Microanalytical subcellular fractionation of rat liver homogenates by poly(ethylene glycol)/dextran countercurrent partition

Morris, Wesley B.

doi:10.1042/bst0080076

Cited by 5 publications

(2 citation statements)

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“…ENTs play essential roles in the control of adenosine homeostasis. The function of ENT1 in the transport of adenosine, regulating cellular functions in the brain, has been extensively studied [ 214 ]. As described above, several BBB-permeable ENT1 inhibitors have recently been developed as potential drug candidates for the treatment of multiple neurodegenerative diseases, including AD, HD, ALS, and epilepsy [ 46 , 111 , 127 , 141 , 144 ].…”

Section: Introductionmentioning

confidence: 99%

Emerging roles of dysregulated adenosine homeostasis in brain disorders with a specific focus on neurodegenerative diseases

Chang

Lin

et al. 2021

J Biomed Sci

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In modern societies, with an increase in the older population, age-related neurodegenerative diseases have progressively become greater socioeconomic burdens. To date, despite the tremendous effort devoted to understanding neurodegenerative diseases in recent decades, treatment to delay disease progression is largely ineffective and is in urgent demand. The development of new strategies targeting these pathological features is a timely topic. It is important to note that most degenerative diseases are associated with the accumulation of specific misfolded proteins, which is facilitated by several common features of neurodegenerative diseases (including poor energy homeostasis and mitochondrial dysfunction). Adenosine is a purine nucleoside and neuromodulator in the brain. It is also an essential component of energy production pathways, cellular metabolism, and gene regulation in brain cells. The levels of intracellular and extracellular adenosine are thus tightly controlled by a handful of proteins (including adenosine metabolic enzymes and transporters) to maintain proper adenosine homeostasis. Notably, disruption of adenosine homeostasis in the brain under various pathophysiological conditions has been documented. In the past two decades, adenosine receptors (particularly A1 and A2A adenosine receptors) have been actively investigated as important drug targets in major degenerative diseases. Unfortunately, except for an A2A antagonist (istradefylline) administered as an adjuvant treatment with levodopa for Parkinson’s disease, no effective drug based on adenosine receptors has been developed for neurodegenerative diseases. In this review, we summarize the emerging findings on proteins involved in the control of adenosine homeostasis in the brain and discuss the challenges and future prospects for the development of new therapeutic treatments for neurodegenerative diseases and their associated disorders based on the understanding of adenosine homeostasis.

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Section: Introductionmentioning

confidence: 99%

Emerging roles of dysregulated adenosine homeostasis in brain disorders with a specific focus on neurodegenerative diseases

Chang

Lin

et al. 2021

J Biomed Sci

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“…The application of phase partition to the resolution of fragile organelles such as lysosomes and peroxisomes has also been explored by Morris & Peters (1980) using a small-volume counter-current distribution apparatus described by Pritchard et al (1975). Drug-cell interactions.…”

Section: Properties Ofphase Systemsmentioning

confidence: 99%

The separation of cells and organelles by partitioning in two-polymer aqueous phases

Fisher¹

1981

Biochemical Journal

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Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System

Bandyopadhyay

1987

Cell Separation

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Microanalytical subcellular fractionation of rat liver homogenates by poly(ethylene glycol)/dextran countercurrent partition

Cited by 5 publications

References 0 publications

Emerging roles of dysregulated adenosine homeostasis in brain disorders with a specific focus on neurodegenerative diseases

Emerging roles of dysregulated adenosine homeostasis in brain disorders with a specific focus on neurodegenerative diseases

The separation of cells and organelles by partitioning in two-polymer aqueous phases

Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System

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