Microarray Analysis and Detection of MicroRNAs Associated with Chronic Thromboembolic Pulmonary Hypertension

Abstract

The aim of this study was to understand the importance of chronic thromboembolic pulmonary hypertension- (CTEPH-) associated microRNAs (miRNAs). miRNAs differentially expressed in CTEPH samples compared with control samples were identified, and the target genes were predicted. The target genes of the key differentially expressed miRNAs were analyzed, and functional enrichment analyses were carried out. Finally, the miRNAs were detected using RT-PCR. Among the downregulated miRNAs, MiR-3148 regulated the most target genes and was significantly enriched in pathways in cancer, glioma, and ErbB signaling pathway. Furthermore, the number of target genes coregulated by miR-3148 and other miRNAs was the most. AR (androgen receptor), a target gene of hsa-miR-3148, was enriched in pathways in cancer. PRKCA (Protein Kinase C Alpha), also a target gene of hsa-miR-3148, was enriched in 15 of 16 KEGG pathways, such as pathways in cancer, glioma, and ErbB signaling pathway. In addition, the RT-PCR results showed that the expression of hsa-miR-3148 in CTEPH samples was significantly lower than that in control samples (P < 0.01). MiR-3148 may play an important role in the development of CTEPH. The key mechanisms for this miRNA may be hsa-miR-3148-AR-pathways in cancer or hsa-miR-3148-PRKCA-pathways in cancer/glioma/ErbB signaling pathway.

A novel cyclic biased agonist of the apelin receptor, MM07, is disease modifying in the rat monocrotaline model of pulmonary arterial hypertension

“…MCT-induced PAH(Falcão-Pires et al, 2009). The apelin signalling pathway is also implicated in pulmonary veno-occlusive disease(Lathen et al, 2014) and chronic thromboembolic pulmonary hypertension(Miao et al, 2017). As expected, agents that activate apelin expression or act as downstream effectors of apelin signalling have demonstrated beneficial effects in PAH animal models(Bertero et al, 2014;Kim et al, 2013;Nickel et al, 2015;Spiekerkoetter et al, 2013).…”

A preliminary study of the effect of a high-salt diet on transcriptome dynamics in rat hypothalamic forebrain and brainstem cardiovascular control centers

“…The downregulation of Snrpd2l, small nuclear ribonucleoprotein D2-like suggests that aspects of RNA processing are changed in NTS due to HS diet. Meanwhile, the Csrnp3 has been reported as one of the targeted genes in causing HPN (Miao et al, 2017;Yamamoto et al, 2013); however, the functional role of it was not discussed. The Rnf157, a ring finger protein 157, has been described to predominantly be expressed in brain and is implicated in the regulation of neuronal survival (Matz et al, 2015).…”

MicroRNA-3148 acts as molecular switch promoting malignant transformation and adipocytic differentiation of immortalized human bone marrow stromal cells via direct targeting of the SMAD2/TGFβ pathway

“…Elevated levels of miR-3148 were also associated with congestive heart failure (CHF) in a cohort of 44 CHF patients and 15 healthy subjects 41 . In addition, downregulation of miR-3148 was observed in chronic pulmonary hypertension 42 .…”