Microbacterium sulfonylureivorans sp. nov., isolated from sulfonylurea herbicides degrading consortium

Abstract

A novel Gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7116 T , was isolated from a sulfonylurea herbicides degrading consortium enriched with the birch forest soil from Xinjiang. The optimal temperature and pH for the growth of strain LAM7116 T were 35 °C and 7.0, respectively. Strain LAM7116 T could grow in the presence of NaCl up to 4% (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain LAM7116 T was closely related to the members of the genus Microbacterium, with the highest similarity to Microbacterium avescens DSM 20643 T (98.1%) and Microbacterium kitamiense Kitami C2 T (98.1 %). Strain LAM7116 T formed a distinct subclade with M. avescens DSM 20643 T within the genus Microbacterium in the 16S rRNA gene phylogenetic trees. The genomic DNA G+C content of LAM7116 T was 69.9 mol%. The DNA-DNA hybridization (DDH) value between strain LAM7116 T and M. avescens DSM 20643 T was 27.20 %. The average nucleotide identity (ANI) value was 83.96 % by comparing the draft genome sequences of strain LAM7116 T and M. avescens DSM 20643 T . The major fatty acids were anteiso-C 15:0 , anteiso-C 17:0 , iso-C 17:0 and iso-C 16:0 . The respiratory quinones of strain LAM7116 T were MK-13 and MK-14. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidenti ed lipid and an unidenti ed glycolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7116 T represents a novel species, for which the name Microbacterium sulfonylureivorans sp. nov. was proposed. The type strain is LAM7116 T (=CGMCC 1.16620 T =JCM 32823 T ). Strain LAM7116 T secreted auxin IAA and grew well in Ashby nitrogen-free culture medium. Genomic results showed that strain LAM7116 T carried the nitrogenase iron protein (nifU and nifR3) gene, which indicated that strain LAM7116 T has the potential to x nitrogen and promote plant growth. At the same time, the strain LAM7116 T can degrade nicosulfuron (a kind of sulfonylurea herbicides) by using glucose as carbon source, microbial degradation of nicosulfuron is the primary process of removing nicosulfuron from environments, and biodegradation is the most effective and environmentally friendly. Microbacterium sulfonylureivorans sp. nov. LAM7116 T is a potential candidate for the biofertilizers of organic agriculture areas, having broad prospects for using, and may possess potential to be used in bioremediation of nicosulfuron-contaminated environments.

Effect of dietary supplementation Ampelopsis grossedentata extract on growth performance and muscle nutrition of Megalobrama hoffmanni by gut bacterial mediation

“…Consistently, Propionibacteriaceae (v55) was the keystone specie based on random forest test. Meanwhile, other keystone species also revealed relationships with fatty acids transformation, such as Acidimicrobiales related to PA (C16:0) [ 42 ], Demequina related to C15:0 and C16:0 [ 40 ], Rhodobacter related to C18:1ω7c/C18:1ω6c [ 43 ], Gemmataceae related to C18:1ɷ7c, C18:0, and β OH-C16:0 [ 44 ], and Microbacterium related to C15:0, C17:0, iso-C17:0, and iso-C16:0 [ 45 ]. Moreover, functional profiles imputed by PICRUSt2 were selected out and six functional profiles showed significant correlations with keystone species, which were involvement with fatty acid biosynthesis (ko00061), steroid biosynthesis (ko00100), tyrosine metabolism (ko00350), glycerolipid metabolism (ko00561), glycerophospholipid metabolism (ko00564), and linoleic acid metabolism (ko00591).…”

Genomic and metabolomic profiling reveal Streptomyces rochei S32 contributes to plant growth by nitrogen fixation and production of bioactive substances

“…Nitrogen xation. S. rochei S32 was inoculated onto Ashby's agar plates containing no nitrogen source (Ma et al 2022). Colony growth was observed and recorded after incubation at 30℃ for 96 h. If colonies formed on the plates, they were transferred to fresh Ashby's agar plates and continuously subcultured three times.…”