Microarray Analysis of Neointima

Levy, Shawn; Muldowney, James A.S.

doi:10.1161/01.atv.0000042201.18694.0d

ATVB

2002

DOI: 10.1161/01.atv.0000042201.18694.0d

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Microarray Analysis of Neointima

Shawn Levy

James A.S. Muldowney

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Cited by 2 publications

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References 20 publications

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“…High-throughput microarray analysis of gene expression provides a way to understand the complexity of the underlying molecular pathology of cardiovascular disease and to identify suitable target genes for therapy [Levy and Muldowney, 2002]. A number of microarray studies have examined major cardiovascular diseases including microscopic specimens of human stent-induced neointima samples [Zohlnhofer et al, 2001a,b;Kotani et al, 2003].…”

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confidence: 99%

Temporal evolution of gene expression in rat carotid artery following balloon angioplasty

Zhang

Nelson

et al. 2006

J of Cellular Biochemistry

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The success of vascular intervention including angioplasty, stenting, and arterial bypass remains limited by negative remodeling resulted in lumen restenosis. This study was to characterize the global transcription profile reflecting concurrent events along arterial remodeling and neointima formation in a rat carotid artery balloon-injury model. Expression profiling of injured and control common carotid arteries on days 4, 7, 14 post-injury that mark the major pathohistological progression stages of neointimal formation were recorded on high-density oligonucleotide arrays. A subset of genes from microarray-based data was further studied using quantitative real time RT-PCR and in situ hybridization with sequential arterial samples from days 1 to 28 post-injury. The gene-encoded proteins were validated with Western blot. Besides temporal induction of a large cluster of genes over-represented by cell proliferation and macromolecule metabolism gene ontology categories, a fast-evolving inflammation could be demonstrated by the induction of Tgfb and other anti-inflammatory genes (e.g., C1qtnf3 (C1q and tumor necrosis factor related protein 3 (predicted))) and a shift from type 1 to 2 helper T cell response. The most significant signature of the induced neointimal profile is enrichment of genes functionally related to angiogenesis and extracellular matrix (ECM) remodeling (e.g., Spp1 (secreted phosphoprotein 1), CD44 (CD44 antigen), and Cxcl12 (chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)). Some of the genes represent stress-responsive mesenchymal stromal cell cytokines. This study highlighted mesenchymal stromal cell cytokines-driven inflammatory extracellular matrix remodeling, as target processes for potential clinical therapeutic intervention.

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confidence: 99%

Temporal evolution of gene expression in rat carotid artery following balloon angioplasty

Zhang

Nelson

et al. 2006

J of Cellular Biochemistry

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Temporal gene expression following prosthetic arterial grafting1

Willis

Kalish

et al. 2004

Journal of Surgical Research

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Microarray Analysis of Neointima

Cited by 2 publications

References 20 publications

Temporal evolution of gene expression in rat carotid artery following balloon angioplasty

Temporal evolution of gene expression in rat carotid artery following balloon angioplasty

Temporal gene expression following prosthetic arterial grafting1

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