Microarray based IgE detection in poly-sensitized allergic patients with suspected food allergy — an approach in four clinical cases

Vieira, T.; Lopes, Carla; Pereira, António; Araújo, Luís; Moreira, André; Delgado, Luís

doi:10.1016/j.aller.2011.03.013

Cited by 17 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, diverse profilin-sensitized patients only may react to a small number of profilin-containing allergen sources, probably due to a concomitance with other pollinosis [75], or sensitization to specific epitopes [76].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Effects of Polymorphism on Pollen Profilin Structural Functionality and the Generation of Conformational, T- and B-Cell Epitopes

2013

View full text Add to dashboard Cite

An extensive polymorphism analysis of pollen profilin, a fundamental regulator of the actin cytoskeleton dynamics, has been performed with a major focus in 3D-folding maintenance, changes in the 2-D structural elements, surface residues involved in ligands-profilin interactions and functionality, and the generation of conformational and lineal B- and T-cell epitopes variability.Our results revealed that while the general fold is conserved among profilins, substantial structural differences were found, particularly affecting the special distribution and length of different 2-D structural elements (i.e. cysteine residues), characteristic loops and coils, and numerous micro-heterogeneities present in fundamental residues directly involved in the interacting motifs, and to some extension these residues nearby to the ligand-interacting areas. Differential changes as result of polymorphism might contribute to generate functional variability among the plethora of profilin isoforms present in the olive pollen from different genetic background (olive cultivars), and between plant species, since biochemical interacting properties and binding affinities to natural ligands may be affected, particularly the interactions with different actin isoforms and phosphoinositides lipids species.Furthermore, conspicuous variability in lineal and conformational epitopes was found between profilins belonging to the same olive cultivar, and among different cultivars as direct implication of sequences polymorphism. The variability of the residues taking part of IgE-binding epitopes might be the final responsible of the differences in cross-reactivity among olive pollen cultivars, among pollen and plant-derived food allergens, as well as between distantly related pollen species, leading to a variable range of allergy reactions among atopic patients. Identification and analysis of commonly shared and specific epitopes in profilin isoforms is essential to gain knowledge about the interacting surface of these epitopes, and for a better understanding of immune responses, helping design and development of rational and effective immunotherapy strategies for the treatment of allergy diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of the Effects of Polymorphism on Pollen Profilin Structural Functionality and the Generation of Conformational, T- and B-Cell Epitopes

2013

View full text Add to dashboard Cite

show abstract

“… Serum‐specific IgE measurements should be considered when SPTs are discordant with the medical history or contraindicated, or as an alternative to SPT to quantify specific IgE to naïve allergens and/or their purified components. The component‐resolved diagnosis, based on the pure allergen molecules (microarray or recombinant allergen–specific IgE detection), may be helpful in the future for diagnosis in patients with multiple sensitizations . Total serum IgE is no longer considered indispensable for diagnosis, because normal values do not exclude a diagnosis of allergy. Alternatively, the multiple allergen screening test may be useful . Conjunctival provocation test (CPT)/Conjunctival allergen challenge (CAC) can determine or confirm which allergen(s) triggers the ocular symptoms .…”

Section: Investigation Of Ocular Allergymentioning

confidence: 99%

Ocular allergy: recognizing and diagnosing hypersensitivity disorders of the ocular surface

Leonardi

Bogacka

Fauquert

et al. 2012

Allergy

Self Cite

191

255

View full text Add to dashboard Cite

Ocular allergy includes several clinically different conditions that can be considered as hypersensitivity disorders of the ocular surface. The classification of these conditions is complex, and their epidemiology has not been adequately studied because of the lack of unequivocal nomenclature. Ocular allergy symptoms are often, but not always, associated with other allergic manifestations, mostly rhinitis. However, specific ocular allergic diseases need to be recognized and managed by a team that includes both an ophthalmologist and an allergist. The diagnosis of ocular allergy is usually based on clinical history and signs and symptoms, with the support of in vivo and in vitro tests when the identification of the specific allergic sensitization is required for patient management. The aims of this Task Force Report are (i) to unify the nomenclature and classification of ocular allergy, by combining the ophthalmology and allergy Allergic Rhinitis and its Impact on Asthma criteria; (ii) to describe current methods of diagnosis; (iii) to summarize the therapeutic options for the management of ocular allergic inflammation. Ocular allergy is a localized allergic condition that is observed as the only or dominant presentation of an allergic sensitiza-tion, or is associated with rhinitis. It is not a single clinical entity, but includes several conditions with different pathogen-esis, hypersensitivity mechanisms, diagnostic criteria, and management. Ocular allergies are encountered daily in the phy-sician's office. Approximately 15-20% of the world population is affected by some form of allergic disease; ocular symptoms are estimated to be present in 40-60% of allergic patients (1) and contribute significantly to poor quality of life (2-4). Most of the available prevalence data encompass both ocular and nasal symptoms, making it impossible to separate ocular allergy from allergic rhinitis. Moreover, the frequently confusing nomenclature makes estimations of prevalence difficult. The purpose of this position paper is to unify the nomenclature and classification of ocular allergies, in order to facilitate the exchange of information and knowledge on diagnosis and management between allergists and ophthalmologists. The existing evidence for treatment options was evaluated using the SIGN criteria (5). Classification and nomenclature The ocular allergy nomenclature is based either on clinical signs and symptoms (Table 1) or on pathophysiology, according to the different hypersensitivity mechanisms introduced by Gell and Coombs. In 2001, the European Academy of Allergy and Clinical Immunology (EAACI)

show abstract

“…The cross-reactive components were ordered into families [26]. The allergen- or component-associated clinical pictures, such as the pollen-food syndromes were also considered [19,27,28]. Finally, the rules for the classification of the phenotypes [29] and strategies for the prescription of allergen specific immunotherapies [3-6,30-32] were also implemented.…”

Section: Methodsmentioning

confidence: 99%

Allergenius, an expert system for the interpretation of allergen microarray results

Melioli

Spenser

Reggiardo

et al. 2014

World Allergy Organization Journal

View full text Add to dashboard Cite

BackgroundAn in vitro procedure based on a microarray containing many different allergen components has recently been introduced for use in allergy diagnosis. Recombinant and highly purified allergens belonging to different allergenic sources (inhalants, food, latex and hymenoptera) are present in the array. These components can either be genuine or cross-reactive, resistant or susceptible to heat and low pH, and innocuous or potentially dangerous. A large number of complex and heterogeneous relationships among these components has emerged, such that sometimes these interactions cannot be effectively managed by the allergist. In the 1960s, specialized languages and environments were developed to support the replacement of human experts with dedicated decision-making information systems. Currently, expert systems (ES) are advanced informatics tools that are widely used in medicine, engineering, finance and trading.MethodsWe developed an ES, named Allergenius ®, to support the interpretation of allergy tests based on microarray technology (ImmunoCAP ISAC ®). The ES was implemented using Flex, a LPA Win-Prolog shell. Rules representing the knowledge base (KB) were derived from the literature and specialized databases. The input data included the patient’s ID and disease(s), the results of either a skin prick test or specific IgE assays and ISAC results. The output was a medical report.ResultsThe ES was first validated using artificial and real life cases and passed all in silico validations. Then, the opinions of allergists with experience in molecular diagnostics were compared with the ES reports. The Allergenius reports included all of the allergists’ opinions and considerations, as well as any additional information.ConclusionsAllergenius is a trustable ES dedicated to molecular tests for allergy. In the present version, it provides a powerful method to understand ISAC results and to obtain a comprehensive interpretation of the patient’s IgE profiling.

show abstract

Microarray based IgE detection in poly-sensitized allergic patients with suspected food allergy — an approach in four clinical cases

Abstract: The possibility to carry out multiple sIgE measurements with single protein allergens, in particular with the microarray technique, is a useful, simple and non-invasive diagnostic tool in complex poly-sensitized allergic patients.

Cited by 17 publications

References 31 publications

Analysis of the Effects of Polymorphism on Pollen Profilin Structural Functionality and the Generation of Conformational, T- and B-Cell Epitopes

Analysis of the Effects of Polymorphism on Pollen Profilin Structural Functionality and the Generation of Conformational, T- and B-Cell Epitopes

Ocular allergy: recognizing and diagnosing hypersensitivity disorders of the ocular surface

Allergenius, an expert system for the interpretation of allergen microarray results

Contact Info

Product

Resources

About