Microalgal extract from thermotolerant <i>Coelastrella</i> sp. <scp>F50</scp> retards the liver tumor progression by targeting hepatic cancer stem cells

Chang, Yu‐Chan; Chu, Tian‐Huei; Yu, Po-Chien; Wang, E‐Ming; Huang, Chao‐Cheng; Hu, Tsung–Hui; Wen, Zhi‐Hong; Ko, C. M.; Chen, Ching-Nen Nathan; Tai, Ming‐Hong

doi:10.1002/ptr.7111

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…The assay was performed as previously described ( 57 ). Liver cancer cells were suspended in serum-free Dulbecco's modified Eagle's medium/F12 (Gibco) medium containing B-27 (Gibco), 20 ng/ml EGF (PeproTech), and 20 ng/ml bFGF (PeproTech).…”

Section: Methodsmentioning

confidence: 99%

“…The assay was performed as previously described (57). 5 × 10 5 HCC cells were plated at 6-well, and then cells were treated with rLECT2 in serum-free medium for 24 h. After cell harvesting, HCC cells were suspended in Hanks' Balanced Saline Solution (HBSS) (Gibco) containing with 5% fetal bovine serum (Hyclone) and 5 μg/mL Hoechest 33342 (Sigma-Aldrich) for 90 minutes at 37℃.…”

Section: Side Population Cells Assaymentioning

confidence: 99%

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Leukocyte cell-derived chemotaxin 2 regulates epithelial-mesenchymal transition and cancer stemness in hepatocellular carcinoma

Chu¹,

Ko²,

Tai³

et al. 2022

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Section: Side Population Cells Assaymentioning

confidence: 99%

Leukocyte cell-derived chemotaxin 2 regulates epithelial-mesenchymal transition and cancer stemness in hepatocellular carcinoma

Chu¹,

Ko²,

Tai³

et al. 2022

Journal of Biological Chemistry

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“…Evaluation of the effects of the microalgal extract and pure pigment on endothelial cell function could be related to their ability to prevent angiogenic switch, hence reducing tumor progression. Several studies have investigated the effects of microalgae, including their extracts and pigments, on cancer [ 14 , 25 , 26 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ], but much less has been reported about marine pigments in cancer-related angiogenesis [ 69 , 70 , 71 , 72 ].…”

Section: Introductionmentioning

confidence: 99%

Skeletonema marinoi Extracts and Associated Carotenoid Fucoxanthin Downregulate Pro-Angiogenic Mediators on Prostate Cancer and Endothelial Cells

Calabrone

Carlini

Noonan

et al. 2023

Cells

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The exploration of natural preventive molecules for nutraceutical and pharmaceutical use has recently increased. In this scenario, marine microorganisms represent an underestimated source of bioactive products endowed with beneficial effects on health that include anti-oxidant, anti-inflammatory, differentiating, anti-tumor, and anti-angiogenic activities. Here, we tested the potential chemopreventive and anti-angiogenic activities of an extract from the marine coastal diatom Skeletonema marinoi Sarno and Zingone (Sm) on prostate cancer (PCa) and endothelial cells. We also tested one of the main carotenoids of the diatom, the xanthophyll pigment fucoxanthin (Fuco). Fuco from the literature is a potential candidate compound involved in chemopreventive activities. Sm extract and Fuco were able to inhibit PCa cell growth and hinder vascular network formation of endothelial cells. The reduced number of cells was partially due to growth inhibition and apoptosis. We studied the molecular targets by qPCR and membrane antibody arrays. Angiogenesis and inflammation molecules were modulated. In particular, Fuco downregulated the expression of Angiopoietin 2, CXCL5, TGFβ, IL6, STAT3, MMP1, TIMP1 and TIMP2 in both prostate and endothelial cells. Our study confirmed microalgae-derived drugs as potentially relevant sources of novel nutraceuticals, providing candidates for potential dietary or dietary supplement intervention in cancer prevention approaches.

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“…In addition, the extract from Coelastrella sp. F50 has been demonstrated to display antitumor activity in hepatocellular carcinoma [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Formation of Lutein, β-Carotene and Astaxanthin in a Coelastrella sp. Isolate

Ali

Vorisek

Dowd³

et al. 2022

Molecules

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In this study, the effect of media composition, N/P ratio and cultivation strategy on the formation of carotenoids in a Coelastrella sp. isolate was investigated. A two-stage process utilizing different media in the vegetative stage, with subsequent re-suspension in medium without nitrate, was employed to enhance the formation of carotenoids. The optimal growth and carotenoid content (β-carotene and lutein) in the vegetative phase were obtained by cultivation in M-8 and BG11 media. Use of a N/P ratio of 37.5 and low light intensity of 40 μmol m−2 s−1 (control conditions) led to optimal biomass production of up to 1.31 g L−1. Low concentrations of astaxanthin (maximum of 0.31 wt. %) were accumulated under stress conditions (nitrogen-deficient medium containing 1.5 % of NaCl and light intensity of 500 μmol m−2 s−1), while β-carotene and lutein (combined maximum of 2.12 wt. %) were produced under non-stress conditions. Lipid analysis revealed that palmitic (C16:0) and oleic (C18:1) constituted the main algal fatty acid chains (50.2 ± 2.1% of the total fatty acids), while esterifiable lipids constituted 17.2 ± 0.5% of the biomass by weight. These results suggest that Coelastrella sp. could also be a promising feedstock for biodiesel production.

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Microalgal extract from thermotolerant Coelastrella sp. F50 retards the liver tumor progression by targeting hepatic cancer stem cells

Cited by 8 publications

References 37 publications

Leukocyte cell-derived chemotaxin 2 regulates epithelial-mesenchymal transition and cancer stemness in hepatocellular carcinoma

Leukocyte cell-derived chemotaxin 2 regulates epithelial-mesenchymal transition and cancer stemness in hepatocellular carcinoma

Skeletonema marinoi Extracts and Associated Carotenoid Fucoxanthin Downregulate Pro-Angiogenic Mediators on Prostate Cancer and Endothelial Cells

Formation of Lutein, β-Carotene and Astaxanthin in a Coelastrella sp. Isolate

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