Micro Thin-Layer Chromatography Fingerprints of Selected Basil Species and Their Chemometric Analysis

Hawrył, Mirosław; Świeboda, Ryszard; Hawrył, Anna; Niemiec, Małgorzata; Waksmundzka‐Hajnos, Monika

doi:10.5740/jaoacint.17-0166

Cited by 3 publications

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“…The genus Ocimum includes some of the world's most popular herbs, of which there are large numbers of cultivars that differ in their chemical compositions and morphological characteristics 1‐5 . Sweet basil ( Ocimum basilicum L.) is an herbaceous annual plant with a distinct balsamic and spicy aroma, cultivated both industrially and in private households 6 . There are dozens of subspecies and cultivars of sweet basil, mostly distributed in tropical and subtropical regions 1,3,5,6 .…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] 5 Sweet basil (Ocimum basilicum L.) is an herbaceous annual plant with a distinct balsamic and spicy aroma, cultivated both industrially and in private households. 6 There are dozens of subspecies and cultivars of sweet basil, mostly distributed in tropical and subtropical regions. 1,3,5,6 Sweet basil leaves are used in the food industry as flavor and aroma enhancers and are also used in the cosmetics industry.…”

Section: Introductionmentioning

confidence: 99%

“…6 There are dozens of subspecies and cultivars of sweet basil, mostly distributed in tropical and subtropical regions. 1,3,5,6 Sweet basil leaves are used in the food industry as flavor and aroma enhancers and are also used in the cosmetics industry. Sweet basil contains high concentrations of polyphenolic compounds that exhibit antioxidant and antiinflammatory activity and have been used to treat various conditions such as headaches, cough and diarrhea.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of sweet basil harvest time and cultivar characterization using near‐infrared spectroscopy, liquid and gas chromatography, and chemometric statistical methods

et al. 2021

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BACKGROUND: Terpene, eugenol and polyphenolic contents of basil are major determinants of quality, which is affected by genetics, weather, growing practices, pests and diseases. Here, we aimed to develop a simple predictive analytical method for determining the polyphenol, eugenol and terpene content of the leaves of major Israeli sweet basil cultivars grown hydroponically, as a function of harvest time, through the use of near-infrared (NIR) spectroscopy, liquid/gas chromatography, and chemometric methods. We also wanted to identify the harvest time associated with the highest terpene, eugenol and polyphenol content.RESULTS: Six different cultivars and four different harvest times were analyzed. Partial least square regression (PLS-R) analysis yielded an accurate, predictive model that explained more than 93% of the population variance for all of the analyzed compounds. The model yielded good/excellent prediction (R 2 > 0.90, R 2 cv and R 2 pre > 0.80) and very good residual predictive deviation (RPD > 2) for all of the analyzed compounds. Concentrations of rosmarinic acid, eugenol and terpenes increased steadily over the first 3 weeks, peaking in the fourth week in most of the cultivars. Our PLS-discriminant analysis (PLS-DA) model provided accurate harvest classification and prediction as compared to cultivar classification. The sensitivity, specificity and accuracy of harvest classification were larger than 0.82 for all harvest time points, whereas the cultivar classification, resulted in sensitivity values lower than 0.8 in three cultivars. CONCLUSION:The PLS-R model provided good predictions of rosmarinic acid, eugenol and terpene content. Our NIR coupled with a PLS-DA demonstrated reasonable solution for harvest and cultivar classification.

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Section: Introductionmentioning

confidence: 99%