Microarray-Based Technology for Glycomics Analysis

Gao, Jingqing; Ma, Lina; Liu, Dianjun

doi:10.2174/138620712798280808

Cited by 6 publications

(6 citation statements)

References 125 publications

(205 reference statements)

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“…At this point the application of lectin-microarrays has to be considered as well. The microarray technology was implemented in several variations using immobilized glycans or immobilized lectins, which were then tested against potential binding constituents, e.g., lectins or glycans [ 37 , 38 , 39 , 40 ]. Furthermore, lectin microarrays were also suggested for analysing the bacterial glycome [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms

Neu

Kuhlicke

2017

Microorganisms

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Microbial biofilm systems are defined as interface-associated microorganisms embedded into a self-produced matrix. The extracellular matrix represents a continuous challenge in terms of characterization and analysis. The tools applied in more detailed studies comprise extraction/chemical analysis, molecular characterization, and visualisation using various techniques. Imaging by laser microscopy became a standard tool for biofilm analysis, and, in combination with fluorescently labelled lectins, the glycoconjugates of the matrix can be assessed. By employing this approach a wide range of pure culture biofilms from different habitats were examined using the commercially available lectins. From the results, a binary barcode pattern of lectin binding can be generated. Furthermore, the results can be fine-tuned and transferred into a heat map according to signal intensity. The lectin barcode approach is suggested as a useful tool for investigating the biofilm matrix characteristics and dynamics at various levels, e.g. bacterial cell surfaces, adhesive footprints, individual microcolonies, and the gross biofilm or bio-aggregate. Hence fluorescence lectin bar-coding (FLBC) serves as a basis for a subsequent tailor-made fluorescence lectin-binding analysis (FLBA) of a particular biofilm. So far, the lectin approach represents the only tool for in situ characterization of the glycoconjugate makeup in biofilm systems. Furthermore, lectin staining lends itself to other fluorescence techniques in order to correlate it with cellular biofilm constituents in general and glycoconjugate producers in particular.

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Section: Discussionmentioning

confidence: 99%

Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms

Neu

Kuhlicke

2017

Microorganisms

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“…In the past few years, studies on carbohydrates and carbohydrate-protein interactions have been implemented by electrochemical methods, 8 surface plasmon resonance (SPR), 9 fluorescence spectroscopy, 10 UV-vis absorption spectroscopy, 11 and lectin microarray. 12,13 These technologies provide a better understanding of the structure and function of saccharides; moreover, there are a few studies on the fluorescent imaging of glycans by metabolic labelling, 14,15 but owing to the low resolution, the detailed distribution of glycans cannot be distinguished. Therefore, more efforts are necessary to reveal comprehensive information on carbohydrates at the molecular level under native conditions.…”

Section: Introductionmentioning

confidence: 99%

Revealing the carbohydrate pattern on a cell surface by super-resolution imaging

Chen

Gao

et al. 2015

Nanoscale

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Carbohydrates are involved in various physiological and pathological activities including cell adhesion, signal transduction and tumor invasion. The distribution of carbohydrates is the molecular basis of their multiple functions, but remains poorly understood. Here, we employed direct stochastic optical reconstruction microscopy (dSTORM) to visualize the pattern of N-acetylglucosamine (N-GlcNAc) on Vero cell membranes at the nanometer level of resolution. We found that N-GlcNAcs exist in irregular clusters on the apical membrane with an average cluster area of about 0.37 μm(2). Most of these N-GlcNAc clusters are co-localized with lipid rafts by dual-color dSTORM imaging, suggesting that carbohydrates are closely associated with lipid rafts as the functional domains. Our results demonstrate that super-resolution imaging is capable of characterizing the distribution of carbohydrates on the cellular surface at the molecular level.

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“…Used to screen for glycosidase specificity in environmental samples (Beloqui et al, 2012) Various Monolayer on gold, TOF For study of glycosyltransferase activity (Gao et al, 2012a) yields. To optimize the latter parameter, Unterieser et al (2011) have studied various parameters relevant to quantitation by both ESI and MALDI.…”

Section: Quantificationmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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Microarray-Based Technology for Glycomics Analysis

Cited by 6 publications

References 125 publications

Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms

Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms

Revealing the carbohydrate pattern on a cell surface by super-resolution imaging

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

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