Microbe-mediated synthesis of antimicrobial semiconductor nanoparticles by marine bacteria

Rajeshkumar, S.; Ponnanikajamideen, M.; Malarkodi, Chelladurai; Malini, Madasamy; Annadurai, G.

doi:10.1007/s40097-014-0096-z

Cited by 72 publications

(39 citation statements)

References 18 publications

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“…Antimicrobial efficacy of AgNPs is generally evaluated by diffusion, dilution and bioautographic methods designed for rapid screening of primary assay . During the last years, flow cytometry has become a powerful technique employed mostly for discriminating living and dead eukaryotic cells .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

et al. 2016

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This work reports the effect of silver bionanoparticles (Bio(AgNPs) synthesized by Actinobacteria CGG 11n on selected Gram (+) and Gram (-) bacteria. Flow cytometry, classical antibiogram method and fluorescent microscopy approach was used for evaluation of antimicrobial activity of Bio(AgNPs) and their combination with antibiotics. Furthermore, the performed research specified the capacity of flow cytometry method as an alternative to the standard ones and as a complementary method to electromigration techniques. The study showed antibacterial activity of both BioAgNPs and the combination of antibiotics/BioAgNPs against all the tested bacteria strains in comparison with a diffusion, dilution and bioautographic methods. The synergistic effect of antibiotics/BioAgNPs combination (e.g. kanamycin, ampicillin, neomycin and streptomycin) was found to be more notable against Pseudomonas aeruginosa representing a prototype of multi-drug resistant "superbugs" for which effective therapeutic options are very limited.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

et al. 2016

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“…In addition, the availability of several biological macromolecules/substances that can serve as capping and stabilization molecules for the green synthesis of nanoparticles has also contributed to the steady rise of this process. Several authors have used bacteria, fungi, algae and plant extracts for the green synthesis of metal nanoparticles (Shivaji et al 2011;Salem et al 2014;Augustine et al 2014;Shanmugam et al 2014;Nazeruddin et al 2014;Rajeshkumar et al 2014;Raliya and Tarafdar 2014;Mishra et al 2014;Lateef et al 2015a, b;El-Batal et al 2015). However, there is lack of information on the use of metabolites of arthropods for the green synthesis of nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

et al. 2015

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In this study, spider cobweb as a novel biomaterial was used for the green synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The efficacy of biosynthesized particles as antibacterial agents was evaluated using multidrug resistant clinical bacterial isolates through sensitivity testing with AgNPs and combination of AgNPs with some selected antibiotics. In addition, the potential application of the particles as additives in paints was demonstrated using some bacterial and fungal isolates. The synthesized AgNPs which were dark brown in color displayed maximum absorbance at the wavelength of 436 nm. It was observed that the reaction mixture of 1:40 (extract:AgNO 3 solution) at pH of 8.5 produced particles with maximum absorbance at 436 nm. The FTIR spectrum showed peaks at 3298, 2359, 2089, and 1635 cm -1 , indicating that proteins were the capping and stabilization molecules in the synthesis of AgNPs. The particles were spherical in shape with size ranging about 3-50 nm. The energy-dispersive X-ray analysis showed the presence of silver as the most prominent metal, while the selected area electron diffraction pattern conformed to the face-centered cubic phase and crystalline nature of AgNPs. The AgNPs inhibited the growth of several bacterial isolates including S. aureus, E. coli, Klebsiella granulomatis and P. aeruginosa in the range of 10-17 mm at concentration of 100 lg/ml. It was also demonstrated that AgNPs potentiated the activities of augmentin, ofloxacin and cefixime in the AgNP-antibiotic synergy studies. Similarly, the inclusion of AgNPs as additive in white emulsion paint led to the total inhibition of growth of E. coli, P. aeruginosa, Aspergillus niger and A. fumigatus. To the best of our knowledge, this is the first report of the use of cobweb for the green synthesis of AgNPs. The immense antimicrobial activities of the particles can be explored in the creation of novel products, where it can be used as additive to protect materials against microbial attack.

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“…Metal nanoparticles and their alloys such as silver, iron, cadmium, zinc, platinum, gold and graphene have diverse applications in different aspects of human endeavours, such as catalytic activity [9,22,23], optical [24], electronic [25], magnetic, antimicrobial [3,7,10,13,26] and biomedical usefulness [27,28]. AgNPs, for instance, are of interest because of their unique properties (size-and shape-dependent optical, electrical and magnetic properties) which can be incorporated into coating materials, antimicrobial applications, biosensor materials, composite fibres and electronic components [29].…”

Section: Introductionmentioning

confidence: 99%

Cola nitida-Mediated Biogenic Synthesis of Silver Nanoparticles Using Seed and Seed Shell Extracts and Evaluation of Antibacterial Activities

et al. 2015

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In this study, seed and seed shell extracts of Cola nitida were investigated for the biogenic synthesis of silver nanoparticles (AgNPs) under ambient condition. The biosynthesized AgNPs were characterized through visual development of colour, UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The antibacterial activities of the AgNPs were determined using some multidrug-resistant clinical isolates. The biosynthesized AgNPs depicted brown and yellowish orange colour using seed and seed extract, respectively, with maximum absorbance readings at 457.5 and 454.5 nm. The FTIR spectrum showed strong peaks at 3292.49, 2086.98 and 1631.78 cm −1 for seed extract-mediated AgNPs, while peaks of 3302.13, 2086.05 and 1633.71 cm −1 were obtained for seed shell extract-mediated AgNPs, all indicating that proteins were the capping and stabilization molecules in the biogenic synthesis of AgNPs. The AgNPs were spherical in shape with sizes ranging from 8 to 50 and 5 to 40 nm for seed and seed shell-mediated AgNPs, respectively. The energydispersive X-ray (EDX) analysis showed the presence of silver as a prominent metal, while the selected area electron diffraction (SAED) pattern conformed to the face-centred cubic phase and crystalline nature of AgNPs. At various concentrations ranging from 50-150 μg/ml, the AgNPs inhibited growth of multidrug-resistant strains of Klebsiella granulomatis, Pseudomonas aeruginosa and Escherichia coli to the tune of 10-32 mm. Comparatively, seed shell extractmediated AgNPs had better activities with minimum inhibitory concentration (MIC) of 50 μg/ml against all the tested isolates, while the MICs of seed extract-mediated AgNPs were obtained as 50, 80 and 120 μg/ml against E. coli, P. aeruginosa (wound) and P. aeruginosa (burn), respectively. This study has demonstrated the feasibility of eco-friendly biogenic synthesis of AgNPs using seed and seed shell extracts of C. nitida, and the report to the best of our knowledge is the first reference to extracts of C. nitida for the green synthesis of AgNPs.

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Microbe-mediated synthesis of antimicrobial semiconductor nanoparticles by marine bacteria

Cited by 72 publications

References 18 publications

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

Cola nitida-Mediated Biogenic Synthesis of Silver Nanoparticles Using Seed and Seed Shell Extracts and Evaluation of Antibacterial Activities

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