Microalgal biofuel production: Potential challenges and prospective research

Sathya, Arunachalam Bose; Thirunavukkarasu, Arunachalam; Nithya, Rajarathinam; Nandan, Abhishek; Sakthishobana, Krishnamoorthy; Kola, Anand Kishore; Sivashankar, R.; Tuấn, Hoàng Anh

doi:10.1016/j.fuel.2022.126199

Cited by 62 publications

(15 citation statements)

References 121 publications

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“…The photosynthetic capability of these microorganisms gives them extraordinary potential as possible biological tools to capture industrial CO 2 . They are also considered as energy crops for biofuels because of their high lipid content [ 5 , 24 , 25 ]. These microorganisms are also equally capable of producing a wide range of commercially interesting products such as fats, sugars, carotenoids, and many others [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Neutral Lipids of the Oleaginous Alga Micractinum inermum

Banskota,

Hui,

Jones

et al. 2024

Molecules

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An oleaginous microalga Micractinum inermum isolated from Mariana Lake, AB, Canada was cultured in a 1000 L photobioreactor with an f/2 medium to study its lipid content and neutral lipid profile. Algal biomass was collected at the stationary phase contained a significant amount of lipids (44.2%), as determined by Folch’s method. The lipid was fractionated into neutral lipid, glycolipid and phospholipid fractions. The neutral lipid constitutes almost 77.3% of the total lipid species and is mainly composed of triacylglycerols (TAGs) determined by a proton NMR study. UHPLC-HRMS analysis allows us for the first time to identify 81 TAGs in the neutral lipid fraction of M. inermum. The fatty acid acyl side chains were identified based on fragment ions observed in MSMS analysis. TAGs with fatty acid acyl chains 18:1/18:1/18:1, 18:1/18:1/16:0, 18:2/18:1/16:0, and 18:2/18:2/18:0 were the major ones among the identified TAGs. Fatty acid analysis further supports the fact that oleic acid was the major fatty acid present in the neutral lipid fraction of M. inermum constituting 41.7%, followed by linoleic acid at 21.5%, and palmitic acid at 21.2%. The saturated and monounsaturated fatty acids were 67.8% or higher in the lipid fraction. Long-chain fatty acids were only present in a minor quantity. The results clearly demonstrate that M. inermum is an excellent source for TAGs.

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Section: Discussionmentioning

confidence: 99%

Characterization of Neutral Lipids of the Oleaginous Alga Micractinum inermum

Banskota,

Hui,

Jones

et al. 2024

Molecules

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“…The processes that go into the production of bioethanol can be broken down into four categories: preparation of the feedstock, pretreatment with microbial enzymes (both bacterial and fungal), hydrolysis or saccharification, fermentation with a variety of microbes, including bacteria, fungi, and yeast, distillation, and dehydration as the final steps in the production process. In addition to the production of bioethanol, feedstock sources may also be utilized in the generation of lactic acid and various bio-based products [ 95 ]. Increasing the amount of bioethanol that can be made from different substrates requires careful consideration of the pretreatment methods that will be used.…”

Section: Types Of Biofuelsmentioning

confidence: 99%

“…Providing nutrients (e.g. nitrate and potassium) and fresh water for the microalgae industry is an expensive endeavor, and it is one of the main reasons why the price of producing advanced biofuels has increased when compared to the price of fossil fuel today, the cost of creating biodiesel from microalgae is significantly greater than that of conventional fuel sources [ 95 ]. Studies indicate that the cost of manufacturing biodiesel from microalgae can be between 20 and 30%, accounted for by these factors [ 140 ].…”

Section: Challenges and Prospectsmentioning

confidence: 99%

Bioengineering strategies of microalgae biomass for biofuel production: recent advancement and insight

Sundaram,

Rajendran,

Gnanasekaran

et al. 2023

Bioengineered

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Algae-based biofuel developed over the past decade has become a viable substitute for petroleum-based energy sources. Due to their high lipid accumulation rates and low carbon dioxide emissions, microalgal species are considered highly valuable feedstock for biofuel generation. This review article presented the importance of biofuel and the flaws that need to be overcome to ensure algae-based biofuels are effective for future-ready bioenergy sources. Besides, several issues related to the optimization and engineering strategies to be implemented for microalgae-based biofuel derivatives and their production were evaluated. In addition, the fundamental studies on the microalgae technology, experimental cultivation, and engineering processes involved in the development are all measures that are commendably used in the pre-treatment processes. The review article also provides a comprehensive overview of the latest findings about various algae species cultivation and biomass production. It concludes with the most recent data on environmental consequences, their relevance to global efforts to create microalgae-based biomass as effective biofuels, and the most significant threats and future possibilities.

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“…[ 12 ] Despite the fact that several strains of microalgae were employed in biodiesel production, only limited numbers of strains were able to show high potential in the accumulation of lipid in terms of its quality and quantity. [ 9 ] Screening native algae for species with desirable traits gives a robust biological platform for bioresource production given their pre‐adaptation to the prevailing regional abiotic and biotic factors. [ 13 ] Bioprospecting and utilizing of indigenous strains is benefited by local microalgae species isolating and selecting, regarding their physiological characteristics, as they are highly adapted to the local climatic environment, unused domestic biological resources utilization, and foreign species biological contamination prevention, when cultivated for large‐scale operations.…”

Section: Introductionmentioning

confidence: 99%

“…[ 8 ] Microalgae third‐generation biofuels have the most potential to substitute petroleum‐derived fuels without the drawbacks of first and second‐generation biofuels. [ 9 ] One of the great biological challenges associated with algal biofuels is the identification and bringing into culture of species with a favorable combination of the following characteristics: high growth rate, high lipid content and ease of harvest and extraction. [ 10 ] The selection of the microalgae strain is crucial for producing biofuel and high‐value co‐products to maximize revenue.…”

Section: Introductionmentioning

confidence: 99%

Indigenous microalgae strains characterization for a sustainable biodiesel production

Amouri,

Aziza,

Kaidi

et al. 2023

Biotechnology Journal

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Microalgae have been widely recognized as a promising feedstock for sustainable biofuels production to tackle global warming and pollution issues related to fossil fuels uses. This study identified and analyzed indigenous microalgae strains for biodiesel production, specifically Chlorella vulgaris and Coelastrella thermophila var. globulina, from two distinct locations in Algeria. Molecular identification confirmed their identity, and the microalgae exhibited notable growth characteristics. Local Chlorella vulgaris and Coelastrella thermophila var. globulina showed good growth and high biomass yield, compared to Chlorella vulgaris CCAP211/11B reaching a weight of 1.48 g L‐1, 1.95 g L‐1, and 2.10 g L‐1, respectively. Lipids content of local Chlorella vulgaris, Coelastrella thermophila var. globulina, and Chlorella vulgaris CCAP211/11B, were found to be 31.39 ± 3.3%, 17 ± 2.26%, and 19 ± 0.64%, respectively. Chlorella vulgaris stood out as a candidate for biodiesel production due to its equilibrium between SFA and PUFA (43.24% and 45.27%). FAs are predominated by SFA and MUFA for Coelastrella thermophila var. globulina with value of 81.49% (SFA+MUFA). Predicted biodiesel qualities comply with ASTM6751 and EN14214 standards.Studied microalgae have therefore a promising potential for biodiesel production. However, optimising cultivation conditions is necessary to enhance biomass and lipids yield at a large scale.

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Microalgal biofuel production: Potential challenges and prospective research

Cited by 62 publications

References 121 publications

Characterization of Neutral Lipids of the Oleaginous Alga Micractinum inermum

Characterization of Neutral Lipids of the Oleaginous Alga Micractinum inermum

Bioengineering strategies of microalgae biomass for biofuel production: recent advancement and insight

Indigenous microalgae strains characterization for a sustainable biodiesel production

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