Micro‐PIXE in ecophysiology

Przybyłowicz, W.J.; Mesjasz‐Przybyłowicz, Jolanta; Migula, P.; Nakonieczny, Mirosław; Augustyniak, Maria; Tarnawska, Monika; Turnau, Katarzyna; Ryszka, Przemysław; Orłowska, Elżbieta; Zubek, Szymon; Głowacka, E.

doi:10.1002/xrs.826

Cited by 32 publications

(40 citation statements)

References 19 publications

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“…The normalization of results was done using the integrated beam charge, collected simultaneously from a Faraday cup located behind the specimen and from the insulated specimen holder. A more detailed description of the nuclear microprobe setup at iThemba LABS can be found in Prozesky et al, (1995) and Przybylowicz et al (1999Przybylowicz et al ( , 2005.…”

Section: Instrumentation and Analytical Methodsmentioning

confidence: 99%

Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro‐PIXE

et al. 2008

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Summary• Localization of cadmium (Cd) and other elements was studied in the leaves of the field-collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro-PIXE (proton-induced X-ray emission).• The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole-leaf cross-sections and selected tissues were obtained.• Cd was preferentially localized in the lower epidermis (820 µg g -1 DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll.• Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.

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Section: Instrumentation and Analytical Methodsmentioning

confidence: 99%

Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro‐PIXE

et al. 2008

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“…The normalization of results was done using the integrated beam charge, collected simultaneously from a Faraday cup located behind the specimen and from the insulated specimen holder. A more detailed description of the nuclear microprobe set-up at iThemba LABS can be found in Prozesky et al, (1995) and Przybylowicz et al, (1999Przybylowicz et al, ( , 2001Przybylowicz et al, ( , 2005.…”

Section: Elemental Analysis and Data Evaluationmentioning

confidence: 99%

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

Vardien

Mesjasz‐Przybyłowicz

Przybyłowicz

et al. 2014

Journal of Plant Physiology

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Legumes have the unique ability to fix atmospheric nitrogen (N 2 ) via symbiotic bacteria in their nodules but depend heavily on phosphorus (P), which affects nodulation, and the carbon costs and energy costs of N 2 fixation. Consequently, legumes growing in nutrient-poor ecosystems (e.g. sandstone derived soils) have to enhance P recycling and/ or acquisition in order to maintain N 2 fixation. In this study, we investigated the flexibility of P recycling and distribution within the nodules and their effect on N nutrition in Virgilia divaricata Adamson, Fabaceae, an indigenous legume in the Cape Floristic Region of South Africa. Specifically, we assessed tissue elemental localization using micro-particle induced x-ray emission 1 (PIXE), measured N fixation using nutrient concentrations derived from inductively coupled mass spectrometry (ICP-MS), calculated nutrient costs, and determined P recycling from enzyme activity assays. Morphological and physiological features characteristic of adaptation to P-deprivation were observed for V. divaricata. Decreased plant growth and nodule production with parallel increased root: shoot ratios are some of the plastic features exhibited in response to P deficiency. Plants resupplied with P resembled those supplied with optimal P levels in terms of growth and nutrient acquisition. Under low P conditions, plants maintained an increase in N 2 -fixing efficiency despite lower levels of orthophosphate (Pi) in the nodules.This can be attributed to two factors: i) an increase in Fe concentration under low P, and ii) greater APase activity in both the roots and nodules under low P. These findings suggest that V. divaricata is well-adapted to acquire N under P deficiency, owing to the plasticity of its nodule physiology.

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“…The total accumulated charge per scan varied from 0.3 to 5 mC. A more detailed description of the nuclear microprobe set-up at iThemba LABS can be found in Prozesky et al (1995) and Przybylowicz et al (1999Przybylowicz et al ( , 2001Przybylowicz et al ( , 2005.…”

Section: Elemental Microanalysismentioning

confidence: 99%

Micro‐particle‐induced X‐ray emission mapping of elemental distribution in roots of a Mediterranean‐type sclerophyll, Agathosma betulina (Berg.) Pillans, colonized by Cryptococcus laurentii

Cloete

Przybyłowicz

Mesjasz‐Przybyłowicz

et al. 2010

Plant Cell & Environment

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The role of rhizosphere yeasts as plant nutrient-scavenging microsymbionts in resource-limited Mediterranean-type heathlands is unknown. This study, therefore, focused on quantitative elemental distribution within the roots of a medicinal sclerophyll, Agathosma betulina (Berg.) Pillans, grown under nutrient-poor conditions, and colonized by Cryptococcus laurentii. Micro-particle-induced X-ray emission (PIXE) was used to assess quantitative elemental distribution within the roots of A. betulina inoculated with viable C. laurentii, as well as within roots of control plants that received autoclaved yeast. To aid in the interpretation of heterogeneous elemental distribution patterns, apoplastic barriers (Casparian bands) in root tissues were located using fluorescence microscopy. In addition, root crosssections were examined for endophytic C. laurentii using light and transmission electron microscopy (TEM). The average concentrations of P, Fe and Mn were significantly (P < 0.05) higher in roots of yeast-inoculated plants, compared to control plants. Casparian bands were observed in the exodermal cells of both treatments, and the presence of these bands was correlated with elemental enrichment in the epi/exodermal-outer cortical tissues. Light and TEM micrographs revealed that the yeast was not a root endophyte. This is the first report describing the role of a soil yeast as a plant nutrient-scavenging microsymbiont.

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Micro‐PIXE in ecophysiology

Cited by 32 publications

References 19 publications

Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro‐PIXE

Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro‐PIXE

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

Micro‐particle‐induced X‐ray emission mapping of elemental distribution in roots of a Mediterranean‐type sclerophyll, Agathosma betulina (Berg.) Pillans, colonized by Cryptococcus laurentii

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