Microarray technology for yeast identification directly from positive blood cultures. A multicenter Italian experience

Abstract

The authors evaluated the performance of the MycArray™ Yeast ID (Myconostica Ltd, UK) assay in the identification of a total of 88 yeast isolates recovered in culture as compared to that obtained through routine methods. The turn-around time for species identification directly from cultures by the MycArray was 6 hours, much quicker than classical methods and all yeasts were correctly identified. In two cases a double identification including Saccharomyces cerevisiae was noted, but it was not confirmed by culture. The results show that MycArray Yeast ID can be a potential tool for rapid detection and identification of Candida species.

The Development of Diagnostics Tools and Techniques in the Isolation and Detection of Fungal Pathogens

“…Invasive fungal infections (IFIs) can be detected even at an early stage of development; this confers more sensitivity to the non-culture method [1][2][3][4][5][6][7][8][9][10][11]. The principle, just as in bacterial serology is the detection of antibodies or antigen in the serum.…”

“…This means a microarray is a potential tool for rapid detection and identification of yeast infections like; Candida species [10].…”

“…Also the choice of the buffer used might hinder the homogenization of cells. According to many fungal pathologists, CTAB or guanidinium thiocyanate based buffer is most appropriate [2][3][4][5][6][7][8][9][10]. In response to the need of attaining pure form of DNA, there are number of procedures that can assure the quality and quantity of DNA.…”

“…This brought a boost to the identification of human diseases. Of course their reliability, sensitivity and specificity put them before the traditional methods [1][2][3][4][5][6][7][8][9][10][11][12].…”

“…The Vitek MS system is an example of the newer version and it makes the method user friendly [2][3][4][5][6][7][8][9][10].…”