Microarray profiles reveal that circular RNA hsa_circ_0007385 functions as an oncogene in non-small cell lung cancer tumorigenesis

Jiang, Mingming; Mai, Zhitao; Wan, Shouhong; Chi, Yumin; Zhang, Xin; Sun, Baohua; Qin, Di

doi:10.1007/s00432-017-2576-2

Cited by 94 publications

(83 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification of new molecular mechanisms underlying the immune response has prompted many researchers to explore the involvement of these mechanisms in sustained inflammation in DKD. 20) Our study delineated a critical role of circRNA in glucose-induced inflammation and fibrosis in proximal tubular cells. Noncoding RNAs (ncRNAs) have attracted more attention than ever.…”

Section: Discussionmentioning

confidence: 75%

circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells <i>via</i> Pyroptosis

et al. 2020

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Diabetic kidney disease (DKD) is the leading cause of chronic kidney disease. Current therapies for DKD are insufficient. Therefore, there is an urgent need for identifying new therapies. An increasing number of micro RNAs (miRNAs) and long noncoding RNAs (lncRNAs) have been demonstrated to modulate the progression of diabetic kidney disease. Nevertheless, until now, there have been few reports evaluating the relevance of circular RNAs (circRNAs) in DKD. circRNAs have been reported to regulate the occurrence and development of multiple diseases. In this study, we intended to explore the circRNA expression profiles and determine the role of circRNA in DKD. We identified a series of dysregulated circRNAs in glucose-stressed HK-2 cells using circRNA microarray analysis. Among the candidate circRNAs, we found that circACTR2 was upregulated and may be involved in inflammation and pyroptosis. Knockdown of circACTR2 significantly decreased pyroptosis, interleukin (IL)-1β release and collagen IV and fibronectin production, indicating the effective regulation by circACTR2 of cell death and inflammation. Overall, our study identified a new circRNA, circACTR2, that regulates high glucose-induced pyroptosis, inflammation and fibrosis in proximal tubular cells. The present study preliminarily explores the role of circRNAs in pyroptosis of tubular cells, and provides novel insight into the pathogenesis of DKD and new therapeutic strategies.

show abstract

Section: Discussionmentioning

confidence: 75%

circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells <i>via</i> Pyroptosis

et al. 2020

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…For example, Zong et al showed that circRNA_102231 was increased and could act as a potential biomarker and therapeutic target for lung cancer patients [9]. Jiang et al [10] indicated that circular RNA hsa_circ_0007385 could function as an oncogene in nonsmall cell lung cancer tumorigenesis. Wan et al [11] indicated that circular RNA-ITCH suppressed lung cancer proliferation via inhibiting the Wnt/β-catenin pathway.…”

Section: Introductionmentioning

confidence: 99%

circFADS2 regulates lung cancer cells proliferation and invasion via acting as a sponge of miR-498

et al. 2018

View full text Add to dashboard Cite

CircRNAs could play critical functions in tumor progression. However, the expression and underlying mechanism of circRNAs in lung cancer progression remain poorly defined. In the present study, high-throughput microarray assay revealed that hsa_circRNA_100833 (identified as circFADS2) was markedly evaluated in lung cancer tissues, and it was further validated by qRT-PCR. High expression of circFADS2 was correlated with advanced TNM stage, lymph node metastasis, poor differentiation, and shorter overall survival of NSCLC patients. In vitro assays results showed that circFADS2 inhibition suppressed lung cancer cells proliferation and invasion ability. Bioinformatics analysis showed that miR-498 contained the complementary binding region of circFADS2, which was confirmed by Dual-luciferase reporter assay. In addition, the expression of miR-498 was down-regulated and negatively associated with circFADS2 expression in nonsmall cell lung cancer. Furthermore, rescue assays showed that miR-498 inhibitors abolished the effects of circFADS2 inhibition on lung cancer cells progression. Taken together, our findings indicated that circFADS2 was an effective tumor promoter in lung cancer progression, and its functions were performed by regulating the expression of miR-498. These data suggested that circFADS2 could act as a target for lung cancer treatment.

show abstract

“…22 A variety of circRNAs affect lung cancer invasion and migration by regulating related targets, such as hsa_circ_0007385, circ-BANP, and circPTK2. [23][24][25] In our study, the overexpression of circ-IGF1R in lung cancer cells inhibited cell migration and invasion and interfered with the ability of circ-IGF1R to promote cell invasion and migration. However, IGF1R displays potent antiapoptotic, prosurvival capacities and plays a key role in malignant transformation.…”

Section: Discussionmentioning

confidence: 55%

“…Tumor metastasis is a multistage process, which is regulated by several genes, including metastasis‐associated genes and metastasis‐suppressing genes . A variety of circRNAs affect lung cancer invasion and migration by regulating related targets, such as hsa_circ_0007385, circ‐BANP, and circPTK2 . In our study, the overexpression of circ‐IGF1R in lung cancer cells inhibited cell migration and invasion and interfered with the ability of circ‐IGF1R to promote cell invasion and migration.…”

Section: Discussionmentioning

confidence: 55%

Circ‐IGF1Rinhibits cell invasion and migration in non‐small cell lung cancer

Xiang

Chen

et al. 2020

Thoracic Cancer

View full text Add to dashboard Cite

Background Circular RNA (circRNA) is a novel molecular marker and target candidate that is closely associated with tumor invasion and migration. The mechanism of action of hsa_circ_0005035 (circ‐IGF1R) in non‐small cell lung cancer remains unclear. In this study, we aimed to study the mechanism of action of circ‐IGF1R in lung cancer. Methods We screened circ‐IGF1R, one of the most notable differential expressions, from the Gene Expression Omnibus database, GSE104854, for further research. The expression level of circ‐IGF1R was examined using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in five different lung cancer cell lines and 50 pairs of lung cancer and adjacent tissues. Wound‐healing and Transwell assays were used for verifying the biological function of circ‐IGF1R. The effect of overexpressing circ‐IGF1R on the transcriptome of whole lung cancer cells was explored in lung cancer cell lines using RNA‐seq. Results The expression level of circ‐IGF1R was notably lower in lung cancer tissues and lung cancer cell lines than in the adjacent normal tissues and cells (P < 0.0001). In addition, the expression level of circ‐IGF1R was associated with larger tumors (T2/T3/T4) and lymph node metastasis (N1/ N2/N3) (P < 0.05). The overexpression of circ‐IGF1R significantly inhibited the invasion and migration of the lung cancer cells. The potential network of circ‐IGF1R–miR‐1270–VANGL2 was preliminarily determined, and the expression patterns of miR‐1270 and VANGL2 were verified in lung cancer cell lines. Conclusion Circ‐IGF1R may inhibit lung cancer invasion and migration through a potential network of circ‐IGF1R–miR‐1270–VANGL2.

show abstract

Microarray profiles reveal that circular RNA hsa_circ_0007385 functions as an oncogene in non-small cell lung cancer tumorigenesis

Abstract: In summary, results suggest hsa_circ_0007385 plays a role in NSCLC tumorigenesis, providing a potential therapeutic target for NSCLC.

Cited by 94 publications

References 26 publications

circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells <i>via</i> Pyroptosis

circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells <i>via</i> Pyroptosis

circFADS2 regulates lung cancer cells proliferation and invasion via acting as a sponge of miR-498

Circ‐IGF1Rinhibits cell invasion and migration in non‐small cell lung cancer

Contact Info

Product

Resources

About