Micro-interferometry of Insect Polyploid Nuclei

Mello, Maria Luiza S.

doi:10.1508/cytologia.37.261

Cited by 3 publications

(1 citation statement)

References 15 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When these distances continue to increase or even decrease in adult specimens, such factors as a variable presence of nonhistone proteins and/or rRNA transcripts due to functional requirements may be responsible. Variable hydration levels are also known to contribute to the nuclear volume (Mello, 1972).…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

et al. 2020

Self Cite

View full text Add to dashboard Cite

Constitutive heterochromatin typically exhibits low gene density and is commonly found adjacent or close to the nuclear periphery, in contrast to transcriptionally active genes concentrated in the innermost nuclear region. In Triatoma infestans cells, conspicuous constitutive heterochromatin forms deeply stained structures named chromocenters. However, to the best of our knowledge, no information exists regarding whether these chromocenters acquire a precise topology in the cell nuclei or whether their 18S rDNA, which is important for ribosome function, faces the nuclear center preferentially. In this work, the spatial distribution of fluorescent Feulgen-stained chromocenters and the distribution of their 18S rDNA was analyzed in Malpighian tubule cells of T. infestans using confocal microscopy. The chromocenters were shown to be spatially positioned relatively close to the nuclear periphery, though not adjacent to it. The variable distance between the chromocenters and the nuclear periphery suggests mobility of these bodies within the cell nuclei. The distribution of 18S rDNA at the edge of the chromocenters was not found to face the nuclear interior exclusively. Because the genome regions containing 18S rDNA in the chromocenters also face the nuclear periphery, the proximity of the chromocenters to this nuclear region is not assumed to be associated with overall gene silencing.

show abstract

Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Genetics of Bees Other than Apis mellifera

Moritz¹

1986

Bee Genetics and Breeding

View full text Add to dashboard Cite

Effect of temperature on dry mass of polytene nuclei in Drosophila

Hartmann-Goldstein

Goldstein

1979

Journal of Cell Science

View full text Add to dashboard Cite

Nuclei were isolated by an aqueous detergent method from Drosophila prepupal salivary glands, and measured by integrating interference microscopy. There was a highly significant correlation between nuclear volume and dry mass. Dry masses fell into 2, 3 or 4 distinct groups corresponding to polytene replication classes; the mean of a given dry mass group was between 8 and 30% less than twice that of the group below, indicating that the ratio of DNA:dry mass increases during polytenic growth. The proportion of nuclei in the higher mass groups, the mean dry mass of nuclei within a given mass group, and the percentage loss of nuclear dry mass in the first hour after isolation were all higher when animals were reared at 15 degrees instead of 25 degrees C. Nuclear dry mass in prepupae was affected by the temperature during both the embryonic and larval periods, and also to some extent by the nutrition and degree of crowding of the cultures.

show abstract

Micro-interferometry of Insect Polyploid Nuclei

Cited by 3 publications

References 15 publications

Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

Genetics of Bees Other than Apis mellifera

Effect of temperature on dry mass of polytene nuclei in Drosophila

Contact Info

Product

Resources

About