Microarray profile of B cells from Graves’ disease patients reveals biomarkers of proliferation

Jiang, Xuechao; Wang, Yonghui; Li, Xiaoying; He, Leqi; Yang, Qian; Wang, Wei; Liu, Jun; Zha, Bingbing

doi:10.1530/ec-20-0045

Cited by 7 publications

(4 citation statements)

References 50 publications

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“…B cells play an important role in autoimmune diseases, not only by producing antibodies to participate in autoimmune reactions but also by secreting cytokines and chemokines to recruit T cells (Luu, et al 2014). In addition, some marker proteins were expressed during the maturation of B cells, and were used to identify different subtypes; the dysfunction of B cells led to changes in autoimmune tolerance (Jiang, et al 2020;Kang, et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Infiltration of B cell may underlie the contrary sex- and age-related biological behavior of papillary thyroid carcinoma

Yan,

Sun,

et al. 2023

Preprint

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Background and purpose Sex and age play complex and contrary roles in the pathogenesis, lymph node metastasis (LNM), and prognosis of papillary thyroid carcinoma (PTC). This study aimed to explore the underlying mechanism of this phenomenon.Method The risk factors for LNM were explored by combining local data and the Surveillance, Epidemiology, and End Results (SEER) database of PTC. The gene expression information of 497 samples from The Cancer Genome Atlas Thyroid Cancer (TCGA-THCA) was used to explore the differentially expressed genes (DEGs) among different phenotypes. The single-cell RNA sequencing (scRNA-seq) data obtained from the Gene Expression Omnibus (GEO) database was used to explore the aimed gene expression in specific cell types. The flow cytometry was used to explore peripheral lymphocyte subsets in PTC.Results The clinical data showed that the younger age and the male sex were the risk factors for LNM (P < 0.05). In the bulk gene analysis, the age- and sex-related DEGs in contrary trends between different tissues and LNM state were two intersection genes:TCL1A and CR2. The analysis of scRNA-seq from the GEO database showed that the genes TCL1A and CR2 were mainly expressed in B cells, and the infiltration of B cells varied among different tissues. The peripheral blood lymphocyte subsets showed that the composition of circulating lymphocytes was different in different lymph node metastasis states and age levels.Conclusion The infiltration of TCL1A and CR2 related B cells for different ages and sexes might explain the contradictory biological behavior, including tumor pathogenesis, LNM, and prognosis of PTC.

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Section: Discussionmentioning

confidence: 99%

Infiltration of B cell may underlie the contrary sex- and age-related biological behavior of papillary thyroid carcinoma

Yan,

Sun,

et al. 2023

Preprint

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“…Yin et al performed a lncRNA microarray to identify AK021954, AB075506, and HMlincRNA1474 levels that were dysregulated in GD CD4 + T cells and might serve as novel biomarkers of GD [ 16 ]. Jiang et al found that n335641, n337845, and TCONS_00022357-XLOC_010919 may participate in the proliferation and survival of B cells in GD [ 21 ]. Yao et al focused on relapsed GD patients and found that three downregulated lncRNAs (NONHSAT093153.2, NONHSAT118924.2, and NONHSAT209004.1) were closely related to the recurrence of GD [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of lncRNA Expression Profile and the Potential Role of ENST00000604491 in Graves’ Disease

Liu

Zou

et al. 2022

Journal of Immunology Research

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Background. Graves’ disease (GD) is one of the most common autoimmune diseases worldwide and develops in 20 to 50 cases per 100,000 persons annually. Long noncoding RNAs (lncRNAs) are widely expressed in multiple human diseases and have pivotal functions in gene regulation. This study is aimed at determining the lncRNA profile in peripheral blood mononuclear cells (PBMCs) from GD patients and investigating the role of ENST00000604491 in GD. Methods. A total of 31 GD patients and 32 normal controls were enrolled in the study. Next-generation sequencing was performed to identify the dysregulated lncRNAs in the PBMCs from the 5 GD patients and 5 normal controls, and 26 GD patients and 27 controls were used to verify the selected lncRNAs. The relative expression of verified lncRNAs, forkhead box P1 (FOXP1), and IKAROS family zinc finger 3 (IKZF3) from these samples was detected by quantitative real-time PCR. The potential biomarker value was assessed by using receiver operating characteristic (ROC) curve analysis. Results. A total of 37,683 dysregulated expressed lncRNAs were indicated, of which 5 lncRNAs were significantly upregulated and 83 lncRNAs were remarkably downregulated in the GD patients compared with healthy subjects. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that abnormally expressed lncRNAs were mainly enriched in immune system-related signalling pathways. Among the selected lncRNAs, the relative expression of ENST00000604491 was significantly downregulated and negatively correlated with the serum levels of thyroid-stimulating hormone receptor antibodies (TRAb) in GD patients. Further studies confirmed that decreased FOXP1 expression was inversely correlated with serum TRAb levels in GD patients. Moreover, there was a notably positive correlation between ENST00000604491 expression and FOXP1 transcript levels in GD. The area under the ROC curve of ENST00000604491 was up to 0.74 (95% confidence interval: 0.60-0.87, p < 0.01 ), and the sensitivity and specificity were 53.85% and 88.89%, respectively. Conclusion. The present study identifies ENST00000604491 as a significantly attenuated lncRNA in GD patients, which may contribute to the pathogenesis of GD by regulating FOXP1 and represent a potential biomarker for GD.

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“…With the advancement in the depth and quality of transcriptome sequencing, an increasing number of distinguishably expressed lncRNAs has been illustrated in various disease. Several researches implicated lncRNAs in the development of various diseases, however, there are only scant data related to GD ( 27 , 28 , 29 ). In this study, we aimed to explore lncRNA and mRNA expression in GD CD4+T cells to provide new insight into the pathogenesis of GD.…”

Section: Discussionmentioning

confidence: 99%

lncRNA:mRNA expression profile in CD4+ T cells from patients with Graves’ disease

Yin

Zhou

et al. 2020

Endocrine Connections

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Graves’ disease (GD) is a common autoimmune disease that affects the thyroid gland. As a new class of modulators of gene expression, long non-coding RNAs (lncRNAs) have been reported to play a vital role in immune functions and in the development of autoimmunity and autoimmune disease. The aim of this study is to identify lncRNAs in CD4+ T cells as potential biomarkers of GD. lncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in GD CD4+ T cells compared with healthy control CD4+ T cells. Quantitative polymerase chain reaction (qPCR) was used to validate the results, and correlation analysis was used to analyze the relationship between these aberrantly expressed lncRNAs and clinical parameters. The microarray identified 164 lncRNAs and 93 mRNAs in GD CD4+ T cells differentially expressed compared to healthy control CD4+ T cells (fold change >2.0 and a P<0.05). Further analysis consistently showed that the expression of HMlincRNA1474 (P<0.01) and TCONS 00012608 (P<0.01) was suppressed, while the expression of AK021954 (P<0.01) and AB075506 (P<0.01) was upregulated from initial GD patients. In addition, their expression levels were recovered in euthyroid GD patients and GD patients in remission. Moreover, these four aberrantly expressed lncRNAs were correlated with GD clinical parameters. Moreover, the areas under the ROC curve were 0.8046, 0.7579, 0.8115 for AK021954, AB075506, HMlincRNA1474, respectively. The present work revealed that differentially expressed lncRNAs were associated with GD, which might serve as novel biomarkers of GD and potential targets for GD treatment.

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Microarray profile of B cells from Graves’ disease patients reveals biomarkers of proliferation

Cited by 7 publications

References 50 publications

Infiltration of B cell may underlie the contrary sex- and age-related biological behavior of papillary thyroid carcinoma

Infiltration of B cell may underlie the contrary sex- and age-related biological behavior of papillary thyroid carcinoma

Comprehensive Analysis of lncRNA Expression Profile and the Potential Role of ENST00000604491 in Graves’ Disease

lncRNA:mRNA expression profile in CD4+ T cells from patients with Graves’ disease

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