Micro two-dimensional electrophoretic analysis of changes in plasma proteins of next-generation rat of the pregnant rats administered to coplanar PCBs

Abstract

SUMMARYPolychlorinated biphenyl (PCB) 126 or PCB 169 was administered to pregnant rats and kinetic changes in plasma protein spots of their next generation (F2 rats) ages 1, 3, 6, and 15 weeks were observed by micro two-dimensional polyacrylamide gel electrophoresis (M2D-PAGE).In the control group in which age-related changes were observable, changes in the form of the albumin (Alb) spot were recognized at ages 1-3 weeks. Only the Alb spot on the acid side, i.e., non-mercaptoalbumin, was recognized at age 1 week. At age 3 weeks, the Alb spot on the alkaline side, i.e., mercaptoalbumin, also became observable. On the other hand, qualitative changes in the form of Alb, i.e., manifestations of mercaptoalbumin, were recognized at age 1 week by administration of PCB 126. This result suggested that the influence of PCB 126 on the living body is greater than that of PCB 169. In spite of the treatment for toxicity by equivalence of dose control on the basis of toxicity equivalency factor (TEF) 0.1 for PCB 126 (PeCB) and 0.01 for PCB 169 (HxCB), the influence of PCB 126 on the living body was greater than that of PCB 169.The C3 spot was increased at age 3 weeks in the PCB 126 and the PCB 169. The spot showed a tendency toward increase at age 6 weeks in the PCB 126. The TEF compared PCB169 with PCB126, and distinctly increase of the C3 spot was observed for 15 weeks though the TEF was low. From these, as for the PCB 169, it was suggested appearing remarkably in aging influence of the immunity toxicity.The observations of the changes in these spots led to the conclusion that the changes in these spots resulted from transfer of the drugs accumulating in the maternal body to the F2 rats of the maternal rats administered PCB 126 and those administered PCB 169 via the breast milk and that these changes are involved with inflammatory proteins, including the target genes influenced by the toxicity of coplanar PCBs.

Analysis of plasma protein in newborn rats treated with diethylstilbestrol by using rapid micro two-dimensional electrophoresis

“…The fact that mercaptoalbumin has increased suggests a rise in mercaptoalbumin biosynthesis in accordance with DES administration 7) . Similar results have also been reported in rats administered polychlorinated biphenyls (PCBs) 10) . Specifically, PCB126 has been confirmed to increase mercaptoalbumin to a greater degree than PCB169, and to have a greater effect in vivo.…”

“…As most proteins in plasma are synthesized in the liver, it is conceivable that DES disrupts AUG synthesis in the liver 4) . Intake of exogenous chemicals, such as dioxins, has also been reported to reduce AUG 10) , but this is believed to be the result of AUG consumption and depletion owing to promotion of retinol metabolism through similar administration. In both cases, the decrease in AUG affects the development of the genitals.…”