Micro‐Patterning of Polydiacetylene Supramolecules using Micromolding in Capillaries (MIMIC)

Baek, Ji Hyun; Ahn, Heejoon; Yoon, Jonghun; Kim, Jong-Man

doi:10.1002/marc.200700693

Cited by 24 publications

(21 citation statements)

References 55 publications

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“…65 A schematic representation of the MIMIC 66 procedure employed for this purpose is given in Figure 7. A polydimethylsiloxane (PDMS) mold was treated with UV/ozone (UVO) to …”

Section: Patterned Fluorescence Imagesmentioning

confidence: 99%

Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors

2008

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This Account describes a new strategy for the preparation of label-free sensor systems based on the fluorogenic properties of the conjugated polymer, polydiacetylene (PDA). PDA has been extensively investigated as a sensor matrix, owing to a brilliant blue-to-red color transition that takes place in response to environmental perturbations. It has been known for some time that "blue-phase" PDAs are nonfluorescent while their "red-phase" counterparts fluoresce. For the most part, however, the significance of the different fluorogenic properties of PDAs has been ignored in the context of sensor applications. In the course of developing PDA-based sensors, we discovered that PDA vesicles can be readily immobilized on solid substrates. This is an attractive property of PDAs since it leads to the combined advantages of the vesicle sensors (which have three-dimensional interactions between sensor and target molecules) and film sensors (which are applicable to a two-dimensional array or chip format). Stable blue-phase immobilized PDAs can be prepared by employing one of three strategies involving the formation of covalent adducts, biotin-avidin complexes, or complexes formed through nonspecific physical adsorption. A procedure for generating well-patterned fluorescence images is necessary for the immobilized PDAs to function in chip-based sensor systems. Patterned fluorescence images are readily constructed by employing (1) the photolithographic technique, (2) the micromolding in capillaries (MIMIC) method, or (3) an array spotting system. Heat treatment of the patterned "blue-phase" PDA vesicles transforms the nonfluorescent images into their fluorescent red forms. The observation that finely resolved fluorescence patterns can be generated by heat treatment of microarrayed PDAs is highly significant in that it indicates that fluorescence signals might be produced by specific molecular recognition events. Indeed, red fluorescence emission is observed when immobilized PDAs are subjected to specific molecular recognition events, such as ligand--cyclodextrin or protein-protein interactions. The facile immobilization of PDA vesicles on solid substrates and the affinity-induced fluorescence emission combine to make this system applicable to the fabrication of label-free PDA sensors. Since in theory any molecular recognition event that promotes the blue-to-red color transition of PDAs should result in the generation of fluorescence, it should be possible to reformat a variety of previously described colorimetric PDA sensors into fluorescence-based sensor systems. The fluorescence properties of PDAs, when combined with modern methods for the fabrication of microarrays, should stimulate the development of a number of new label-free chemosensor systems.

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“…65 A schematic representation of the MIMIC 66 procedure employed for this purpose is given in Figure 7. A polydimethylsiloxane (PDMS) mold was treated with UV/ozone (UVO) to …”

Section: Patterned Fluorescence Imagesmentioning

confidence: 99%

Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors

2008

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“…The patterned prepolymers are subsequently fixed using heat or light. MIMIC can generate features with cross sectional areas down to 0.2 μm 2 with quasi-3D structure in a variety of polymeric materials (e. g., polyurethane, [15] polydiacetylene, [16] polystyrene, [17,18] etc.). MIMIC, however, cannot generate discontinuous networks, and capillaries longer than 1 cm in length are difficult to fill due to viscous drag of the fluid.…”

Section: Established Soft Lithographic Methodsmentioning

confidence: 99%

Emergent Soft Lithographic Tools for the Fabrication of Functional Polymeric Microstructures

2019

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Polymeric microstructures (PMs) are useful to a broad range of technologies applicable to, for example, sensing, energy storage, and soft robotics. Due to the diverse application space of PMs, many techniques (e. g., photolithography, 3D printing, micromilling, etc.) have been developed to fabricate these structures. Stemming from their generality and unique capabilities, the tools encompassed by soft lithography (e. g., replica molding, microcontact printing, etc.), which use soft elastomeric materials as masters in the fabrication of PMs, are particularly relevant. By taking advantage of the characteristics of elastomeric masters, particularly their mechanical and chemical properties, soft lithography has enabled the use of non‐planar substrates and relatively inexpensive equipment in the generation of many types of PMs, redefining existing communities and creating new ones. Traditionally, these elastomeric masters have been produced from relief patterns fabricated using photolithography; however, recent efforts have led to the emergence of new methods that make use of masters that are self‐forming, dynamic in their geometric and chemical properties, 3D in architecture, and/or sacrificial (i. e., easily removed/released using phase changes). These “next generation” soft lithographic masters include self‐assembled liquid droplets, microscale balloons, templates derived from natural materials, and hierarchically microstructured surfaces. The new methods of fabrication supported by these unique masters enable access to numerous varieties of PMs (e. g., those with hierarchical microstructures, overhanging features, and 3D architectures) that would not be possible following established methods of soft lithography. This review explores these emergent soft lithographic methods, addressing their operational principles and the application space they can impact.

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“…Depending on the configuration of the mold, it is possible to produce structures of variable size and thickness in a single step. The technique has been shown to be compatible with a broad range of materials, including organic molecules [65][66][67], prepolymers [68], colloidal particles [69,70], and biological macromolecules [71,72]. Submicrometer patterning, however, is challenging since resistance to flow increases at smaller scales, which makes it difficult for liquid to fill the capillaries, especially over long distances.…”

Section: Patterning Based On Moldingmentioning

confidence: 99%

Sub-Micrometer Patterning Using Soft Lithography

Singh¹,

Geißler²

2017

Reference Module in Materials Science and Materials Engineering

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Micro‐Patterning of Polydiacetylene Supramolecules using Micromolding in Capillaries (MIMIC)

Cited by 24 publications

References 55 publications

Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors

Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors

Emergent Soft Lithographic Tools for the Fabrication of Functional Polymeric Microstructures

Sub-Micrometer Patterning Using Soft Lithography

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