Micro-rheological changes during experimental acute pancreatitis in the rat

Abstract

Although microcirculatory disturbances play pivotal role in the pathomechanism of acute pancreatitis (AP), very few papers can be found which had been tested any of hemorheological parameters. The aim of our study was to analyze the hemorheological changes in cerulein-induced experimental acute pancreatitis in rat in two doses (5 and 10 g/kg, s.c.). Male and female rats were subjected to Control group, or AP with 5 or 10 g/kg cerulein groups. Blood samplings (lateral caudal vein) were completed before cerulein administration, and 1, 2 and 24 hours later. Hematological parameters, amylase activity, erythrocyte deformability (ektacytometry) and aggregation (light-transmission method) were tested. The presence of AP could be confirmed by amylase testing and histological examination. The earliest impairment of the red blood cell deformability could be observed 1 hour after cerulein administration in 10 g/kg dosage. Female animals had the worst rheological results with high mortality. In conclusion, subcutaneously administrated cerulein in dosage of 5 and 10 g/kg resulted in AP in rats, with significant changes in red blood cell deformability and alterations in erythrocyte aggregation. This model seems to be suitable for further comparative studies.

Exercise hemorheology: Classical data, recent findings and unresolved issues

“…A growing interest has been recently devoted to the study of RBC aggregation and its impact on hemodynamics [5]. In addition, several studies reported association between RBC aggregation abnormalities and diseases, probably as a consequence of inflammation and/or metabolic disturbances, such as in primary open-angle glaucoma [56], type 2 diabetes [75], insulin resistance [15,16], sickle cell disease [49,83,93] and others [32,48,58,79,95]. Few studies have investigated RBC aggregation in response to exercise and contrasting results have been reported: some studies described no change [25,84,89], an increase, in association or not with a rise in plasma fibrinogen level [18,36,84], or a delayed decrease [98] with exercise.…”

Data reduction methods for ektacytometry in clinical hemorheology

“…That is, one is not sure at which SS to compare and whether to focus on low, medium or high SS. A single index reflecting RBC deformability would be useful, in experimental and clinical studies [7,12,14,15,22]. Various approaches have been suggested to derive indices of RBC deformability based on EI-SS curves [4,6,7,19], some being implemented into software programs that are part of commercial instruments (e.g., LORCA).…”