Microarray-guided evaluation of the frequency, B-cell origins, and selectivity of human glycan-binding antibodies reveals new insights and novel antibodies

Temme, J. Sebastian; Crainic, Jennifer A.; Walker, Laura M.; Yang, Weizhun; Tan, Zibin; Huang, Xuefei; Gildersleeve, Jeffrey C.

doi:10.1016/j.jbc.2022.102468

Cited by 4 publications

(5 citation statements)

References 75 publications

(134 reference statements)

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“…Isolated from a patient who recovered from an S. aureus infection, mAb F598 can protect mice against S. aureus infections 34 . The 32 PNAG pentasaccharide–BSA conjugates were immobilized onto a glycan microarray 35 . Following incubation of mAb F598 with the microarray and washing, the amount of antibody remaining bound was quantified with a fluorescent secondary antibody.…”

Section: Resultsmentioning

confidence: 99%

A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus

Tan,

Yang,

O’Brien

et al. 2024

Nat Commun

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Poly-β-(1–6)-N-acetylglucosamine (PNAG) is an important vaccine target, expressed on many pathogens. A critical hurdle in developing PNAG based vaccine is that the impacts of the number and the position of free amine vs N-acetylation on its antigenicity are not well understood. In this work, a divergent strategy is developed to synthesize a comprehensive library of 32 PNAG pentasaccharides. This library enables the identification of PNAG sequences with specific patterns of free amines as epitopes for vaccines against Staphylococcus aureus (S. aureus), an important human pathogen. Active vaccination with the conjugate of discovered PNAG epitope with mutant bacteriophage Qβ as a vaccine carrier as well as passive vaccination with diluted rabbit antisera provides mice with near complete protection against infections by S. aureus including methicillin-resistant S. aureus (MRSA). Thus, the comprehensive PNAG pentasaccharide library is an exciting tool to empower the design of next generation vaccines.

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Section: Resultsmentioning

confidence: 99%

A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus

Tan,

Yang,

O’Brien

et al. 2024

Nat Commun

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“…20 Since then, modern studies on antibodies to blood group antigens have been tremendously enhanced by the glycan microarray. 21 Note that some structures may fit into multiple groups (e.g., glycolipid and non-human glycan). Glycoglyph was used to produce glycan structures.…”

Section: Endogenous Anti-glycan Antibodiesmentioning

confidence: 99%

“…20 Since then, modern studies on antibodies to blood group antigens have been tremendously enhanced by the glycan microarray. 21…”

Section: Introductionmentioning

confidence: 99%

Serum antibody screening using glycan arrays

Marglous,

Brown,

Padler-Karavani

et al. 2024

Chem. Soc. Rev.

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“…With the advancement of robotic array spotting systems, several thousands of spots can be achieved simultaneously. Several DNA microarrays [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ], glycan microarrays [ 60 , 61 , 62 , 63 , 64 ], peptide microarrays [ 65 , 66 ], and protein microarrays [ 36 , 67 , 68 , 69 , 70 ] are fabricated using commercially available instruments, e.g., Smart Arrayer, OmniGrid contact microarrayer, Gene chip, Bio odyssey, BioRobotics MicroGRID II microarray printer, etc.…”

Section: Microarray Fabrication Biomolecule Immobilization and Detect...mentioning

confidence: 99%

“…The two carbohydrates heparin and fucoidan specifically recognized the spike protein of SARS-CoV-2. A glycan microarray consisting of 800 components identified 26 mAbs from a pool of 516 human mAbs [ 64 ]. The further analysis of these 26 mAbs provided insights on their cellular origins and binding specificities.…”

Section: Application Of Microarraysmentioning

confidence: 99%

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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Microarray-guided evaluation of the frequency, B-cell origins, and selectivity of human glycan-binding antibodies reveals new insights and novel antibodies

Cited by 4 publications

References 75 publications

A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus

A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus

Serum antibody screening using glycan arrays

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

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