Microalgae of different phyla display antioxidant, metal chelating and acetylcholinesterase inhibitory activities

Custódio, Luísa; Justo, Tiago; Silvestre, Leilane Gorga Gaspar Ruas; Barradas, Ana Maria; Vizetto‐Duarte, Catarina; Pereira, Hugo; Barreira, Luísa; Rauter, AP; Alberício, Fernando; Varela, João

doi:10.1055/s-0032-1320267

Cited by 21 publications

(32 citation statements)

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“…TPC has been reported recently for various microalgae species in relation to their antioxidant potential (Duval et al 2000;Li et al 2007;Goh et al 2010;Hajimahmoodi et al 2010;Custódio et al 2012;Goiris et al 2012). However, since plant-based phenolic compounds also play both beneficial and detrimental roles in animal nutrition (in particular protein bioavailability), their measurement is important and has not yet been reported for most of the species studied here.…”

Section: −1mentioning

confidence: 83%

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

2014

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=b8564d17-860a-4eb5-80c6-fd741c419b07 http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=b8564d17-860a-4eb5-80c6-fd741c419b07 Abstract Proximate, amino acid and elemental composition, total phenolic content (TPC), and in vitro protein digestibility (IVPD) of microalgal biomass were determined. Microalgae contained low to moderate ash (5-17 %), moderate to high carbohydrate (18-46 %), crude protein (18-46 %), high crude lipid (12-48 %), and energy (19-27 MJ kg −1 ). Characteristic of microalgae, non-essential amino acids (AAs), aspartic and glutamic acids, were predominant (20-30 % of protein; 8-12 % of dry weight). Microalgae had favorable essential AA profiles with high essential amino acid (EAA) indices (0.9-1.2). Expressed as gE A A1 0 0gp r o t e i n −1 , Porphyridium aerugineum was rich in leucine (11.9), lysine (8.0), arginine (8.6), and tryptophan (3.3); Nannochloropsis granulata (A) in leucine (11.0), lysine (8.5), and tryptophan (2.8); Tetraselmis chuii and Botryococcus braunii in arginine (9.4 and 20.5, respectively); and Phaeodactylum tricornutum in lysine (6.4) and tryptophan (2.6). Mineral compositions (%) were calcium (0.1-3.0), magnesium (0.3-0.7), phosphorous (0.7-1.5), potassium (0.7-2.4), sodium (0.8-2.7), and sulfur (0.4-1.4), and trace element compositions (mg kg −1 )we reco pp er(1 8 -102), iron (1,395-11,101), manganese (45-454), selenium (0-0.5), and zinc (28-64). Microalgae contained low TPC (6-13 mg gallic acid equivalents (GAE) g −1 DW), except T. chuii (20 mg GAE g −1 DW). IVPD was high (>90 %) for B. braunii (A), P. aerugineum, and lipid-extracted N. granulata (B); mid-range (80-89 %) for P. tricornutum, N. granulata (A), B. braunii (B), Neochloris oleoabundans, T. chuii, and whole N. granulata (B); and lower (<80 %) for Acutodesmus dimorphus.M i c r o a l g a ls p e c i e sP. tricornutum, B. braunii, N. granulata,a n dT. chuii had high protein (40-52 %), IVPD (82-97 %), and digestible protein (35-50 %), comparable to plant proteins used in animal feeds and aquaculture.

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Section: −1mentioning

confidence: 83%

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

2014

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“…In this field, research has made the greatest therapeutic advances in the treatment of AD by inhibiting AChE (Sramek et al ., ; Singh, ), and therefore, the search for other inhibitors of this enzyme has increased markedly. Some AChE inhibitor compounds have been found from various natural sources, mainly from plants (Murray et al ., ) as well as from animal proteins (Ahn et al ., ) or mushrooms (Patocka, ) or from marine photosynthetic organisms (Custódio et al., ).…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase‐inhibitor hydrolysates obtained from ‘in vitro’ enzymatic hydrolysis of mannoproteins extracted from different strains of yeasts

Spontón¹,

Spinelli²,

Drago³

et al. 2015

Int J of Food Sci Tech

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Summary In vitro inhibitory activity against acetylcholinesterase (AChE) of peptides obtained by enzymatic hydrolysis of mannoproteins extracted from strains of yeasts was investigated. Yeast mannoproteins were extracted from strains belonging to the genera Brettanomyces, Candida, Pichia and Saccharomyces isolated from dairy products. They were obtained by heat treatment in citrate buffer and purified by affinity chromatography with concanavalin A. Each purified extract was subsequently hydrolysed with proteolytic enzymes (trypsin, pepsin, chymotrypsin and proteinase K) applied individually or in combination, thus generating smaller peptides. Inhibitory activity of the latter against AChE was determined. The molecular weight of mannoproteins, determined by SDS‐PAGE, was between 6.5 and 30 kDa. As regards AChE inhibition, a preliminary screening of all hydrolysed extracts was performed, yielding variable results with 59% maximum inhibition. Subsequently, when inhibitory concentration 50 (IC50) was determined, the extracts showed higher inhibitory activity (between 6.75 and 12.3 mg mL−1). Results showed that the mannoproteins separated from yeast strains of food origin generated bioactive peptides by enzymatic hydrolysis, which can be of interest to the manufacturing of food with potential functional properties.

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“…Although many microalgal species have been reported widely for their antioxidant activity (Li et al, 2007;Rao et al, 2006;Duan et al, 2006;Wu et al, 2005;Herrero et al, 2006Herrero et al, , 2005Murthy et al, 2005;Tannin-Spitz et al, 2005;Kuda et al, 2005;Guzman et al, 2001;Mirada et al, 1998) there has been limited information on antioxidant levels of microalgae under varying culture conditions. Few studies reported that phenolic compounds had a high antioxidant capacity (Jaime et al, 2005;Geetha et al, 2010;Custodio et al, 2012), while another study found the opposite (Goh et al, 2010) hence it is not clear whether phenolic substances are important antioxidants in microalgae.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Total Phenolics Content and Antioxidant Activities of Microalgae Under Autotrophic, Heterotrophic and Mixotrophic Growth

Shetty¹,

Sibi²

2014

J. of Food Resource Science

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A study was carried out to explore the relationship between growth conditions and antioxidant properties of microalgae. Further, correlation between phenolics and antioxidant activities were studied to determine whether antioxidant activity depends on microalgal phenolics content under varying culture conditions. Total phenolics and antioxidant properties of Chlorella vulgaris and Scenedesmus obliquus grown under autotrophic, heterotrophic and mixotrophic conditions were evaluated. Domestic water, Bold's medium and sewage water were used to cultivate the microalgae and the extracts were prepared in methanol and analyzed for biochemical (total phenolics) and antioxidant properties (DPPH assay, super oxide scavenging assay and antioxidant potential). The experiments were done in triplicates and significant correlation coefficients between antioxidant properties against phenolic content and growth conditions were interrelated. The amount of total phenolics content varied in growth conditions and ranged from 0.11-0.55 mg GAE gG 1 . Significant correlation co-efficient between phenolics and antioxidant properties of microalgae determined by DPPH, superoxide anion scavenging and total antioxidant activities were found in the study. The strongest positive correlation was found to be between total phenolics and DPPH activity in C. vulgaris (r = 0.997). In S. obliquus, the strongest positive correlation was between total phenolics and antioxidant potential (r = 0.091) at p<0.01 followed by superoxide scavenging (p<0.05). The findings indicated that phenolic compounds were the major contributors to the antioxidant properties of microalgae. The results demonstrated that strongest positive correlation was observed in mixotrophic conditions followed by autotrophic conditions in Chlorella whereas the correlation was significant under heterotrophic conditions in Scenedesmus followed by mixotrophic conditions.

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Microalgae of different phyla display antioxidant, metal chelating and acetylcholinesterase inhibitory activities

Cited by 21 publications

References 0 publications

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

Acetylcholinesterase‐inhibitor hydrolysates obtained from ‘in vitro’ enzymatic hydrolysis of mannoproteins extracted from different strains of yeasts

Relationship Between Total Phenolics Content and Antioxidant Activities of Microalgae Under Autotrophic, Heterotrophic and Mixotrophic Growth

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