Micro Satellite Markers Screening for Coffee Berry Disease (Colletotrichum Kahawae) Resistance in Progenies of Variety Kp423 and Ethiopian Coffee Arabica Accessions

Detached berries response to coffee berry disease (Colletotrichum kahawae) of F1 hybrid genotypes developed from Ethiopian accessions of Arabica coffee

“…Data were subjected to analysis of variance (ANOVA) for determination of statistical difference using GenStat software (16 th version, VSN International) and means of disease severity were separated using Duncan Multiple Range Test. Resistance categories were then classified using disease incidence as; resistant (R) 0 -25, moderately resistant (MR) 26 -50, moderately susceptible (MS) 51 -75) and susceptible (S) 76 -100 as applied by Mtenga (2006). Disease incidence was performed as per Marasas et al (1988) formula.…”

“…To date, fifteen improved tall and four compact Arabica varieties have been introduced, with additional varieties in the development pipeline (Kilambo et al, 2014). These varieties were derived from a limited number of resistant coffee varieties present in TaCRI germplasm, specifically Catimors, Hibrido de Timor (HdT), and Rume Sudan, which are recognized as progenitors of CBD and CLR resistance (Mtenga et al, 2008). Since the 1950s, genetic material from 2000 Arabica accessions has been utilized to create CBDresistant varieties (TaCRI, 2003).…”

“…Therefore, there is a potential risk in developing CBDresistant varieties without subjecting accessions to the most aggressive strains/isolates, which might lead to the emergence of weaker varieties. Consequently, in breeding programs, it is imperative to carefully select the most suitable accessions within varieties to develop superior coffee strains exhibiting CBD resistance (Mtenga and Reuben, 2012). The objective of this study was to assess the level of CBD resistance within F1 genotypes, thereby identifying the top-performing hybrids.…”