Microarray Analysis of Juvenile Hormone Response in<i>Drosophila melanogaster</i>S2 cells

Willis, David K.; Wang, Jun; Lindholm, Joliene R.; Orth, Anthony P.; Goodman, Walter G.

doi:10.1673/031.010.6601

Cited by 21 publications

(10 citation statements)

References 33 publications

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“…Not much is known about which genes are transcriptionally regulated by the JH activated receptor complex [161]. The gene kruppel-homolog 1 ( krh1 ) has been described as a JH response gene, inhibiting 20E induced broad ( br ) expression in D. melanogaster , but not in the specific context of oogenesis [159].…”

Section: Discussionmentioning

confidence: 99%

Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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BackgroundButterflies are popular model organisms to study physiological mechanisms underlying variability in oogenesis and egg provisioning in response to environmental conditions. Nothing is known, however, about; the developmental mechanisms governing butterfly oogenesis, how polarity in the oocyte is established, or which particular maternal effect genes regulate early embryogenesis. To gain insights into these developmental mechanisms and to identify the conserved and divergent aspects of butterfly oogenesis, we analysed a de novo ovarian transcriptome of the Speckled Wood butterfly Pararge aegeria (L.), and compared the results with known model organisms such as Drosophila melanogaster and Bombyx mori.ResultsA total of 17306 contigs were annotated, with 30% possibly novel or highly divergent sequences observed. Pararge aegeria females expressed 74.5% of the genes that are known to be essential for D. melanogaster oogenesis. We discuss the genes involved in all aspects of oogenesis, including vitellogenesis and choriogenesis, plus those implicated in hormonal control of oogenesis and transgenerational hormonal effects in great detail. Compared to other insects, a number of significant differences were observed in; the genes involved in stem cell maintenance and differentiation in the germarium, establishment of oocyte polarity, and in several aspects of maternal regulation of zygotic development.ConclusionsThis study provides valuable resources to investigate a number of divergent aspects of butterfly oogenesis requiring further research. In order to fully unscramble butterfly oogenesis, we also now also have the resources to investigate expression patterns of oogenesis genes under a range of environmental conditions, and to establish their function.

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Section: Discussionmentioning

confidence: 99%

Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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“…We conducted studies using a newly developed D. magna DNA microarray to investigate the transcriptome responses of daphnids to three JH agonists. The microarray technologies have been successfully used to identify genes responding to JH analogs in several insects, for which there are an abundant amount of sequence information (Willis et al, 2010;Zhu et al, 2010;Zou et al, 2013). This study revealed that the gene response provides insight into the biological and potentially toxicological responses to JH analogs, which are endocrine disrupting chemicals for daphnids.…”

Section: Discussionmentioning

confidence: 99%

Molecular impact of juvenile hormone agonists on neonatal Daphnia magna

Toyota

Kato

Miyakawa

et al. 2013

J of Applied Toxicology

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Daphnia magna has been used extensively to evaluate organism- and population-level responses to pollutants in acute toxicity and reproductive toxicity tests. We have previously reported that exposure to juvenile hormone (JH) agonists results in a reduction of reproductive function and production of male offspring in a cyclic parthenogenesis, D. magna. Recent advances in molecular techniques have provided tools to understand better the responses to pollutants in aquatic organisms, including D. magna. DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to JH agonists: methoprene (125, 250 and 500 ppb), fenoxycarb (0.5, 1 and 2 ppb) and epofenonane (50, 100 and 200 ppb). Exposure to these JH analogs resulted in chemical-specific patterns of gene expression. The heat map analyses based on hierarchical clustering revealed a similar pattern between treatments with a high dose of methoprene and with epofenonane. In contrast, treatment with low to middle doses of methoprene resulted in similar profiles to fenoxycarb treatments. Hemoglobin and JH epoxide hydrolase genes were clustered as JH-responsive genes. These data suggest that fenoxycarb has high activity as a JH agonist, methoprene shows high toxicity and epofenonane works through a different mechanism compared with other JH analogs, agreeing with data of previously reported toxicity tests. In conclusion, D. magna DNA microarray is useful for the classification of JH analogs and identification of JH-responsive genes.

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“…They have typically been used to study intraspecific changes in gene expression. However, producing microarrays is time and labor intensive, and microarrays representing large scale portions of genomes exist only for the most commonly researched model organisms such as humans, fruitfly, nematode and zebrafish (Lee et al 2004;Szameit et al 2008;Small et al 2009;Willis et al 2010). Additionally, there is a possibility of failing to detect relevant sample differences when conducting a cross species hybridization experiment.…”

Section: Cns Central Nervous Systemmentioning

confidence: 99%

Comparative genetics of the central nervous system in epigean and hypogean Astyanax mexicanus

Strickler

Soares

2011

Genetica

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The extreme environment of subterranean caves presents an adaptive challenge to troglobitic organisms. The mechanisms by which natural selection modify an ancestral surface neural circuit to produce a novel subterranean behavior remain a mystery. To address this question, we performed cross species microarray experiments to compare differences in gene expression levels in the adult brain of the teleost Astyanax mexicanus. This species provides a unique opportunity for comparative genetic studies as it consists of extant epigean (surface) and hypogean (cave) conspecifics. Microarray experiments herein revealed significant changes in transcription levels of seventeen genes, several of which are important for behaviors involved in metabolic management. We focused on genes central to three neurotransmission and neuromodulation networks: the endocannabinoid system (Cannabinoid receptor CB1), the dopaminergic system (Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein) and the glutamatergic system (glutamate receptor AMPA 2a). All three genes were upregulated in the hypogean form of A. mexicanus compared to the epigean form, indicating that behavioral differences in the hypogean form of the species could be due to alterations in expression levels of several key genes. This information provides insights into the complex relationships among environmental factors, genetics, nervous systems and adaptive behavior, and can subsequently help us understand how these interactions affect behavior in other biological systems.

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Microarray Analysis of Juvenile Hormone Response inDrosophila melanogasterS2 cells

Cited by 21 publications

References 33 publications

Unscrambling butterfly oogenesis

Unscrambling butterfly oogenesis

Molecular impact of juvenile hormone agonists on neonatal Daphnia magna

Comparative genetics of the central nervous system in epigean and hypogean Astyanax mexicanus

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