Microarray Analysis of the Expression Profile of Long Non-Coding RNAs Indicates lncRNA RP11-263F15.1 as a Biomarker for Diagnosis and Prognostic Prediction of Pancreatic Ductal Adenocarcinoma

Huang, Xiaolun; Ta, Na; Zhang, Yunshuo; Yuan-fu, Gao; Hu, Ronglei; Deng, Lulu; Zhang, Bingbing; Jiang, Hui; Zheng, Jianming

doi:10.7150/jca.18073

Cited by 10 publications

(6 citation statements)

References 34 publications

(29 reference statements)

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“…Several studies have demonstrated that lncRNAs are major regulators of gene expression and essential in a variety of pathological procedures, including cancer, cardiovascular diseases and neuronal disorders (22)(23)(24)(25). In addition, the expression profiles of lncRNAs are altered in cancer (26,27). The role and therapeutic potential of cancer-related lncRNAs have become a research hotspot in recent years.…”

Section: Discussionmentioning

confidence: 99%

Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143

Chen

et al. 2021

Oncol Lett

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Osteosarcoma is a malignant bone tumor, which has a high incidence in children and adolescents. However, the pathogenesis of osteosarcoma remains unclear. Long noncoding RNA (lncRNA) is a new potential therapeutic target and diagnostic biomarker for osteosarcoma. Hence, the present study aimed to explore the effect of lncRNA colon cancer-associated transcript (CCAT2) on osteosarcoma and its potential underlying mechanisms. For this purpose, the proliferation of osteosarcoma cells was measured using the CCK-8 assay. The scratch-wound and cell invasion assays were used to determine the migration and invasion of osteosarcoma cells, respectively. LncRNA CCAT2 and microRNA (miR)-143 binding sites were identified by the dual-luciferase reporter assay. RNA and protein expression levels were detected by reverse-transcription quantitative PCR and western blotting, respectively. Downregulation of lncRNA CCAT2 inhibited the proliferation, migration, and invasion of osteosarcoma cells. The findings also revealed that miR-143 bound directly to lncRNA CCAT2. The expression of miR-143 was upregulated by the knockdown of lncRNA CCAT2. Downregulation of the FOS-like antigen 2 was also observed after knockdown of lncRNA CCAT2. The function of lncRNA CCAT2 in osteosarcoma cells was attenuated by co-transfection with anti-miR-143 oligodeoxyribonucleotide. In conclusion, downregulation of lncRNA CCAT2 inhibited the proliferation and metastasis of osteosarcoma cells by targeting miR-143. lncRNA CCAT2 was identified as a potential target for osteosarcoma treatment.

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Section: Discussionmentioning

confidence: 99%

Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143

Chen

et al. 2021

Oncol Lett

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“…A panel contains 8 circulating lncRNAs might be beneficial for early prediction of pancreatic cancer . Microarray analysis revealed that a single lncRNA (RP11‐263F15.1) might be useful in the diagnosis and prognostic of PDAC . Due to a large number of lncRNAs in the genome, we believed that some important lncRNAs in PDAC development are still undetermined, and more comprehensive studies should be taken to truly discover novel lncRNAs.…”

Section: Introductionmentioning

confidence: 99%

“…28 Microarray analysis revealed that a single lncRNA (RP11-263F15.1) might be useful in the diagnosis and prognostic of PDAC. 29 Due to a large number of lncRNAs in the genome, we believed that some important lncRNAs in PDAC development are still undetermined, and more comprehensive studies should be taken to truly discover novel lncRNAs. Here, by mining the cancer genome atlas (TCGA) data set, we uncovered 8 novel lncRNAs that might be important in PDAC.…”

Section: Introductionmentioning

confidence: 99%

Orchestrating a biomarker panel with lncRNAs and mRNAs for predicting survival in pancreatic ductal adenocarcinoma

Wei

Ming

et al. 2018

J of Cellular Biochemistry

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The low survival of patients with pancreatic ductal adenocarcinoma (PDAC) makes the treatment of this disease one of the most challenging task in modern medicine. Here, by mining a large-scale cancer genome atlas data set of pancreatic cancer tissues, we identified 21 long noncoding RNAs (lncRNAs) that significantly associated with overall survival in patients with PDAC (P < .01). Further analysis revealed that 8 lncRNAs turned out to be independently correlated with patients' overall survival, and the risk score could be calculated based on their expression. To obtain a better predicting power, we integrated lncRNA data with a total of 410 differently expressed messenger RNAs (mRNAs) screened from PDAC and normal tissues in gene expression omnibus (GEO) database. The integration resulted in a much better panel including 8 lncRNAs (RP3.470B24.5, CTA.941F9.9, RP11.557H15.3, LINC00960, AP000479.1, LINC00635, LINC00636, and AC073133.1) and 8 mRNAs (DHRS9, ONECUT1, OR8D4, MT1M, TCN1, MMP9, DPYSL3, and TTN) to predict prognosis. A functional evaluation showed that these lncRNAs might play roles in pancreatic secretion, cell adhesion, and proteolysis. Using normal and pancreatic cancer cell lines, we confirmed that a majority of identified lncRNAs and mRNAs showed altered expressions in pancreatic cancer cells. Especially, LINC01589, LINC00960, TCN1, and MT1M showed a profoundly increased expression in pancreatic cancer cells, which suggests their potentially important role in pancreatic cancer. The results of our work indicate that lncRNAs have vital roles in PADC and provide new insights to integrate multiple kinds of markers in clinical practices.

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“…However RT-qPCR has also some limitations such as: the amplicon size cannot be detected; maximum of four lncRNAs can be detected simultaneously; and non-specific binding can occur if SYBR ® green is used, among others [ 93 ] . Another technique commonly used to identify lncRNAs are microarrays [ 94 , 95 ] , which consist of a library of DNA specific sequences (known as probes) immobilized in a grid. The isolated mRNA is reversed transcribed into cDNA incorporating fluorescent probes and hybridized to the microarray.…”

Section: Technologies For the Isolation And Detection Of Exosomal Lon...mentioning

confidence: 99%

Exosomal lncRNAs: the newest promising liquid biopsy

Gabriel¹,

Heymann²

2019

CDR

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LncRNAs are defined as RNA transcripts greater than 200 nucleotides in length that have no or limited protein-coding potential. Basal expression of lncRNAs appeared important for various homeostatic processes, like gene imprinting cell differentiation and organogenesis. Moreover, it has been demonstrated that lncRNAs play an important role in tumorigenesis and metastasis. Some lncRNAs were stably detected in exosomes, which are widely found in body fluids. Several studies validated the use of exosomal lncRNAs as minimally invasive diagnostic and prognostic markers in several types of cancers. In addition, exosomal lncRNAs have been associated with drug resistance of tumor cells, suggesting a clinical application in cancer-targeted therapy. Despite the recent increase of studies on exosomal lncRNAs, their clinical significance in cancer diagnosis, prognosis and treatment needs to be fully explored. The methodologies for their detection with high purity and accuracy must be also improved in order to implement their use in clinical routine. This review aims to summarize the main recent technologies available for the isolation of exosomal lncRNAs, their status as a liquid biopsy as well as their future perspectives.

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Microarray Analysis of the Expression Profile of Long Non-Coding RNAs Indicates lncRNA RP11-263F15.1 as a Biomarker for Diagnosis and Prognostic Prediction of Pancreatic Ductal Adenocarcinoma

Cited by 10 publications

References 34 publications

Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143

Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143

Orchestrating a biomarker panel with lncRNAs and mRNAs for predicting survival in pancreatic ductal adenocarcinoma

Exosomal lncRNAs: the newest promising liquid biopsy

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