Micro-volume spectrophotometer with a slope spectroscopy technique

Broadband cavity enhanced UV-VIS absorption spectroscopy for picolitre liquid samples

“…Measuring the spectral properties of the absorbance of materials has been utilised extensively across the scientific world for purposes varying from determining stellar composition 1 to quantifying nucleic acid concentrations. 2,3 Absorption spectroscopy is label free and universal, 4,5 non-destructive and even removes the need for fluorescent tags, 6 which can be time consuming, resource intensive, and modify the physical-chemical properties of specific analytes. 7,8 Routinely used absorption spectrometers in research environments have evolved from measuring a sample solution in a centimetre sized cuvette with a corresponding volume of ∼1 ml to a ∼1 µl sample.…”