Microarray Chip Based Identification of a Mixed Infection of Bovine Herpesvirus 1 and Bovine Viral Diarrhea 2 From Indian Cattle

Ratta, Barkha; Yadav, Brijesh Singh; Pokhriyal, Mayank; Saxena, M.; Sharma, Bhaskar

doi:10.1007/s00284-013-0448-9

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…There is a high degree of conservation between HCMV gB and that of HSV and EBV. 91 , 92 , 95 We have a great deal to learn from other herpesvirus fields as HCMV structural biology has lagged behind. The HSV post-fusion crystal structure has been published for well over a decade, 96 and now have the first hints of a pre-fusion structure using cryo-electron tomography technology.…”

Section: Immunogen Structural Designmentioning

confidence: 99%

A new era in cytomegalovirus vaccinology: considerations for rational design of next-generation vaccines to prevent congenital cytomegalovirus infection

2018

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Human cytomegalovirus (HCMV), a member of the beta-herpesvirus family, is the most common cause of congenital infection worldwide as well as an important cause of morbidity in transplant recipients and immunosuppressed individuals. An estimated 1 in 150 infants are infected with HCMV at birth, which can result in lifelong, debilitating neurologic sequelae including microcephaly, sensorineural hearing loss, and cognitive impairment. Natural maternal immunity to HCMV decreases the frequency of reinfection and reduces risk of congenital transmission but does not completely protect against neonatal disease. Thus, a vaccine to reduce the incidence and severity of infant infection is a public health priority. A variety of candidate HCMV vaccine approaches have been tried previously, including live-attenuated viruses, glycoprotein subunit formulations, viral vectors, and single/bivalent DNA plasmids, but all have failed to reach target endpoints in clinical trials. Nevertheless, there is a great deal to be learned from the successes and failures of the HCMV vaccine field (both congenital and transplant-associated), as well as from vaccine development efforts for other herpesvirus pathogens including herpes simplex virus 1 and 2, varicella zoster virus, and Epstein–Barr virus. Here, we review those successes and failures, evaluating recent cutting-edge discoveries that have shaped the HCMV vaccine field and identifying topics of critical importance for future investigation. These considerations will inform rational design and evaluation of next-generation vaccines to prevent HCMV-associated congenital infection and disease.

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Section: Immunogen Structural Designmentioning

confidence: 99%

A new era in cytomegalovirus vaccinology: considerations for rational design of next-generation vaccines to prevent congenital cytomegalovirus infection

2018

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“…In one clinical sample (bovine nasal swab), microarray chip screening predicted presence of two viruses [BHV1 and bovine viral diarrhea 2 (BVD2)] ( Table 1 ). This is the first such report of mixed infection of BHV1 and BVD2 from India [ 17 ]. In one sample canine parvovirus 2 was identified and in another canine adeno virus-1 was identified ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…We have been working on developing broad range microarray chip for virus diagnosis in Indian livestock. We have earlier reported the identification of Newcastle disease virus in sheep [ 15 ] and mixed infection of bovine viral diarrhea virus subtype 2 and bovine herpes virus 1 [ 17 ] in cattle. We have also created an oligo-nucleotide probe catalogue for making customized microarray chip for virus diagnosis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Viral diagnosis in Indian livestock using customized microarray chips

et al. 2015

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Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified Bovine herpes virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2) in clinical samples. Microarray identified specific probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that customized application requires a detailed cost efficiency calculation.

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“…We have provided the list of cross reactive probes so that others can avoid using them in their probe dataset. This dataset was used to identify an unexpected case of Newcastle disease virus in sheep [8] and for identifying a mixed infection of Bovine viral diarrhea and Bovine herpesvirus in cattle [6]. The dataset can be accessed by user through the link https://dl.dropboxuser content.com/u/94060831/avpds/HOME.html.…”

Section: Resultsmentioning

confidence: 99%

Animal Viruses Probe Dataset (AVPDS) for Microarray-Based Diagnosis and Identification of Viruses

et al. 2013

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AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus specific probe has been divided into two fields namely virus name and probe sequence. Similarly conserved probes for virus genera table have genus, and subgroup within genus name and probe sequence. The subgroup within genus is artificially divided subgroups with no taxonomic significance and contains probes which identifies viruses in that specific subgroup of the genus. Using this dataset we have successfully diagnosed the first case of Newcastle disease virus in sheep and reported a mixed infection of Bovine viral diarrhea and Bovine herpesvirus in cattle. These dataset also contains probes which cross reacts across species experimentally though computationally they meet specifications. These probes have been marked. We hope that this dataset will be useful in microarray-based detection of viruses. The dataset can be accessed through the link https://dl.dropboxusercontent.com/u/94060831/avpds/HOME.html.

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Microarray Chip Based Identification of a Mixed Infection of Bovine Herpesvirus 1 and Bovine Viral Diarrhea 2 From Indian Cattle

Cited by 6 publications

References 24 publications

A new era in cytomegalovirus vaccinology: considerations for rational design of next-generation vaccines to prevent congenital cytomegalovirus infection

A new era in cytomegalovirus vaccinology: considerations for rational design of next-generation vaccines to prevent congenital cytomegalovirus infection

Viral diagnosis in Indian livestock using customized microarray chips

Animal Viruses Probe Dataset (AVPDS) for Microarray-Based Diagnosis and Identification of Viruses

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