Microbeam-irradiated tumour tissue possesses a different infrared absorbance profile compared to broad beam and sham-irradiated tissue

Sharma, Monica; Crosbie, Jack; Puškar, Ljiljana; Rogers, Peter A. W.

doi:10.3109/09553002.2012.721052

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…36,44 This stress could be enhanced by the increased generation of ROS due to the presence of AuNP, which has been associated with changes in the amide I and II bands. [44][45][46] An amplification in the apoptotic response of irradiated cells in the presence of AuNP is in accordance with our previous FTIRM study using gadolinium NP. 21,27 The protein modifications as a function of the treatment are clearly shown on the vector normalized Savitzky-Golay second derivative plot ( Fig.…”

Section: Sr-ftirm Study On the Radiosensitization Effects At Several supporting

confidence: 91%

A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

Martínez-Rovira

Seksek

Yousef

2019

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Section: Sr-ftirm Study On the Radiosensitization Effects At Several supporting

confidence: 91%

A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

Martínez-Rovira

Seksek

Yousef

2019

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“…The tissue composition of the EMT6.5 mammary model was investigated after MRT (25 µm; 200 ctc; 560 Gy peak-dose) and BB (11, 22 or 44 Gy) using Fourier-transform infrared microspectroscopy [54]. Absorbance patterns in the nucleic acid region showed chemical shifts between the peak and valley-dose regions [54].…”

Section: Mammary Tumoursmentioning

confidence: 99%

Animal Models in Microbeam Radiation Therapy: A Scoping Review

Fernández-Palomo

Fazzari

Trappetti

et al. 2020

Cancers

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Background: Microbeam Radiation Therapy (MRT) is an innovative approach in radiation oncology where a collimator subdivides the homogeneous radiation field into an array of co-planar, high-dose beams which are tens of micrometres wide and separated by a few hundred micrometres. Objective: This scoping review was conducted to map the available evidence and provide a comprehensive overview of the similarities, differences, and outcomes of all experiments that have employed animal models in MRT. Methods: We considered articles that employed animal models for the purpose of studying the effects of MRT. We searched in seven databases for published and unpublished literature. Two independent reviewers screened citations for inclusion. Data extraction was done by three reviewers. Results: After screening 5688 citations and 159 full-text papers, 95 articles were included, of which 72 were experimental articles. Here we present the animal models and pre-clinical radiation parameters employed in the existing MRT literature according to their use in cancer treatment, non-neoplastic diseases, or normal tissue studies. Conclusions: The study of MRT is concentrated in brain-related diseases performed mostly in rat models. An appropriate comparison between MRT and conventional radiotherapy (instead of synchrotron broad beam) is needed. Recommendations are provided for future studies involving MRT.

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“…It is possible that the MRT peak and valley dose induce different tissue responses compared to uniform CRT (7,8). Our group has recently demonstrated structural (conformational) differences after irradiation with MRT versus CRT using infrared spectroscopy (39). Changes were observed in three radiobiologically significant regions: proteins (1654-1630 cm À1 ), lipids (1474 and 1462 cm À1 ) and nucleic acids (1244-1050 cm À1 ), characteristic of functional group vibrations (-C ¼ O, -C-N, -N-H, -C-O, -PO 2-, CH 2 ) in protein secondary structures (e.g., Amide I), DNA and RNA molecules and lipids (39).…”

Section: Discussionmentioning

confidence: 99%

“…Our group has recently demonstrated structural (conformational) differences after irradiation with MRT versus CRT using infrared spectroscopy (39). Changes were observed in three radiobiologically significant regions: proteins (1654-1630 cm À1 ), lipids (1474 and 1462 cm À1 ) and nucleic acids (1244-1050 cm À1 ), characteristic of functional group vibrations (-C ¼ O, -C-N, -N-H, -C-O, -PO 2-, CH 2 ) in protein secondary structures (e.g., Amide I), DNA and RNA molecules and lipids (39). The finding that MRT causes conformational changes in secondary structure of proteins that are different from CRT raises the possibility that biological functions such as gene transcription, protein (enzyme) function, signaling and cell-cell interaction could be differentially affected.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Genes and Molecular Pathways Differentially Regulated by Synchrotron Microbeam Radiotherapy

et al. 2014

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The aim of this study was to identify genes and molecular pathways differentially regulated by synchrotron-generated microbeam radiotherapy (MRT) versus conventional broadbeam radiotherapy (CRT) in vitro using cultured EMT6.5 cells. We hypothesized (based on previous findings) that gene expression and molecular pathway changes after MRT are different from those seen after CRT. We found that at 24 h postirradiation, MRT exerts a broader regulatory effect on multiple pathways than CRT. MRT regulated those pathways involved in gene transcription, translation initiation, macromolecule metabolism, oxidoreductase activity and signaling transduction in a different manner compared to CRT. We also found that MRT/CRT alone, or when combined with inflammatory factor lipopolysaccharide, upregulated expression of Ccl2, Ccl5 or Csf2, which are involved in host immune cell recruitment. Our findings demonstrated differences in the molecular pathway for MRT versus CRT in the cultured tumor cells, and were consistent with the idea that radiation plays a role in recruiting tumor-associated immune cells to the tumor. Our results also suggest that a combination of MRT/CRT with a treatment targeting CCL2 or CSF2 could repress the tumor-associated immune cell recruitment, delay tumor growth and/or metastasis and yield better tumor control than radiation alone.

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Microbeam-irradiated tumour tissue possesses a different infrared absorbance profile compared to broad beam and sham-irradiated tissue

Abstract: The lack of difference between MRT peak and valley irradiated areas suggests a holistic tissue response to MRT that occurs within 4 h, and might be the first evidence for a mechanism by which MRT kills the whole tumour despite only a small percentage receiving peak irradiation.

Cited by 10 publications

References 32 publications

A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

Animal Models in Microbeam Radiation Therapy: A Scoping Review

In Vitro Study of Genes and Molecular Pathways Differentially Regulated by Synchrotron Microbeam Radiotherapy

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