Microarray analysis of gene expression in multiple sclerosis and EAE identifies 5-lipoxygenase as a component of inflammatory lesions

CYFIP2 is highly abundant in CD4+ cells from multiple sclerosis patients and is involved in T cell adhesion

“…Unless otherwise indicated, 10×10 6 Jurkat or CD4 + cells were used to determine active Rac-1 levels.…”

“…Although others have used this approach to determine changes in gene expression in MS lesions [5][6][7], there have been no reports about the changes in gene expression in specific immune cell types. Accordingly, we purified CD4 + and CD8 + cells from both MS patients that had disease activity within the past 6 months and were not receiving any therapeutic intervention and healthy age-matched controls and determined gene expression profiles.…”

Gene Expression Pattern Characterises Development of Multiple Sclerosis

“…The genes with increased expression in acute plaques included leukotriene A-4 hydroxylase, TNF α receptor, the auto-antigen annexin XI, interferon regulatory factor 2 (IRF-2), activin Type II receptor (ACVR2), protein kinase C type β-1 (PRKCB1), myelin transcription factor-1 (MYT1) and many several candidates. Two years later, Withney (Whitney et al, 2001) undertook microarray experiments using 2 MS patients. One patient's 16 chronic inactive (silent) plaques and the second patient's acute and chronic active plaque were used in the investigation.…”

Functional genomics and psychiatric illness

“…To date, microarray analysis has been used to investigate differential gene expression in several areas of brain research including aging (Ly et al, 2000;Lee et al, 2000;Weindruch et al, 2002), multiple sclerosis (Whitney et al, 1999(Whitney et al, , 2001) and Alzheimer's Disease (Ginsberg et al, 1999(Ginsberg et al, , 2000. However, the utility of microarray analysis for the investigation of molecular correlates of psychiatric illness is only now being realized.…”