Microbeads on microposts: An inverted architecture for bead microarrays

Filipponi, Luisa; Sawant, Prashant D.; Fulga, Florin; Nicolau, Dan V.

doi:10.1016/j.bios.2008.09.015

Cited by 14 publications

(15 citation statements)

References 45 publications

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“…By applying different printing pressure, the width of the contact area increases and the height of the pyramid decreases (Hong et al 2008 ). The design of Si masters presenting pyramidal wells has been used before (Filipponi et al 2009 ; Hong et al 2008 ; Schwinger et al 2008 ), largely as a method to achieve very high resolution of the patterns without the high cost of ownership, or the need to fabricate very fine features in the Si master for PDMS replication.…”

Section: Resultsmentioning

confidence: 99%

“…As described elsewhere (Delamarche et al 1997 ), in order to avoid or mitigate the unwanted structural deformations during μCP, the aspect ratio of PDMS features has to be in the range of 0.2 to 2, and the inter-feature distance should not exceed 20 times their height. While the pyramidal profile of the PDMS stamps have been used occasionally in the past (Filipponi et al 2009 ; Hong et al 2008 ; Schwinger et al 2008 ), most of the above mentioned analyses refer to rectangular, or cylindrical profiles of the stamps.…”

Section: Resultsmentioning

confidence: 99%

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Protein patterning by microcontact printing using pyramidal PDMS stamps

Filipponi

Livingston

Kašpar

et al. 2016

Biomed Microdevices

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Micro-contact printing, μCP, is a well-established soft-lithography technique for printing biomolecules. μCP uses stamps made of Poly(dimethylsiloxane), PDMS, made by replicating a microstructured silicon master fabricated by semiconductor manufacturing processes. One of the problems of the μCP is the difficult control of the printing process, which, because of the high compressibility of PDMS, is very sensitive to minute changes in the applied pressure. This over-sensitive response leads to frequent and/or uncontrollable collapse of the stamps with high aspect ratios, thus decreasing the printing accuracy and reproducibility. Here we present a straightforward methodology of designing and fabricating PDMS structures with an architecture which uses the collapse of the stamp to reduce, rather than enlarge the variability of the printing. The PDMS stamp, organized as an array of pyramidal micro-posts, whose ceiling collapses when pressed on a flat surface, replicates the structure of the silicon master fabricated by anisotropic wet etching. Upon application of pressure, depending on the size of, and the pitch between, the PDMS pyramids, an air gap is formed surrounding either the entire array, or individual posts. The printing technology, which also exhibits a remarkably low background noise for fluorescence detection, may find applications when the clear demarcation of the shapes of protein patterns and the distance between them are critical, such as microarrays and studies of cell patterning.Electronic supplementary materialThe online version of this article (doi:10.1007/s10544-016-0036-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Protein patterning by microcontact printing using pyramidal PDMS stamps

Filipponi

Livingston

Kašpar

et al. 2016

Biomed Microdevices

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“…Another approach to achieve a better control of the printing process is to use pyramidal PDMS stamps in MicroCP [22][23][24][25]. The use of the pyramidal architecture for the PDMS stamps makes the collapse by buckling and by lateral sticking, i.e., two of the main collapse modes of PDMS stamps, irrelevant [26], and the pyramidal shape of the PDMS tip ensures a stable mechanical deformation for a controllable wide-range pattern generation.…”

Section: Introductionmentioning

confidence: 99%

Deformation of Pyramidal PDMS Stamps During Microcontact Printing

Jin

Qiao

2016

Journal of Applied Mechanics

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Microcontact printing (MicroCP) is a form of soft lithography that uses the relief patterns on a master polydimethylsiloxane (PDMS) stamp to form patterns of self-assembled monolayers (SAMs) of ink on the surface of a substrate through conformal contact. Pyramidal PDMS stamps have received a lot of attention in the research community in recent years, due to the fact that the use of the pyramidal architecture has multiple advantages over traditional rectangular and cylindrical PDMS stamps. To better understand the dynamic MicroCP process involving pyramidal PDMS stamps, in this paper, numerical studies on frictionless adhesive contact between pyramidal PDMS stamps and transversely isotropic materials are presented. We use a numerical simulation method in which the adhesive interactions are represented by an interaction potential and the surface deformations are coupled by using half-space Green's functions discretized on the surface. It shows that for pyramidal PDMS stamps, the contact area increases significantly with increasing applied load, and thus, this technique is expected to provide a simple, efficient, and low-cost method to create variable two-dimensional arrays of dot chemical patterns for nanotechnology and biotechnology applications. The DMT-type and Johnson–Kendall–Roberts (JKR)-type-to-DMT-type transition regimes have been explored by conducting the simulations using smaller values of Tabor parameters.

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“…Also, the long range self-assembly of proteins, e.g., cytoskeleton formation [12], formation of amyloid plaques and tangles [13], occurs through biomolecular recognition of larger areas on the molecular surface. Biomolecules also interact with solid surfaces on which they are immobilized, either by design, or unintentionally [14], [15], for applications as diverse as biomaterials [14], [16], chromatography [17] membrane research [18], [19], biomedical micro- and nano-devices [20], [21], such as biosensors [22], microarrays [23], [24] and lab-on-a-chip devices [25], where the preservation of the bioactivity of the immobilized proteins is paramount. More recently, nanoparticle research has become interested in the study of the interaction between proteins and artificial objects similar with their size, or larger [26].…”

Section: Introductionmentioning

confidence: 99%

Protein Molecular Surface Mapped at Different Geometrical Resolutions

2013

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Many areas of biochemistry and molecular biology, both fundamental and applications-orientated, require an accurate construction, representation and understanding of the protein molecular surface and its interaction with other, usually small, molecules. There are however many situations when the protein molecular surface gets in physical contact with larger objects, either biological, such as membranes, or artificial, such as nanoparticles. The contribution presents a methodology for describing and quantifying the molecular properties of proteins, by geometrical and physico-chemical mapping of the molecular surfaces, with several analytical relationships being proposed for molecular surface properties. The relevance of the molecular surface-derived properties has been demonstrated through the calculation of the statistical strength of the prediction of protein adsorption. It is expected that the extension of this methodology to other phenomena involving proteins near solid surfaces, in particular the protein interaction with nanoparticles, will result in important benefits in the understanding and design of protein-specific solid surfaces.

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Microbeads on microposts: An inverted architecture for bead microarrays

Cited by 14 publications

References 45 publications

Protein patterning by microcontact printing using pyramidal PDMS stamps

Protein patterning by microcontact printing using pyramidal PDMS stamps

Deformation of Pyramidal PDMS Stamps During Microcontact Printing

Protein Molecular Surface Mapped at Different Geometrical Resolutions

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