Microarray analysis of global gene regulation in the Cry1Ab‐resistant and Cry1Ab‐susceptible strains of <i>Diatraea saccharalis</i>

Guo, Zibiao; Zhu, Yu Cheng; Huang, Fangneng; Luttrell, R. G.; Leonard, Rogers

doi:10.1002/ps.2318

Cited by 32 publications

(30 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The expression of this gene was up-regulated after ingestion of bacteria-contaminated diet in the cabbage semilooper [38] and a similar gene was found to be involved in prophenoloxidase activation and nodulation response in the cotton bollworm [46]. Interestingly, a gene of the same class (Hdd11) was found induced in the Cry 1Ab resistant sugarcane borer [47] and in the beet armyworm after ingestion of B. thuringiensis VIP toxins [48], indicating its importance in defence against B. thuringiensis .…”

Section: Resultsmentioning

confidence: 99%

Oral immune priming with Bacillus thuringiensis induces a shift in the gene expression of Tribolium castaneum larvae

et al. 2017

View full text Add to dashboard Cite

BackgroundThe phenomenon of immune priming, i.e. enhanced protection following a secondary exposure to a pathogen, has now been demonstrated in a wide range of invertebrate species. Despite accumulating phenotypic evidence, knowledge of its mechanistic underpinnings is currently very limited. Here we used the system of the red flour beetle, Tribolium castaneum and the insect pathogen Bacillus thuringiensis (Bt) to further our molecular understanding of the oral immune priming phenomenon. We addressed how ingestion of bacterial cues (derived from spore supernatants) of an orally pathogenic and non-pathogenic Bt strain affects gene expression upon later challenge exposure, using a whole-transcriptome sequencing approach.ResultsWhereas gene expression of individuals primed with the orally non-pathogenic strain showed minor changes to controls, we found that priming with the pathogenic strain induced regulation of a large set of distinct genes, many of which are known immune candidates. Intriguingly, the immune repertoire activated upon priming and subsequent challenge qualitatively differed from the one mounted upon infection with Bt without previous priming. Moreover, a large subset of priming-specific genes showed an inverse regulation compared to their regulation upon challenge only.ConclusionsOur data demonstrate that gene expression upon infection is strongly affected by previous immune priming. We hypothesise that this shift in gene expression indicates activation of a more targeted and efficient response towards a previously encountered pathogen, in anticipation of potential secondary encounter.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3705-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Oral immune priming with Bacillus thuringiensis induces a shift in the gene expression of Tribolium castaneum larvae

et al. 2017

View full text Add to dashboard Cite

show abstract

“…For example, hdd11 was found to be highly induced in midguts of a Cry1Ab-resistant strain of Diatraea saccharalis [39], and Hdd23 was up-regulated in Lepidoptera infected by virus or bacteria [70,71]. Another gene of this family, Hdd1 , was also up-regulated in midguts of T. ni immune-induced by bacterial feeding [16].…”

Section: Resultsmentioning

confidence: 99%

“…This contrasts with studies performed in immune-induced lepidopterans (such as T. ni fed with bacteria [16]), or with coleopterans (such as T. molitor fed with Cry3Aa [34] or T. castaneum fed with Cry3Aa or Cry23Aa/Cry37Aa [37]), where apolipophorin III was up-regulated. Indeed, Cry1Ab-resistant D. saccharalis [39] showed constitutive up-regulation of an apolipophorin precursor. Typically, lipophorins I and II are considered insect hemolymph proteins involved in lipid transport, but lipophorin III has been also implied in defense mechanisms by clotting [21,61].…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Analysis of Gene Expression Profiles of the Beet Armyworm Spodoptera exigua Larvae Challenged with Bacillus thuringiensis Vip3Aa Toxin

et al. 2013

View full text Add to dashboard Cite

Host-pathogen interactions result in complex relationship, many aspects of which are not completely understood. Vip proteins, which are Bacillus thuringensis (Bt) insecticidal toxins produced during the vegetative stage, are selectively effective against specific insect pests. This new group of Bt proteins represents an interesting alternative to the classical Bt Cry toxins because current data suggests that they do not share the same mode of action. We have designed and developed a genome-wide microarray for the beet armyworm Spodoptera exigua, a serious lepidopteran pest of many agricultural crops, and used it to better understand how lepidopteran larvae respond to the treatment with the insecticidal protein Vip3Aa. With this approach, the goal of our study was to evaluate the changes in gene expression levels caused by treatment with sublethal doses of Vip3Aa (causing 99% growth inhibition) at 8 and 24 h after feeding. Results indicated that the toxin provoked a wide transcriptional response, with 19% of the microarray unigenes responding significantly to treatment. The number of up- and down-regulated unigenes was very similar. The number of genes whose expression was regulated at 8 h was similar to the number of genes whose expression was regulated after 24 h of treatment. The up-regulated sequences were enriched for genes involved in innate immune response and in pathogen response such as antimicrobial peptides (AMPs) and repat genes. The down-regulated sequences were mainly unigenes with homology to genes involved in metabolism. Genes related to the mode of action of Bt Cry proteins were found, in general, to be slightly overexpressed. The present study is the first genome-wide analysis of the response of lepidopteran insects to Vip3Aa intoxication. An insight into the molecular mechanisms and components related to Vip intoxication will allow designing of more effective management strategies for pest control.

show abstract

“…- Guo et al (2012) analysed gene regulation patterns in laboratory-selected Cry1Ab-resistant (Cry1Ab-R) and Cry1Ab-susceptible (Cry1Ab-S) sugarcane borer (SCB, Diatraea saccharalis) strains. Microarray analyses of 7145 cDNAs revealed 384 differentially expressed genes between larvae of the Cry1Ab-R and Cry1Ab-S strains.…”

Section: Environmental Risk Assessmentmentioning

confidence: 99%

“…Maize MON 810 had less leaf damage, and fewer surviving larvae were recovered from the whole plant, compared to non-Btmaize plants. The authors concluded that maize MON 810 may be used to control ASB and SSB, but that its efficacy also needs to be evaluated under field conditions.- Guo et al (2012) analysed gene regulation patterns in laboratory-selected Cry1Ab-resistant (Cry1Ab-R) and Cry1Ab-susceptible (Cry1Ab-S) sugarcane borer (SCB, Diatraea saccharalis) strains. Microarray analyses of 7145 cDNAs revealed 384 differentially expressed genes between larvae of the Cry1Ab-R and Cry1Ab-S strains.…”

mentioning

confidence: 99%

Scientific Opinion updating the risk assessment conclusions and risk management recommendations on the genetically modified insect resistant maize MON 810

2012

EFS2

View full text Add to dashboard Cite

Following a request from the European Commission, the Panel on Genetically Modified Organisms of the European Food Safety Authority (EFSA GMO Panel) compiled its previous risk assessment conclusions and risk management recommendations on the genetically modified insect resistant maize MON 810, and considered their validity in the light of new relevant scientific publications published from 2009 onwards. Following a search of the scientific literature published between 2009 and October 2012, the EFSA GMO Panel identified 165 peer-reviewed publications containing evidence specific to the risk assessment and/or management of maize MON 810, of which 68 publications were discussed and/or cited in previous EFSA GMO Panel scientific outputs. From the remaining 97 publications, eight were relevant for the molecular characterisation, 27 for food and feed safety assessment, 55 for the environmental risk assessment and/or risk management, two for the molecular characterisation and the environmental risk assessment and/or risk management and five for the food and feed safety assessment and the environmental risk assessment and/or risk management of maize MON 810. None of these publications reported new information that would invalidate the previous conclusions on the safety of maize MON 810 made by the EFSA GMO Panel. Therefore, the EFSA GMO Panel considers that its previous risk assessment conclusions on maize MON 810, as well as its previous recommendations on risk mitigation measures and monitoring, remain valid and applicable. DOI: https://doi.org/10. 2903/j.efsa.2012.3017 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-75246 Published Version Originally published at: Arpaia, Salvatore; Birch, Andrew N E; Chesson, Andrew; du Jardin, Patrick; Gathmann, Achim; Gropp, Jürgen; Herman, Lieve; Hoen-Sorteberg, Hilde-Gunn; Jones, Huw; Kiss, Jozsef; Kleter, Gijs; Lovik, Martinus; Messéan, Antoine; Naegeli, Hanspeter; Nielsen, Kaare Magne; Ovesna, Jaroslava; Perry, Joe; Rostoks, Nils; Tebbe, Christoph (2012) peer-reviewed publications containing evidence specific to the risk assessment and/or management of maize MON 810, of which 68 publications were discussed and/or cited in previous EFSA GMO Panel scientific outputs. From the remaining 97 publications, eight were relevant for the molecular characterisation, 27 for food and feed safety assessment, 55 for the environmental risk assessment and/or risk management, two for the molecular characterisation and the environmental risk assessment and/or risk management and five for the food and feed safety assessment and the environmental risk assessment and/or risk management of maize MON 810. None of these publications reported new information that would invalidate the previous conclusions on the safety of maize MON 810 made by the EFSA GMO Panel. Therefore, the EFSA GMO Panel considers that its previous risk assessment conclusions on maize MON 810, as well as its previous recommendations on risk mitigation measur...

show abstract

Microarray analysis of global gene regulation in the Cry1Ab‐resistant and Cry1Ab‐susceptible strains of Diatraea saccharalis

Cited by 32 publications

References 64 publications

Oral immune priming with Bacillus thuringiensis induces a shift in the gene expression of Tribolium castaneum larvae

Oral immune priming with Bacillus thuringiensis induces a shift in the gene expression of Tribolium castaneum larvae

Comprehensive Analysis of Gene Expression Profiles of the Beet Armyworm Spodoptera exigua Larvae Challenged with Bacillus thuringiensis Vip3Aa Toxin

Scientific Opinion updating the risk assessment conclusions and risk management recommendations on the genetically modified insect resistant maize MON 810

Contact Info

Product

Resources

About