Micro-magnet arrays for specific single bacterial cell positioning

Pivetal, Jérémy; Royet, David; Ciuta, Georgeta; Frénéa‐Robin, Marie; Haddour, Naoufel; Dempsey, Nora; Dumas-Bouchiat, Frédéric; Simonet, Pascal

doi:10.1016/j.jmmm.2014.09.068

Cited by 30 publications

(18 citation statements)

References 45 publications

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“…Magnetic arrays are suitable for bacteria patterning despite the fact that bacteria cells are smaller than mammalian cells. Pivetal et al [ 44 ] fabricated, by using reversed magnetization with thermomagnetic patterning, a patterned array of 7.5 × 7.5 mm 2 micromagnets. Bacteria were labelled magnetically by immunomagnetic in situ hybridization to increase specificity and guarantee bacteria fixation ( Figure 4 ).…”

Section: Techniques and Methodsmentioning

confidence: 99%

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

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Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalized medicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.

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Section: Techniques and Methodsmentioning

confidence: 99%

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

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“…Under this configuration, the solution containing viral product can be concentrated within 20 min and yields a solution that is 40-80 times more concentrated in comparison with the initial solution [14]. On the other hand, Pivetal and coworkers designed a micro-magnet array which can develop magnetic field gradient as high as 2.5 Â 10 5 T m 21 to position and immobilize magnetic particle-labelled E. coli (row 11 in table 1) [57]. Meanwhile, there are plenty of biomedical diagnostic applications which have employed the concept of LGMS in their operation unwittingly and resulted in positive outcomes.…”

Section: Application Of High and Low Gradient Magnetic Separations Inmentioning

confidence: 99%

Working principle and application of magnetic separation for biomedical diagnostic at high- and low-field gradients

2016

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Magnetic separation is a versatile technique used in sample preparation for diagnostic purpose. For such application, an external magnetic field is applied to drive the separation of target entity (e.g. bacteria, viruses, parasites and cancer cells) from a complex raw sample in order to ease the subsequent task(s) for disease diagnosis. This separation process not only can be achieved via the utilization of high magnetic field gradient, but also, in most cases, low magnetic field gradient with magnitude less than 100 T m is equally feasible. It is the aim of this review paper to summarize the usage of both high gradient magnetic separation and low gradient magnetic separation (LGMS) techniques in this area of research. It is noteworthy that effectiveness of the magnetic separation process not only determines the outcome of a diagnosis but also directly influences its accuracy as well as sensing time involved. Therefore, understanding the factors that simultaneously influence the efficiency of both magnetic separation process and target detection is necessary. Moreover, for LGMS, there are several important considerations that should be taken into account in order to ensure its successful implementation. Hence, this review paper aims to provide an overview to relate all this crucial information by linking the magnetic separation theory to biomedical diagnostic applications.

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“…The possibility of using nanomagnets with high stray field gradient in self-assembling process was further elaborated in several studies. [12][13][14][15][16] Thus in Ref. the authors investigated the assembly of magnetotactic bacteria and magnetite or cobaltite nanoparticles on micron-sized periodic patterns of commercial audio tapes.…”

Section: Introductionmentioning

confidence: 99%

Design of Intense Nanoscale Stray Fields and Gradients at Magnetic Nanorod Interfaces

Ivanov

Leliaert

Crespo

et al. 2019

ACS Appl. Mater. Interfaces

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We explore electrodeposited ordered arrays of Fe, Ni and Co nanorods embedded in anodic alumina membranes as a source of intense magnetic stray field gradients localized at the nanoscale. We perform a multiscale characterization of the stray fields using a combination of experimental methods (Magneto-optical Kerr effect, Virtual Bright Field Differential Phase Contrast Imaging) and micromagnetic simulations, and establish a clear correlation between the stray fields and the magnetic configurations of the nanorods. For uniformly magnetized Fe and Ni wires the field gradients vary following Page 2 of 33 ACS Paragon Plus Environment ACS Applied Materials & Interfaces 3 saturation magnetization of corresponding metal and the diameter of the wires. In the case of Co nanorods, very localized (~10 nm) and intense (> 1T) stray field sources are associated with the cores of magnetic vortexes. Confinement of that strong field at extremely small dimensions leads to exceptionally high field gradients up to 10 8 T/m. These results demonstrate a clear path to design and fine-tune nanoscale magnetic stray field ordered patterns with a broad applicability in key nanotechnologies, such as nanomedicine, nanobiology, nanoplasmonics and sensors.

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Micro-magnet arrays for specific single bacterial cell positioning

Cited by 30 publications

References 45 publications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Working principle and application of magnetic separation for biomedical diagnostic at high- and low-field gradients

Design of Intense Nanoscale Stray Fields and Gradients at Magnetic Nanorod Interfaces

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