Microarray Analysis of Equine Endometrium at Days 8 and 12 of Pregnancy1

Merkl, M.; Ulbrich, Susanne E.; Otzdorff, Christiane; Herbach, Nadja; Wanke, Rüdiger; Wolf, Eckhard; Handler, Johannes; Bauersachs, Stefan

doi:10.1095/biolreprod.110.085233

Cited by 85 publications

(96 citation statements)

References 106 publications

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“…The regulation of the uterine transcriptome during the preimplantation phase has been intensively studied in several species (sheep (73), bovine (74,75), horse (21,76), and pig (77)(78)(79)) and the proteome of uterine fluid during the early stages of pregnancy was analyzed in cattle and sheep (80 -82). Several proteins we found more abundant in the uterine luminal fluid during the luteal phase were also described as uterine markers of early pregnancy with an increased production in the presence of an embryo.…”

Section: Discussionmentioning

confidence: 99%

Proteomes of the Female Genital Tract During the Oestrous Cycle

Soleilhavoup

Riou

Tsikis

et al. 2016

Molecular & Cellular Proteomics

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The success of fertilisation in mammals is linked to the correct migration of spermatozoa in the different compartments of the female genital tract and the adequate timing of their interaction with the female gamete. In many mammalian species including human, the deposition of semen in the vagina is followed by the sperm migration through the cervix, the uterus and then the oviduct before reaching the site of fertilisation. This sperm transit within the female tract includes mechanical and biochemical interactions with the luminal fluids leading to selection of spermatozoa able to fertilize the oocyte.The first physiological barrier the spermatozoa will have to go through is the cervix whose vaginal side is covered by a highly viscous mucus, the cervical vaginal fluid (CVF) 1 . The CVF proteome was analyzed in humans in various physiological conditions (1, 2), mainly during pregnancy (3-7) or in several pathologies such as human papilloma virus (8) or candida albicans (9) infections. But few studies have investigated the CVF proteome during the cycle (10, 11). The amount of CVF increases at the time of ovulation concomitantly with a higher state of hydration and a reduced viscosity, to facilitate sperm migration (12). The mechanical properties of the mucus are essential to select spermatozoa with the highest fertilizing ability, i.e. with normal morphology and efficient mobility. The main structural components of the cervical mucus are mucins, highly glycosylated, high-molecular-weight proteins assembled into a filamentous and viscous mesh (13). The transcription of the mucin genes is increased at oestrus in the bovine cervical epithelium (14). The amount of mucins and their level of glycosylation are expected to contribute to the viscosity of the mucus (11). Therefore, the quantification of proteins such From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements,

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Section: Discussionmentioning

confidence: 99%

Proteomes of the Female Genital Tract During the Oestrous Cycle

Soleilhavoup

Riou

Tsikis

et al. 2016

Molecular & Cellular Proteomics

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“…In term human placental tissues, CRYAB was visibly observed in the stromal cells of the placental villi (Mineva et al 2008). In equine endometrium, CRYAB was detected on days 12 and 13.5 of pregnancy (Klein et al 2010, Merkl et al 2010. During the menstrual cycle, CRYAB was expressed in human and baboon endometrium and also upregulated during the window of implantation (Gruidl et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Differential expression and regulation of Cryab in mouse uterus during preimplantation period

Tian¹,

Wang²,

Li³

et al. 2013

Reproduction

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The aim of this study was to examine the expression and regulation of the crystallin, alpha B (Cryab) gene in mouse uterus during the peri-implantation period by in situ hybridization and real-time PCR. There was no detectable Cryab mRNA signal on days 1-4 of pregnancy. On day 5 of pregnancy when embryo implanted, a high level of Cryab mRNA signal was found in the subluminal stroma surrounding the implanting blastocyst. On days 6-8, Cryab mRNA was strongly expressed in the primary decidua. By real-time PCR, a high level of Cryab expression was detected on days 7 and 8 of pregnancy, although Cryab expression was seen from days 1 to 8. Under in vivo and in vitro artificial decidualization, Cryab expression was significantly elevated. Compared with the progesterone-primed delayed implantation uterus, a high level of Cryab mRNA expression was observed in estrogen-activated implantation uterus. In the uterine stromal cells, cAMP, estrogen, and progesterone could induce the expression of Cryab gene. In the ovariectomized mouse uterus, estrogen could also induce the expression of Cryab while progesterone inhibited its expression. Our data suggest that Cryab may play an important role during mouse embryo implantation and decidualization and that estrogen and progesterone can regulate the expression of Cryab gene.

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“…These include ischemic tolerance in the brain or heart [40,41], regulation of cellular metabolism by targeting mitochondria [42,43] and association with angiogenesis in endothelial cells [44]. Quite recently, the presence of endometrial STC1 in pregnant mares was reported [45]. Although receptors possibly located on the conceptus or functions of endometrial STC1 have not been established in the mare, STC1 could have a role in regulating embryonic calcium and/or phosphorus concentrations at a time in which the conceptus is not supported by a functional placenta.…”

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confidence: 99%

Production of Calcium Maintenance Factor Stanniocalcin-1 (STC1) by the Equine Endometrium During the Early Pregnant Period

et al. 2011

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Abstract.A factor responsible for progression to pregnancy establishment in the mare has not been definitively characterized. To identify factors possibly involved in the establishment of equine pregnancy, the endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19 and 25 pregnant animals. From initial subtractive hybridization studies, a calcium regulating factor, Stanniocalcin-1 (STC1) mRNA, was found as a candidate molecule expressed uniquely in the pregnant endometrium. Endometrial expression of STC1 mRNA was noted on day 19 and was markedly increased in the day 25 gravid endometrium. STC1 protein was found in the extracts of day 25 gravid endometrium and immunochemically localized in the uterine glands. In addition, STC1 protein was detected in uterine flushing media collected from day 25 pregnant mares. High concentrations of estradiol-17β (E2) were detected in day 25 conceptuses. E2 levels were much higher in the gravid endometrium than in other regions, whereas progesterone levels did not differ among the samples from different endometrial regions. Expression of STC1 mRNA, however, was not significantly upregulated in cultured endometrial explants treated with various concentrations of E2 (0.01-100 ng/ml) with or without 10 ng/ml progesterone. These results indicate that an increase in STC1 expression appears to coincide with capsule disappearance in the conceptus, and suggest that STC1 from the uterine glands likely plays a role in conceptus development during the pregnancy establishment period in the mare. Key words: Endometrium, Equine, Estradiol-17β, Stanniocalcin-1 (STC1), Uterine secretion (J. Reprod. Dev. 57: [203][204][205][206][207][208][209][210][211] 2011) he establishment of equine pregnancy is a unique and long process, during which a series of physical and possibly biochemical interactions are required between the conceptus and uterus. The equine conceptus first enters into the uterus on days 6-8 of pregnancy (day 0=day of ovulation) [1] and then begins to transmigrate throughout the entire uterine body and horns until approximately day 16 of pregnancy [2][3][4]. The frequency at which a conceptus moves from one uterine horn to another increases up to 10-20 times per day [5]. The conceptus ceases migration and lodges at the caudal portion of one of the uterine horns, known as "fixation", on approximately days 16-18 of pregnancy [2]. Between days 11 and 16 of pregnancy, the conceptus rapidly expands its volume, but keeps its spherical shape surrounded by a unique, smooth structure called a "capsule" [6,7] that emerges beneath the zona pellucida on days 6-8 of pregnancy. The capsule is an acellular mucin-like glycoprotein [8] that is structurally strong and is considered essential for the survival of the conceptus and continuation of pregnancy [9,10]. Together with peristaltic myometrial contractions, the capsule is likely to give motility to the conceptus, although the precise stimulus for conceptus migration is unknown. Thereafter, the capsule disappears by d...

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Microarray Analysis of Equine Endometrium at Days 8 and 12 of Pregnancy1

Cited by 85 publications

References 106 publications

Proteomes of the Female Genital Tract During the Oestrous Cycle

Proteomes of the Female Genital Tract During the Oestrous Cycle

Differential expression and regulation of Cryab in mouse uterus during preimplantation period

Production of Calcium Maintenance Factor Stanniocalcin-1 (STC1) by the Equine Endometrium During the Early Pregnant Period

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