show abstract

Microbes and their products as novel therapeutics in medical applications

Cited by 3 publications

References 28 publications

Screening and Exhibition of Fungal L-glutaminase Enzyme as an Anticancer Agent in Different Egypt Soil Environment

Screening and Exhibition of Fungal L-glutaminase Enzyme as an Anticancer Agent in Different Egypt Soil Environment

Antimicrobial and anticancer potential of soil bacterial metabolites - a comprehensive and updated review

A metagenomic survey of bacterial communities from kurut: The fermented cow milk in Kyrgyzstan

Contact Info

Product

Resources

About