Microbe hunting in the joints

Zhang, Xiang; Pacheco‐Tena, César; Inman, Robert D.

doi:10.1002/art.11186

Cited by 19 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial LPS has been detected in neutrophils and macrophages for prolonged periods after the inciting infection, although synovial fibroblasts were not specifically examined (5). We have recently reviewed the several lines of evidence for the persistence of microbes or their products within the joints in ReA (21). Defining the initial phases of ReA has proved to be difficult in the clinical setting, and we used two approaches to analyze these hostpathogen interactions.…”

mentioning

confidence: 99%

Synovial Fibroblasts Infected with Salmonella enterica Serovar Typhimurium Mediate Osteoclast Differentiation and Activation

et al. 2004

Self Cite

View full text Add to dashboard Cite

The mechanisms whereby arthritogenic organisms may induce cartilage and bone erosions in infectiontriggered arthritis remain unknown. In this study, we asked whether an arthritogenic organism could contribute to osteoclast differentiation and activation through regulation of the receptor activator of NF-B ligand (RANKL) in synovial fibroblasts. Rat synovial fibroblasts were infected in vitro with Salmonella enterica serovar Typhimurium and monitored over time. The expression of RANKL in resting and infected synovial fibroblasts was quantified by reverse transcription-PCR and Western blotting. Osteoclast progenitors, isolated from femurs of 8-week-old rats and cultured in the presence of macrophage colony-stimulating factor, were cocultured with either infected or noninfected synovial fibroblasts for 2 to 4 days. Differentiation and maturation of osteoclasts were determined by morphology and tartrate-resistant acid phosphatase (TRAP) staining and by a bone resorption bioassay. RANKL expression was undetectable in resting synovial fibroblasts but was dose-dependently upregulated in cells after Salmonella infection. Osteoprotegerin was constitutively expressed by synovial fibroblasts and was not upregulated by infection. Further, we observed the formation of multinucleated TRAP-positive cells and formation of bone resorption pits in cocultures of bone marrow-derived osteoclast precursors with synovial fibroblasts infected with Salmonella but not with heat-killed Salmonella or noninfected cells. Arthritogenic bacteria may alter bone structure via synovial fibroblast intermediaries, since infected synovial fibroblasts (i) upregulate RANKL expression and (ii) enhance osteoclast precursor maturation into multinucleated, TRAP-positive, bone-resorbing, osteoclast-like cells. These data provide a link between infection and osteoclastogenesis. A better understanding of infection-mediated osteoclast differentiation and activation may provide new therapeutic strategies for inflammatory joint disease.

show abstract

mentioning

confidence: 99%

Synovial Fibroblasts Infected with Salmonella enterica Serovar Typhimurium Mediate Osteoclast Differentiation and Activation

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…New analytical techniques have been applied to probing synovial fluids and tissue for evidence of prior or current microbes [52,53]. Invasion by Salmonella, however, did not alter the B27 peptide presentation profile [54].…”

Section: Bacteriamentioning

confidence: 98%

Pathogenesis of ankylosing spondylitis and reactive arthritis

Kim

Uhm

Inman

2005

Current Opinion in Rheumatology

View full text Add to dashboard Cite

show abstract

“…There is also evidence that Chlamydia spp can alter host response to the organisms by inhibition of host cell apoptosis, by reducing the release of cytochrome C, and by sequestering protein kinase C delta in the membrane of the organisms' vacuoles (16). Newer analytic techniques are being used to probe synovial fl uids and tissues for evidence of prior or current microbes (17,18).…”

Section: Pathogenesis Of Reactive Arthritismentioning

confidence: 99%