Microbatch crystallization under oil — a new technique allowing many small-volume crystallization trials

Chayen, Naomi E.; Stewart, Patrick D. Shaw; Blow, D. M.

doi:10.1016/0022-0248(92)90241-a

Cited by 232 publications

(186 citation statements)

References 8 publications

Supporting

Mentioning

177

Contrasting

Unclassified

Order By: Relevance

“…Crystallization trials started with hanging drop vapor diffusion (Ducruix & Gieg&, 1992) and matrix screens (Jancarik & Kim, 1991). Crude optimization was by: (1) vapor diffusion/incomplete factorial trials (Carter, 1992) for combinations of PEG/isopropanol/Mg'+; (2) binary search (Stewart & Khimasia, 1994) and grids using the microbatch method (Chayen et al, 1992) and 1-2 p L drops of concentrate protein (>20 mg/mL) with which buffer (various: pH 6.5-10) and crystallizing agent were mixed.…”

Section: Methodsmentioning

confidence: 99%

“…The largest (0.3 X 0.1 X 0.05 mm3). obtained by microbatch crystallization (Chayen et al, 1992) with 12% PEG 6000 or 23% PEG 1000. diffracted X-rays to -3; 8,.…”

Section: Purification Of a K From A Natural Source (Horseshoe Crabs) mentioning

confidence: 99%

“…The following were compared in controlled trials: (1) the microbatch (Chayen et al, 1992) and vapor diffusion methods (Ducruix & GiegC, 1992); (2) temperatures of 4" and 23°C; (3) PEG molecular weights of 3,000 to 12,000 D; (4) Monomethyl ether substituted PEGS (Brzozowski & Tolley, 1994;Patel et al, 1995) and unsubstituted PEGS; (5) salt additives at 50, 100, and 200 mMNaCl, MgCl2, Na3C6H507, Li2SO4, Mg(C2H302h (NHd2S04, NH4Cl, (NH4)3C6Hs07r all adjusted to pH 7.5 with isomolar solutions of the conjugate acid/base; (6) sample filtration-none compared to use of 0.22 p m and 300 kDa membrane filters (Blow et al, 1994); (7) detergent additives (McPherson et al, 1986): up to 0.1 % w/v P-octyl glucoside, nonyl-P-glucopyranoside or decyl maltoside; (8) organic co-solvents: 5% ethanol, isopropanol, or hydroxyacetone.…”

Section: Optimization Of Crystallization For Recombinant Akmentioning

confidence: 99%

See 2 more Smart Citations

Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase: A model for studying phosphagen kinases

et al. 1997

View full text Add to dashboard Cite

Phosphagen kinases catalyze the reversible transfer of a phosphoryl group between guanidino phosphate compounds and ADP, thereby regenerating ATP during bursts of cellular activity. Large quantities of highly pure arginine kinase (EC 2.7.3.3), the phosphagen kinase present in arthropods, have been isolated from E. coli, into which the cDNA for the horseshoe crab enzyme had been cloned. Purification involves size exclusion and anion exchange chromatographies applied in the denatured and refolded states. The recombinant enzyme has been crystallized as a transition state analog complex. Near complete native diffraction data have been collected to 1.86 A resolution. Substitution of a recombinant source for a natural one, improvement in the purification, and data collection at cry0 temperatures have all yielded significant improvements in diffraction. This reaction and those of the rest of the guanidino (or phosphagen) kinase family, including creatine kinase (CK), enable ATP to be quickly regenerated from storage phosphagens during bursts of cellular activity. In addition to this "temporal" buffering, it has been proposed that these enzymes also function in "spatial" buff-

show abstract

Section: Methodsmentioning

confidence: 99%

“…The largest (0.3 X 0.1 X 0.05 mm3). obtained by microbatch crystallization (Chayen et al, 1992) with 12% PEG 6000 or 23% PEG 1000. diffracted X-rays to -3; 8,.…”

Section: Purification Of a K From A Natural Source (Horseshoe Crabs) mentioning

confidence: 99%

Section: Optimization Of Crystallization For Recombinant Akmentioning

confidence: 99%

See 1 more Smart Citation

Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase: A model for studying phosphagen kinases

et al. 1997

View full text Add to dashboard Cite

show abstract

“…All crystallization trials used the microbatch crystallization technique under oil described by Chayen et al (1992). Crystallization samples were dispensed and stored under paraffin oil (BDH paraffin liquid, colorless light GPR cat.…”

Section: Crystallizationmentioning

confidence: 99%

Control of nucleation in the crystallization of lysozyme

1993

Self Cite

View full text Add to dashboard Cite

This work investigates the influence of storage of lysozyme in solution on its crystallization. The crystallization of hen egg-white lysozyme exhibits a storage effect (aging) that depends on the length of time the lysozyme solution is stored, after dissolving from freeze-dried powder, before being brought to crystallization conditions. The number of crystals obtained increases, while their size decreases, as the solution ages. Observations suggest that this effect is due to the presence of fungi that multiply in the stored protein solution. This aging effect was used to control nucleation and determine the number and size of lysozyme crystals to be formed in a given sample.

show abstract

“…An initial crystallization condition was found using the Wizard II crystal screen kit (Jena Bioscience, Germany) using the microbatch method under oil [15] and an Oryx 6 crystallization robot (Douglas Instruments, UK). Protein solution (1 l, 8.28 mg/ml) was mixed with 1 l of reservoir solution containing 2 M phosphate (0.8 M NaH 2 PO 4 /1.2 M K 2 HPO 4 ) and 0.1 M Na-acetate, pH 6.7.…”

Section: Crystallization Of Atmentioning

confidence: 99%

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: Isopenicillin N acyltransferase from Penicillium chrysogenum

Yoshida

Hensgens²,

Laan

et al. 2005

Protein Expression and Purification

View full text Add to dashboard Cite

Microbatch crystallization under oil — a new technique allowing many small-volume crystallization trials

Cited by 232 publications

References 8 publications

Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase: A model for studying phosphagen kinases

Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase: A model for studying phosphagen kinases

Control of nucleation in the crystallization of lysozyme

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: Isopenicillin N acyltransferase from Penicillium chrysogenum

Contact Info

Product

Resources

About