Micro-Raman spectroscopy as an enabling tool for long-term intracellular studies of nanomaterials at nanomolar concentration levels

Ignatova, Tetyana; Chandrasekar, Swetha; Pirbhai, Massooma; Jedlicka, Sabrina S.; Rotkin, Slava V.

doi:10.1039/c7tb00766c

Cited by 9 publications

(12 citation statements)

References 91 publications

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“…The average length of carbon nanotubes in our sample is 166 nm . We estimate that the average mass of a nanotube of this length (using the (6,5) species) is 3 × 10 –19 g, based on previous calculations . Thus, for one nanotube, we calculate that approximately 180 molecules of doxorubicin bound strongly.…”

Section: Results and Discussionmentioning

confidence: 90%

“…44 We estimate that the average mass of a nanotube of this length (using the (6,5) species) is 3 × 10 −19 g, based on previous calculations. 45 treatment with buffer, 500 μM Dox, or 500 μM doxorubicin followed by three washes, to assess the impact of washing on the nanotube optical response to doxorubicin (Figure S6c). We observed that the emission spectrum was largely unaltered by washing as compared to the sample containing 500 μM doxorubicin without pelleting and washing (Figure S6d).…”

Section: Nano Lettersmentioning

confidence: 99%

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An in Vivo Nanosensor Measures Compartmental Doxorubicin Exposure

et al. 2019

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Preclinical measurements of drug exposure to specific organs and tissues is normally performed by destructive methods. Tissue-specific measurements are important, especially for drugs with intractable dose-limiting toxicities, such as doxorubicin-mediated cardiotoxicity. We developed a method to rapidly quantify doxorubicin exposure to tissues within living organisms using an implantable optical nanosensor that can be interrogated noninvasively following surgical implantation. The near-infrared fluorescence of single-walled carbon nanotubes functionalized with DNA was found to respond to doxorubicin via a large and uniform red-shift. We found this to be common to DNA-intercalating agents, including anthracycline compounds such as doxorubicin. Doxorubicin was measured in buffer and serum, intracellularly, and from single nanotubes on a surface. Doxorubicin adsorption to the DNA-suspended nanotubes did not displace DNA but bound irreversibly. We incorporated the nanosensors into an implantable membrane which allowed cumulative detection of doxorubicin exposure in vivo. On implanting the devices into different compartments, such as subcutaneously and within the peritoneal cavity, we achieved real-time, minimally invasive detection of doxorubicin injected into the peritoneal cavity, as well as compartment-specific measurements. We measured doxorubicin translocation across the peritoneal membrane in vivo. Robust, minimally invasive pharmacokinetic measurements in vivo suggest the suitability of this technology for preclinical drug discovery applications.

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Section: Results and Discussionmentioning

confidence: 90%

Section: Nano Lettersmentioning

confidence: 99%

An in Vivo Nanosensor Measures Compartmental Doxorubicin Exposure

et al. 2019

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“…In addition, the Raman spectrum was collected using confocal micro-Raman spectroscopy (WITec Control FIVE software). [86] The single-point spectrum was collected with an integration time of 0.1 s.…”

Section: Methodsmentioning

confidence: 99%

“…AFM was performed in tapping mode on dry samples with an AFM tip from Nanoworld: NCST, silicon, f o = 160 kHz, C = 7.4 N m −1 ). In addition, the Raman spectrum was collected using confocal micro‐Raman spectroscopy (WITec Control FIVE software) . The single‐point spectrum was collected with an integration time of 0.1 s.…”

Section: Methodsmentioning

confidence: 99%

Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA‐Wrapped Single‐Walled Carbon Nanotubes

et al. 2019

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Nanostructured biomaterials have been extensively explored in clinical imaging and in gene/drug delivery applications. However, limited studies have been performed that examine the influence that nanomaterials may have on cell behavior over long time scales at nonlethal concentrations. The study was designed to investigate whether carbon nanotubes are able to augment cell behavior at low concentrations. Single-walled carbon nanotubes were introduced to neural stem cells at different stages of differentiation at concentrations as low as 5 ng/mL. Results demonstrate that in this particular cell model, nanotube uptake is mediated by endocytosis.Differentiation is augmented, especially when nanotubes are introduced to cells in an actively dividing state. Significant increases in neuronal cell population were observed over the control specimens. While the mechanisms behind this observation are yet unknown, the study demonstrates that low concentrations of internalized nanomaterials can significantly alter the differentiation profile of a stem cell line.

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“…Such a functionalization facilitates NT entry via endocytosis upon introduction in vitro ( 10 , 12 ) and preserves NT material dispersal in the form of individual tubes, as opposed to some earlier works ( 9 , 13 , 14 , 15 , 16 ). Biophysical characterization of the SWCNTs used can be found in our previous publications ( 17 , 18 ). Our previous studies in C17.2 neural progenitor cells (NPCs) allowed us to define the acceptable range of DNA-SWCNT concentrations (10 pM or lower) to be used for biological applications.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells

Chandrasekar

Kuipa²,

Vargas³

et al. 2022

Biophysical Reports

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Micro-Raman spectroscopy as an enabling tool for long-term intracellular studies of nanomaterials at nanomolar concentration levels

Abstract: Imaging of individual SWCNTs inside neural stem cells has been demonstrated using confocal scanning Raman microscopy. Hyperspectral Raman imaging allowed detection of nanomaterials applied to the cell in ultra-low doses in long-term studies.

Cited by 9 publications

References 91 publications

An in Vivo Nanosensor Measures Compartmental Doxorubicin Exposure

An in Vivo Nanosensor Measures Compartmental Doxorubicin Exposure

Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA‐Wrapped Single‐Walled Carbon Nanotubes

Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells

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