Microalgal cell disruption in a high-power ultrasonic flow system

Wang, Meng; Yuan, Wenqiao

doi:10.1016/j.biortech.2015.06.040

Cited by 33 publications

(33 citation statements)

References 31 publications

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“…Instead, mechanical methods require only devices that generate shear force, impulsive force, pressure, and/or cavitation impulsive force, all of which can be variously achieved using a high‐pressure homogenizer, a bead mill, an ultrasound, a microwave, or a high‐speed homogenizer . Many studies have been conducted on the mechanical cell disruption of Chlorella vulgaris , Nannochloropsis oculata , and Nannochloropsis sp., which store lipids inside their cells . However, to the best of our knowledge, only two studies () have been examined for the mechanical disruption of B. braunii .…”

Section: Introductionmentioning

confidence: 99%

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Tsutsumi

Yokomizo

Saito

et al. 2017

Asia-Pacific J Chem Eng

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The colonial microalga, Botryococcus braunii, produces and stores hydrocarbons in membranes and in colonies. Although hydrocarbons in B. braunii could easily be extracted for biofuel production, yields are actually poor without energy-intensive pretreatment such as thermal drying. To develop extraction methods without drying, we applied the mechanical cell disruption to wet B. braunii using either a high-pressure homogenizer, a bead mill, or a circulating particle disruptor (Jet-Paster) and examined the relationships between the extent of cell and/or colony disruption and extracted hydrocarbon yields using n-hexane. When the number of particles over 20 μm classified as colonies, decreased after each treatment, the hydrocarbon yields of samples treated (over 20%) were much larger than those without treatments (under 5%). Thus, the colony disruption may increase hydrocarbon yields by enhancing n-hexane penetration to the colony. Although the degree of disruption on the Jet-Paster treatment was the lowest (2.2-9.3%) and that of sample treated by the homogenizer was the highest (27-55%), extracted hydrocarbon yields were improved in both treatments. The facts indicate that disrupting large colonies into small colonies improves hydrocarbon recovery, and the fractionation of cells is not needed for hydrocarbon extraction from B. braunii. Figure 4. Distributions of roundness of before and after cell disruption ((a-1), (b-1), and (c-1)) Untreated samples. (a-2) High-pressure homogenizer (30 MPa). (a-3) High-pressure homogenizer (80 MPa). (b-2) Bead mill (8 m/s). (b-3) Bead mill (14 m/s). (c-2) Jet-Paster (4800 rpm). (c-3) Jet-Paster (7200 rpm). S. TSUTSUMI ET AL.Asia-Pacific Journal of Chemical Engineering 460

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Section: Introductionmentioning

confidence: 99%

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Tsutsumi

Yokomizo

Saito

et al. 2017

Asia-Pacific J Chem Eng

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“…This finding was similar to that of Cameron, who revealed that the protein, fat, and lactose content in milk was not influenced significantly by a high‐power ultrasound. The destruction of lipid was also not detected using Nile Red‐stained lipid fluorescence density . Therefore, the H 2 O 2 ‐aided pretreatment could be an effective pretreatment for the downstream comprehensive utilization of algal biomass.…”

Section: Resultsmentioning

confidence: 99%

“…As one of the methods, ultrasonication (US) draws increasing attention because of its advantages without an addition or separating process compared with a chemical technique or bead milling, respectively . In the course of sonication, generally, the rapid compression/decompression cycles of the acoustic waves generate numerous small cavitation bubbles in the algal suspension, and these bubbles then implode and impart forceful energy (mainly mechanical forces, such as liquid shear stress and localized high pressure) to the surrounded cell, consequently, resulting in disruption .…”

Section: Introductionmentioning

confidence: 99%

Evaluation on H₂O₂‐aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa

Duan

Tan

Dai

et al. 2017

Asia-Pacific J Chem Eng

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In this work, Chlorella pyrenoidosa was chosen as model microalgae, and several pretreatments, including hydroxyl-peroxide-aided ultrasonic pretreatments and mono-ultrasonication (US), were used to rupture the microalgal cells for extracting cellular inclusion. The disruption efficiency and the energy consumption of each technique were estimated through measuring the amount of released protein from cells and the microscopic images after pretreatment. Results showed that the combined pretreatments obtained a much higher lysis efficiency than did the US (over twofold). However, the concentration of H 2 O 2 used (ranging from 0.1 to 5 mM) failed to influence the lysis efficiency significantly (P < 0.05). Additionally, the acoustic energy efficiency was increased with the initial cell concentration treated, but the coefficient of utilization of algal biomass was decreased significantly. Therefore, an ideal initial cell concentration (13.4 × 10 7 cells/mL) was gained in terms of the higher coefficient of utilization of algal biomass. This research suggested that a synergistic technique could be an effective pretreatment for microalgal cell disruption and downstream comprehensive utilization.

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“…All purification steps were carried out at 4 °C. The cell pellet was resuspended in deionized water and broken by ultrasonic processor for an hour [22]. The supernatant after centrifugation (10,000 × g, 10 min) was used as the crude enzyme.…”

Section: Purification Of (R)-1-phenylethanol Dehydrogenasementioning

confidence: 99%

A System of Photocatalysis for NAD+ Regeneration of Product of (S)-1-Pheylethanol by Enzymic Catalysis

Zhang

Huang

et al. 2019

Bull. Chem. React. Eng. Catal.

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In this study, a system of photocatalysis for NAD + regeneration of enzymatic catalysis was constructed. The optimal conditions for the coupling reaction of photocatalysis and biocatalysis were explored. Blue light was chosen for the efficient reaction and the optimal concentration of VB2 (vitamin B2, riboflavin) was determined. NAD + -dependent (R)-1-phenylethanol dehydrogenase was used in the reaction for transforming (R)-1-phenylethanol to acetophenone. The byproducts of the reaction were just H2O and O2 by means of catalase. The coupling reaction of catalysis and photocatalysis can be used for obtaining (S)-1-phenylethanol through racemization of 1-phenylethanol.

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Microalgal cell disruption in a high-power ultrasonic flow system

Cited by 33 publications

References 31 publications

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Evaluation on H₂O₂‐aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa

A System of Photocatalysis for NAD+ Regeneration of Product of (S)-1-Pheylethanol by Enzymic Catalysis

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Microalgal cell disruption in a high-power ultrasonic flow system

Cited by 33 publications

References 31 publications

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Evaluation on H2O2‐aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa

A System of Photocatalysis for NAD+ Regeneration of Product of (S)-1-Pheylethanol by Enzymic Catalysis

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Evaluation on H₂O₂‐aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa