Micro Ribonucleic Acid Combined with Nanoparticles in Molecular Diagnosis of Depression

Abstract

This study was aimed to investigate the application of micro ribonucleic acid (miRNA) combined with nanographene particles in molecular diagnosis of depression. After the sheet-shaped graphene oxide was mixed with a solvent to form a solution, it was atomized at high temperature to form droplets and extract nanographene oxide, which was applied to an electrochemical sensor. 90 patients with depression were selected as the research objects and they were randomly divided into control group and experimental group, patients from the two groups received the miRNA molecular diagnosis and the miRNA molecular diagnosis combined with nanographene electrochemical sensor dopamine detection method, respectively. As a result, the diagnostic sensitivity (82.4%), specificity (90.3%), and accurate index (71.3%) of patients from the experimental group were higher obviously than those of the control group (54.3%, 72.8%, and 55.6%) (P <0.05). The expression levels of MiR-92b-5p and MiR-19a-3p in patients with depression were higher markedly than the levels of the normal population (P <0.05). The detection rates of mild depression, moderate depression, and severe depression in patients from the experimental group were 97.3%, 96.5%, and 91.2%, which were higher greatly than the rates of the control group (84.3%, 85.4%, and 83.2%) (P <0.05). Besides, the false positive rate (11.2%) and false negative rate (5.6%) of patients from the experimental group were sharply lower than the rates of the control group (31.5% and 16.4%) (P <0.05). In short, miRNA combined with nanographene electrochemical sensors could accurately diagnose depression with high accuracy and sensitivity, which could be applied in the clinical diagnosis.