Microarray analysis of the rat lacrimal gland following the loss of parasympathetic control of secretion

Nguyen, Doan; Toshida, Hiroshi; Schurr, Jill R.; Beuerman, Roger W.

doi:10.1152/physiolgenomics.00011.2004

Cited by 29 publications

(28 citation statements)

References 80 publications

(96 reference statements)

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“…In the absence of a systemic immune disorder, primary LG dysfunction may be manifested by alterations to the neural and endocrine mechanisms, which alter the secretory function of the LG (Sullivan, 1999). Based on these studies, as well as our previous DNA microarray analysis studies showing an increase in the expression of pro-inflammatory and proapoptotic mediators after GSPN sectioning (Nguyen et al, 2004), it is plausible that alteration of muscarinic parasympathetic input and the LG secretory apparatus may be an early initiation factor for activating an immune response in the LG, but further investigation and verification are warranted.…”

Section: Introductionmentioning

confidence: 73%

“…Male Long-Evans rats (200-250 g) were purchased from Harlan Laboratories (Indianapolis, IN) and controlled pre-ganglionic parasympathetic denervation was carried out as previously described (Nguyen et al, 2004). All animal studies were carried out in accordance with the ARVO Statement on the Use of Animals in Research in Vision and Ophthalmology and with approval from the Louisiana State University Health Sciences Center Institutional Animal Care and Use Committee (IACUC).…”

Section: Pre-ganglionic Parasympathetic Denervationmentioning

confidence: 99%

“…Total RNA from the Ctla and Px LG (n = 3) was extracted using the RNeasy Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer's instructions and as previously described (Nguyen et al, 2004). RNA quality was determined by spectrophotometry and all RNA samples had an A 260 /A 280 ratio of 1.9 or greater.…”

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…Genes of interest that were found to be differentially expressed by DNA microarray analysis (Nguyen et al, 2004) were selected for validation by real-time PCR. Genespecific primers and TaqMan probes were designed using Beacon Designer 2.0 (Premier Biosoft International, Palo Alto, CA) and purchased from the LSU Health Sciences Center Core Laboratories and Integrated DNA Technologies (Coralville, IA).…”

Section: Primer Design and Real-time Pcrmentioning

confidence: 99%

“…The LG from the sham-denervated animals were similar to the Ctla LG. Subsequent gene expression analysis of the Ctla and Px LG by DNA microarray found increased expression of pro-inflammatory, pro-apoptotic, and immune regulatory genes in the Px LG (Nguyen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Loss of parasympathetic innervation leads to sustained expression of pro-inflammatory genes in the rat lacrimal gland

Nguyen

Vadlamudi

Toshida

et al. 2006

Autonomic Neuroscience

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It has been shown that removal of parasympathetic innervation to the lacrimal gland (LG) leads to rapid reduction in tear flow. Additionally, removal of the neural input resulted in disorganization of LG structure and changes in the expression of genes associated with the secretory pathway and inflammation. The goal of this study was to investigate the change in pro-inflammatory and proapoptotic gene expression in the rat LG following parasympathetic denervation. Male Long-Evans rats underwent unilateral sectioning of the greater superficial petrosal nerve and were sacrificed 7 days or 2.5 months later. cDNA was synthesized from LG RNA from the contralateral control (Ctla) and parasympathectomized (Px) glands and comparative real-time PCR was performed. Mean threshold cycles (MC T ) for the Ctla and Px LG genes were normalized to 18S rRNA MC T values, and the relative fold change was calculated for each gene using the method. The expression of nuclear factor kappa B1, caspase 1, eotaxin, leukocyte antigen MRC-OX44, allograft inflammatory factor-1, MHC class II molecules RT.1B and RT.1D, IgG receptor FcRn, and macrophage metalloelastase was increased and remained elevated in the Px LG, compared with the Ctla LG. Increased expression of the initiator of apoptosis gene, caspase 2, was confirmed, but expression of the executor gene, caspase 6, was not elevated in the Px LG. Reduced expression of genes associated with post-translational protein processing-furin convertase, protein disulfide isomerase, and UDP-gal transporter isozyme 1-was noted in the Px LG. No significant changes in the expression of genes associated with lysosomal and non-lysosomal-mediated protein degradation were found. Removal of parasympathetic input may lead to decreased capacity for protein synthesis and elevated immune responses in the Px LG. These changes occur without increases in expression of the muscarinic acetylcholine receptor subtype 3, and may suggest the early changes in LG acinar cells and the pathophysiology of autoimmune responses.

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Section: Introductionmentioning

confidence: 73%

Section: Pre-ganglionic Parasympathetic Denervationmentioning

confidence: 99%

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Section: Primer Design and Real-time Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Loss of parasympathetic innervation leads to sustained expression of pro-inflammatory genes in the rat lacrimal gland

Nguyen

Vadlamudi

Toshida

et al. 2006

Autonomic Neuroscience

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Melanocortin 5 receptor signaling pathway in health and disease

Guan

Zhou

et al. 2020

Cell. Mol. Life Sci.

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Melanocortin hormone system plays a key role in maintaining the homeostasis of our body via their neuro-immune-endocrine activities and regulates a diverse array of physiological functions, including melanogenesis, inflammation, immunomodulation, adrenocortical steroidogenesis, hemodynamics, natriuresis, energy homeostasis, sexual function, and exocrine secretion. The pathobiologic actions of all melanocortins are conveyed by melanocortin receptors. As the last melanocortin receptor to be cloned and characterized, melanocortin receptor 5 (MC5R) is widely expressed in both central nervous system and a number of peripheral organ systems in man. However, the exact effect of the MC5R mediated melanocortinergic signaling remains largely uncertain. Owing to the recent advances in developing highly selective peptidomimetic agonists and antagonists of MC5R and also to studies in MC5R knockout animals, our understanding of MC5R pathobiology has been greatly expanded and strengthened. Evidence suggests that MC5R plays a key role in governing immune reaction and inflammatory response, and is pivotal for the regulation of sexual behavior, thermoregulation, and exocrine secretion, like sebogenesis, lacrimal secretion and release of sex pheromones. As such, recent translational efforts have focused on developing novel sebum-suppressive therapies for seborrhoea and acne vulgaris based on antagonizing MC5R. Conversely, selective MC5R agonists have demonstrated promising beneficial effects in immune-mediated diseases, metabolic endocrinopathies and other disease conditions, such as glomerular diseases and dry eyes, skin and mouth. Thus, MC5R-mediated signaling is essential for health. Therapeutic targeting of MC5R represents a promising and pragmatic therapeutic strategy for diverse diseases. This review article delineates the Terms of use and reuse: academic research for non-commercial purposes, see here for full terms. https://www.springer.com/aamterms-v1

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Use of bioanalyzer electropherograms for quality control and target evaluation in microarray expression profiling studies of ocular tissues

Harrington

Winther

Garred

2009

j ocul biol dis inform

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Expression profiling with DNA microarrays has been used to examine the transcriptome of a wide spectrum of vertebrate cells and tissues. The sensitivity and accuracy of the data generated is dependent on the quality and composition of the input RNA. In this report, we examine the quality and array performance of over 200 total RNA samples extracted from ocular tissues and cells that have been processed in a microarray core laboratory over a 7-year period. Total RNA integrity and cRNA target size distribution were assessed using the 2100 Bioanalyzer. We present Affymetrix GeneChip array performance metrics for different ocular samples processed according to a standard microarray assay workflow including several quality control checkpoints. Our review of ocular sample performance in the microarray assay demonstrates the value of considering tissue-specific characteristics in evaluating array data. Specifically, we show that Bioanalyzer electropherograms reveal highly abundant mRNAs in lacrimal gland targets that are correlated with variation in array assay performance. Our results provide useful benchmarks for other gene expression studies of ocular systems.

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Microarray analysis of the rat lacrimal gland following the loss of parasympathetic control of secretion

Cited by 29 publications

References 80 publications

Loss of parasympathetic innervation leads to sustained expression of pro-inflammatory genes in the rat lacrimal gland

Loss of parasympathetic innervation leads to sustained expression of pro-inflammatory genes in the rat lacrimal gland

Melanocortin 5 receptor signaling pathway in health and disease

Use of bioanalyzer electropherograms for quality control and target evaluation in microarray expression profiling studies of ocular tissues

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