Microarray screening of differentially expressed genes after up-regulating miR-205 or down-regulating miR-141 in cervical cancer cells

Abstract

10 11 12 13 14 15 16 17 18 19 20 2 21 Microarray screening of differentially expressed genes after up-regulating miR-205 or 22 down-regulating miR-141 in cervical cancer cells 23 Abstract 24 Cervical cancer is one of the most common gynecological malignancies. However,studies 25 on the expression and molecular mechanism of miR-205 and miR-141 in CC are insufficient 26 recently. Expression profile microarray with 21329 Oligo DNA were used to detect the 27 expression of mRNAs in miR-205 up-regulated or miR-141 down-regulated HeLa and SiHa 28 cells and mRNAs in normal HeLa and SiHa cells. Gene Ontology (GO), Kyoto Encyclopedia 29 of Genes and Genomes (KEGG) pathway were performed to assess the potential pathways 30 of miR-205 in SiHa cell.Compared with normal HeLa cell, there were 38 differentially 31 expressed genes (DEGs) in miR-205 up-regulated HeLa cell. Nine were up-regulation genes 32 and 29 were down-regulation genes. There were 23 DEGs in miR-141 down-regulated HeLa 33 cell. One was up-regulated and 22 were down-regulated. Compared with normal SiHa cell, 34 there were 128 DEGs in miR-205 up-regulated SiHa cell. One hundred and three were up-35 regulation genes and 25 were down-regulation genes. There were 80 DEGs in miR-141 36 down-regulated SiHa cell. Forty two were up-regulation genes and 28 were down-regulation 37 genes. For miR-205 up-regulated SiHa cell, GO outcome showed that "ubiquitin-protein 38 ligase activity", "MAP kinase phosphatase activity", were the most enriched terms (P < 0.05). 39 And in KEGG analysis, "Cell cycle" was notably enriched, and Smad4 in this pathway was 40 up-regulated (P < 0.05). Expression profile microarray technology can effectively screen out 41 DEGs in cervical cancer cells after up-regulating miR-205 or down-regulating miR-141. 42 Which may enable us to understand the pathogenesis and lay an important foundation for 43 the prevention and treatment of cervical cancer. 44 Keywords: Gene expression profiling, cervical cancer, miRNA. 45 Introduction 3 46 Cervical cancer is one of the most common gynecological malignancies and the fourth 47 leading cause of cancer-related death worldwide. There were 527,600 new cases of cervical 48 cancer and 265,700 deaths worldwide in 2012.(1) It is the most common cancer of women in 49 many low-income countries.(2) Persistent infection of human papillomavirus (HPV) type 16 50 and 18 are the major risk factors for cervical cancer. In HPV high-risk cervical cancer cases, 51 HPV16 and 18 phenotypes account for 70%.(3) After HPV DNA integrated into the host DNA, 52 E6 and E7 genes are expressed. E6 induces the degradation of p53, stimulates telomerase 53 activity, and induces centrosome distortion and instability of the chromosome.(4) The binding 54 of E7 protein with human pRb and E2F transcription factor mediates cell transformation, 55 leading to the separation of pRb and E2F transcription factor and making cells enter S phase 56 of cell cycle early.(5) The occurrence of cervical cancer is a multi-factor, multi-stage and 57 multi-gene proce...