Microarray-based gene expression analysis during retinal maturation of albino rats

Ben‐Shlomo, Gil; Ofri, Ron; Bandah, D.; Rosner, Mordechai; Sharon, Dror

doi:10.1007/s00417-008-0772-0

Cited by 3 publications

(3 citation statements)

References 38 publications

(44 reference statements)

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“…Interestingly, ATCAY , a gene known to cause cerebellar ataxia got activated with 23 treatment 34 ( Figure 2o ). Similarly, 24 activated the sensory system associated SYTL1 4 and 25 activated digestive system associated MYO7A 35 and RBFOX3 ( Figure 2p–r ). Control studies carried out by treating HDFs with SAHA-alone did not activate any of these therapeutically important genes ( Figure 2 a–r , Bars SAHA).…”

Section: Resultsmentioning

confidence: 88%

“…Modern experimental techniques assist us in recognizing genes of therapeutic significance 1 . Recently, there has been an exponential increase in the number of genes that gets classified as the potential therapeutic targets 2 3 4 5 . Increasing correlation between aberrant transcription patterns and diseases further stimulate the need for effectors capable of modulating these faulty gene(s)/transcription factors 6 7 .…”

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confidence: 99%

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Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts

Pandian

Taniguchi

Syed

et al. 2014

Sci Rep

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The influential role of the epigenome in orchestrating genome-wide transcriptional activation instigates the demand for the artificial genetic switches with distinct DNA sequence recognition. Recently, we developed a novel class of epigenetically active small molecules called SAHA-PIPs by conjugating selective DNA binding pyrrole-imidazole polyamides (PIPs) with the histone deacetylase inhibitor SAHA. Screening studies revealed that certain SAHA-PIPs trigger targeted transcriptional activation of pluripotency and germ cell genes in mouse and human fibroblasts, respectively. Through microarray studies and functional analysis, here we demonstrate for the first time the remarkable ability of thirty-two different SAHA-PIPs to trigger the transcriptional activation of exclusive clusters of genes and noncoding RNAs. QRT-PCR validated the microarray data, and some SAHA-PIPs activated therapeutically significant genes like KSR2. Based on the aforementioned results, we propose the potential use of SAHA-PIPs as reagents capable of targeted transcriptional activation.

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Section: Resultsmentioning

confidence: 88%

mentioning

confidence: 99%

Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts

Pandian

Taniguchi

Syed

et al. 2014

Sci Rep

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“…Previous authors have also sought to determine the retinal gene expression profile as a function of age in both macular and peripheral retina using smaller sample sizes (Yoshida et al, 2002; Hornan et al, 2007; Ben-Shlomo et al, 2008). Yoshida et al developed gene expression profiles of young and elderly human retinas using microarray slides containing 2400 human genes that were primarily neuronal.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aging and Anatomic Location on Gene Expression in Human Retina

Cai¹,

Fields²,

Hoshino³

et al. 2012

Front. Ag. Neurosci

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Objective: To determine the effects of age and topographic location on gene expression in human neural retina. Methods: Macular and peripheral neural retina RNA was isolated from human donor eyes for DNA microarray and quantitative RT-PCR analyses. Results: Total RNA integrity from human donors was preserved. Hierarchical clustering analysis demonstrates that the gene expression profiles of young, old, macula, and peripheral retina cluster into four distinct groups. Genes which are highly expressed in macular, peripheral, young, or old retina were identified, including inhibitors of Wnt Signaling Pathway (DKK1, FZD10, and SFRP2) which are preferably expressed in the periphery. Conclusion: The transcriptome of the human retina is affected by age and topographic location. Wnt pathway inhibitors in the periphery may maintain peripheral retinal cells in an undifferentiated state. Understanding the effects of age and topographic location on gene expression may lead to the development of new therapeutic interventions for age-related eye diseases.

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Current World Literature

2009

Current Opinion in Ophthalmology

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Microarray-based gene expression analysis during retinal maturation of albino rats

Abstract: These data, taken together with our previously reported electrophysiological data, contribute to our understanding of the retinal maturation processes of this widely-used animal model.

Cited by 3 publications

References 38 publications

Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts

Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts

Effects of Aging and Anatomic Location on Gene Expression in Human Retina

Current World Literature

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