Microarray Detection of Human Parainfluenzavirus 4 Infection Associated with Respiratory Failure in an Immunocompetent Adult

Chiu, Charles Y.; Rouskin, Silvi; Koshy, Anita A.; Urisman, Anatoly; Fischer, Kael F.; Yagi, Shigeo; Schnurr, David P.; Eckburg, Paul B.; Tompkins, Lucy S.; Blackburn, Brian G.; Merker, Jason D.; Patterson, Bruce K.; Ganem, Don; DeRisi, Joseph L.

doi:10.1086/507896

Cited by 87 publications

(89 citation statements)

References 21 publications

(22 reference statements)

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“…HPIV-2 was more sporadically detected. The initial pattern of HPIV-3 was endemic, but since 1978 it has changed to epidemic with outbreaks in late winter, spring or early summer, in the United States and Australia [3,6,7,17,26,29,30]. A small number of studies have reported an epidemiology of HPIV-4.…”

Section: Discussionmentioning

confidence: 99%

“…HPIV-1 to HPIV-3 are major causes of acute respiratory infections (ARI), including lower respiratory tract infections, in infants and children. These three types of HPIV have been more extensively investigated than HPIV-4 [2][3][4]. All subtypes can cause a full spectrum of respiratory illness, including upper respiratory infections, croup, bronchiolitis and pneumonia [1,5].…”

mentioning

confidence: 99%

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Parainfluenza virus infections in a tropical city: clinical and epidemiological aspects

Fé¹,

Monteiro²,

Moura³

2008

Braz J Infect Dis

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Human parainfluenza viruses (HPIV) are genetically and antigenically divided into types 1 to 4 [1]. HPIV-1 to HPIV-3 are major causes of acute respiratory infections (ARI), including lower respiratory tract infections, in infants and children. These three types of HPIV have been more extensively investigated than HPIV-4 [2-4]. All subtypes can cause a full spectrum of respiratory illness, including upper respiratory infections, croup, bronchiolitis and pneumonia [1,5]. Seasonal patterns of HPIV infections are distinct among the subtypes and geographic regions. In the USA, HPIV-2 can cause yearly outbreaks or biennial epidemics in cocirculation with HPIV-1 during the fall seasons, while HPIV-3 causes yearly spring and summer epidemics [5][6][7][8][9][10]. In England and Wales HPIV-3 causes yearly epidemics in the summer as shown [11]. In the northeast of England, a hospital-based study of the epidemiology of respiratory infections among pediatric inpatients over a six-year period showed that HPIV-1 and HPIV-2 epidemics occurred together, but only every 2 years [12]. In tropical countries, there have been fewer studies of HPIV and most of them do not demonstrate a seasonal pattern of HPIV infections [13,14].The purposes of the study were: (1) To determine the rate of HPIV infections among children attended at the Albert Sabin Children's Hospital (HIAS) over six consecutive years; (2) To describe the clinical and epidemiological characteristics of these infections; (3) To show the seasonal pattern of HPIV infections and its relation with the rainy season and with periods of activity of other respiratory viruses; and (4) To compare the clinical characteristics of parainfluenza infections with infections caused by respiratory syncytial virus (RSV), influenza and adenovirus. Material and Methods Site of StudyThis study was conducted at HIAS, a teaching hospital where care is provided to children from low income families living in Fortaleza and other cities in the state of Ceará. The city of Fortaleza is located at sea level, 4 o south of the equator and has a tropical climate characterized by two distinct seasons: the rainy season, that occurs in the first semester of each year, usually from January to June, and the dry season, during the rest of the year. The humidity is high (>70%), and there is little temperature variation throughout the year. During this study, the minimum and maximum daily temperatures in Fortaleza were 21.1 o C and 33.9 o C, respectively. Population of StudyThe patients included in this study were children and teenagers (0 to 16 years old) with acute respiratory symptoms. They were attended at the emergency department, outpatients' clinics and pediatric wards of HIAS from January 2000 to December 2006. Inclusion CriteriaPatients were included in the study if they had one or more of the following symptoms with or without fever (≥37.5 o C): cough, coryza, sore throat, earache, breathing difficulty, stridor and/or wheezing within seven days of onset. Epidemiological and Clinical DataThe guardians a...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Parainfluenza virus infections in a tropical city: clinical and epidemiological aspects

Fé¹,

Monteiro²,

Moura³

2008

Braz J Infect Dis

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“…The advent of broad-range molecular assays, including multiplex PCR and microarray systems, promises new insights into the epidemiology and pathogenesis of respiratory disease (8,9), given that a laboratory diagnosis is not routinely achieved for a substantial portion of respiratory specimens from symptomatic patients. We recently described the application of a multiplex PCR method for microbial surveillance wherein primers are attached to tags of varying mass that serve as digital signatures for their genetic targets.…”

Section: The Studymentioning

confidence: 99%

Global Distribution of Novel Rhinovirus Genotype

Briese

Renwick

Venter

et al. 2008

Emerg. Infect. Dis.

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“…To aid in the ability to detect viruses, a random, sequence-independent RT-rPCR protocol was evaluated to amplify viral RNA. 23 Other groups have reported using a similar method to randomly amplify virus for microarray detection from other sources such as nasal lavages, 12 endotracheal aspirates, 28 and homogenized mouse brains. 11 Using RT-rPCR amplicons, we were able to detect virus derived from cell culture but not from infected mosquitoes (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

Grubaugh¹,

Petz²,

Melanson³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. Highly multiplexed assays, such as microarrays, can benefit arbovirus surveillance by allowing researchers to screen for hundreds of targets at once. We evaluated amplification strategies and the practicality of a portable DNA microarray platform to analyze virus-infected mosquitoes. The prototype microarray design used here targeted the nonstructural protein 5, ribosomal RNA, and cytochrome b genes for the detection of flaviviruses, mosquitoes, and bloodmeals, respectively. We identified 13 of 14 flaviviruses from virus inoculated mosquitoes and cultured cells. Additionally, we differentiated between four mosquito genera and eight whole blood samples. The microarray platform was field evaluated in Thailand and successfully identified flaviviruses (Culex flavivirus, dengue-3, and Japanese encephalitis viruses), differentiated between mosquito genera (Aedes, Armigeres, Culex, and Mansonia), and detected mammalian bloodmeals (human and dog). We showed that the microarray platform and amplification strategies described here can be used to discern specific information on a wide variety of viruses and their vectors.

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Microarray Detection of Human Parainfluenzavirus 4 Infection Associated with Respiratory Failure in an Immunocompetent Adult

Cited by 87 publications

References 21 publications

Parainfluenza virus infections in a tropical city: clinical and epidemiological aspects

Parainfluenza virus infections in a tropical city: clinical and epidemiological aspects

Global Distribution of Novel Rhinovirus Genotype

Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

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