Microbacterium stercoris sp. nov., an indole acetic acid-producing actinobacterium isolated from cow dung

Zhang, Lida; Jiao, Yanjie; Ling, Ling; Wang, Han; Song, Wenshuai; Zhao, Tianxin; Guo, Liyun; Xiang, Wensheng; Zhao, Junwei; Wang, Xiangjing

doi:10.1099/ijsem.0.005099

“…After incubating at 28°C for 7 days, actinomycete-like colonies were picked for further purification and culture. Pure bacterial cultures were stored in 30% (v/v) -glycerol and kept at −80°C for further use ( Zhang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Characterization and evaluation of actinomycete from the Protaetia brevitarsis Larva Frass

Zhang,

Zhao,

Geng

et al. 2024

Front. Microbiol.

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Protaetia brevitarsis larvae (PBL) are soil insects important for the soil organic carbon cycle, and PBL frass not only contains a large amount of humic acid but also affects the diversity, novelty, and potential functions of actinomycetes. Here, we characterized and assessed the actinomycete. The operational taxonomic unit (OTU) data showed that 90% of the actinomycetes cannot be annotated to species, and pure culture and genome analysis showed that 35% of the strains had the potential to be new species, indicating the novelty of PBL frass actinomycetes. Additionally, genome annotation showed that many gene clusters related to antifungal, antibacterial and insecticidal compound synthesis were identified, and confrontation culture confirmed the antifungal activities of the actinomycetes against soil-borne plant pathogenic fungi. The incubation experiment results showed that all isolates were able to thrive on media composed of straw powder and alkaline lignin. These results indicated that PBL hindgut-enriched actinomycetes could survive in soil by using the residual lignocellulose organic matter from plant residues, and the antibiotics produced not only give them a competitive advantage among soil microflora but also have a certain inhibitory effect on plant diseases and pests. This study suggests that the application of PBL frass can not only supplement soil humic acid but also potentially affect the soil microbiota of cultivated land, which is beneficial for the healthy growth of crops.

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“…Some members of the genus Microbacterium are involved in a variety of functions such as the production of indole-3-acetic acid (IAA) [11], degradation of xylan [18–20], and solubilization of phosphate [18]. Additionally, they possess amylolytic [21], psychrotolerant, thermotolerant, halotolerant and alkalitolerant abilities [22], while providing UV radiation resistance [23], protecting against nematode pathogen [24] and promoting plant-growth [12].…”

Section: Introductionmentioning

confidence: 99%

“…Members of the genus Microbacterium are widely distributed in nature and have been isolated from various environmental sources, including air [9], activated sludge [9,10], animals [11], plants [12], fungi [13], water bodies [14,15], food [16] and so on [17]. Some members of the genus Microbacterium are involved in a variety of functions such as the production of indole-3-acetic acid (IAA) [11], degradation of xylan [18][19][20], and solubilization of phosphate [18].…”

Section: Introductionmentioning

confidence: 99%

“…Members of the genus Microbacterium are widely distributed in nature and have been isolated from various environmental sources, including air [9], activated sludge [9, 10], animals [11], plants [12], fungi [13], water bodies [14, 15], food [16] and so on [17].…”

Section: Introductionmentioning

confidence: 99%

“…Members of the genus Microbacterium are widely distributed in nature and have been isolated from various environmental sources, including air [9], activated sludge [9,10], animals [11], plants [12], fungi [13], water bodies [14,15], food [16] and so on [17]. Some members of the genus Microbacterium are involved in a variety of functions such as the production of indole-3-acetic acid (IAA) [11], degradation of xylan [18][19][20], and solubilization of phosphate [18]. Additionally, they possess amylolytic [21], psychrotolerant, thermotolerant, halotolerant and alkalitolerant abilities [22], while providing UV radiation resistance [23], protecting against nematode pathogen [24] and promoting plant-growth [12].…”

Section: Introductionmentioning

confidence: 99%

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Microbacterium nymphoidis sp. nov. and Microbacterium festucae sp. nov., two novel species with high plant-promoting potential isolated from wetland plants in China

Li,

Jin,

Yang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two novel plant growth-promoting, rod-shaped, Gram-positive and non-motile rhizobacteria, W1NT and W2RT, were isolated from wetland plants Festuca elata and Nymphoides peltatum, respectively, in China. The results of the 16S rRNA sequence alignment analysis showed that they were related to Microbacterium , with the highest similarity to Microbacterium ketosireducens (98.7 %) and Microbacterium laevaniformans (98.5 %) for strain W1NT, and to Microbacterium terricola (98.1 %) and Microbacterium marinum (98.0 %) for strain W2RT. Phylogenetic analyses based on 16S rRNA gene sequences and 92 conserved concatenated proteins suggested that the two strains belong to the genus Microbacterium and were placed in two separate novel phylogenetic clades. The genome sizes of the two strains were 3.2 and 3.7 Mb, and the G+C contents were 71.7 and 68.5 mol%, respectively. The comparative genome results showed that the average nucleotide identity values between W1NT and W2RT and other species ranged from 73.5 to 83.6 %, and the digital DNA–DNA hybridization values ranged from 19.7 to 26.8 %. These two strains show physiological and biochemical features that differ from those of closely related species. Rhamnose, galactose and glucose were present in the characteristic sugar fractions of strains W1NT and W2RT. The peptidoglycan of strains W1NT and W2RT contained the amino acids ornithine, alanine and aspartic acid. C15 : 0 anteiso, C17 : 0 anteiso and C16 : 0 iso were the predominant cellular fatty acids in W1NT and W2RT. Phosphatidylglycerol and diphosphatidylglycerol are major polar lipid components. Strain W1NT not only formed bacterial biofilms but also had the ability to solubilize phosphorus and produce indole-3-acetic acid. Strain W2RT had siderophore-producing and lignin-degrading properties. Based on their genetic and phenotypic characteristics, strains W1NT and W2RT were classified as novel bacteria in the genus Microbacterium and designated as Microbacterium festucae sp. nov. (type strain W1NT=ACCC 61807T=GDMCC 1.2966T=JCM 35339T) and Microbacterium nymphoidis sp. nov. (type strain W2RT=ACCC 61808T=GDMCC 1.2967T=JCM 35340T).

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Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil

Liu,

Yang,

Li

et al. 2024

Antonie van Leeuwenhoek

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Microbacterium stercoris sp. nov., an indole acetic acid-producing actinobacterium isolated from cow dung

Cited by 14 publications

References 45 publications

Characterization and evaluation of actinomycete from the Protaetia brevitarsis Larva Frass

Characterization and evaluation of actinomycete from the Protaetia brevitarsis Larva Frass

Microbacterium nymphoidis sp. nov. and Microbacterium festucae sp. nov., two novel species with high plant-promoting potential isolated from wetland plants in China

Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil

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