Micro‐instillation of fluorescein with an inoculation loop for ocular surface staining in dry eye syndrome

Courrier, Emilie; Renault, Didier; Kaspi, Mathilde; Marcon, Agathe; Lambert, Victor; Garcin, Thibaud; Chiambaretta, Frédéric; Garhöfer, Gerhard; Thuret, Gilles; Gain, Philippe

doi:10.1111/aos.13559

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…There are several accepted methods for delivering uorescein sodium solution onto the ocular surface when performing uorescein staining. [5] In the current study, we compared standard uorescein strips and a 1 mL syringe method with an innovative newly developed uorescein pen. The standard uorescein strip method cannot administer exact concentrations and quantities.…”

Section: Discussionmentioning

confidence: 99%

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Fluorescein pen: a novel tool with good ease of use for corneal fluorescein staining

Jiang

Wang

Zhang

et al. 2022

Preprint

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Background The purpose of this study was to validate a fluorescein pen for use in corneal staining and to compare this novel technique with two conventional tools. Methods An insulin pen was converted into a fluorescein pen for use in corneal staining. The fluorescein pen was provided to 15 ophthalmologists for evaluating the ease of use as compared with two other tools (standard fluorescein strips and the 1 mL syringe method). Each fluorescein pen was used multiple times and underwent microbiological cultivation weekly. If the microbial culture was negative, the pen was used until the fluorescein in the cartridge was used up. Cornea fluorescein staining with three tools was conducted on 16 patients. Images were taken under slit lamp cobalt blue light to assess image quality and consistency. Results The average application span for the fluorescein pen was 87.7 ± 17.0 days (n = 15). No microbial growth was detected in any sample during the application period. Ophthalmologists’ assessments for ease of use via evaluation scores on a five-point Likert scale were 4.933 ± 0.26, 2.6 ± 0.83, and 2.2 ± 0.68 for the pen, strips, and syringe method, respectively. The fluorescein pen demonstrated greater ease of use than the other two tools (p < 0.0001). Image quality and consistency using the fluorescein pen were improved as compared with the other two tools. Conclusions We conclude that the fluorescein pen has several advantages over traditional staining methods, including greater ease of use, safety for multiple uses, and better yield in terms of improved corneal staining images.

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Section: Discussionmentioning

confidence: 99%

“…The reported concentrations of uorescein used in ocular surface staining differ, ranging from 0.1-1% [5][6][7]. We applied a concentration of 0.5% in our study, because this was the optimal concentration identi ed in our pilot study and because we use this concentration for corneal staining in our practice (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Fluorescein pen: a novel tool with good ease of use for corneal fluorescein staining

Jiang

Wang

Zhang

et al. 2022

Preprint

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“…Raj et al 49 also found a positive correlation between Schirmer's and tear meniscus and concluded that OCT enables a better understanding and treatment of DED. Tear meniscus height measurements obtained by frequency domain OCT and a keratograph were compared by Baek et al 50 and Arriola et al 51 52 presented an all-in-one non-contact ocular surface imaging device (LacryDiag-Quantel Medical) that measures various dry eye parameters that include the tear meniscus height. It also provides lipid layer analysis, non-invasive breakup time (NIBUT), and meibography analysis, which all require manual intervention to obtain the final quantification.…”

Section: Semi-automated Methodsmentioning

confidence: 99%

“…Courrier et al 52 presented an all‐in‐one non‐contact ocular surface imaging device (LacryDiag‐Quantel Medical) that measures various dry eye parameters that include the tear meniscus height. It also provides lipid layer analysis, non‐invasive breakup time (NIBUT), and meibography analysis, which all require manual intervention to obtain the final quantification.…”

Section: Tear Secretion and Volumementioning

confidence: 99%

Automation of dry eye disease quantitative assessment: A review

Brahim

Lamard

Benyoussef

2022

Clinical Exper Ophthalmology

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Dry eye disease (DED) is a common eye condition worldwide and a primary reason for visits to the ophthalmologist. DED diagnosis is performed through a combination of tests, some of which are unfortunately invasive, non‐reproducible and lack accuracy. The following review describes methods that diagnose and measure the extent of eye dryness, enabling clinicians to quantify its severity. Our aim with this paper is to review classical methods as well as those that incorporate automation. For only four ways of quantifying DED, we take a deeper look into what main elements can benefit from automation and the different ways studies have incorporated it. Like numerous medical fields, Artificial Intelligence (AI) appears to be the path towards quality DED diagnosis. This review categorises diagnostic methods into the following: classical, semi‐automated and promising AI‐based automated methods.

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“…In addition, an additional donor cornea was infected in the ASM with the same protocol and observed with an ophthalmologic slit lamp after fluorescein staining [26] (Fluorescein Faure single dose, SERB Laboratories, Paris, France) to show clinician readers the similarity in pattern to dendritic keratitis in vivo.…”

Section: Hsv-1 Infection Of Human Corneal Tissue Ex Vivomentioning

confidence: 99%

Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machine

et al. 2020

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Background Herpetic keratitis (HK) models using whole human corneas are essential for studying virushost relationships, because of high species specificity and the role of interactions between corneal cell populations that cell culture cannot reproduce. Nevertheless, the two current corneal storage methods (hypothermia and organ culture (OC)) do not preserve corneas in good physiological condition, as they are characterized by epithelial abrasion, stromal oedema, and excessive endothelial mortality. Methods To rehabilitate human corneas intended for scientific use, we used an active storage machine (ASM) that restores two physiological parameters that are essential for corneal homeostasis: intraocular pressure and storage medium renewal (21mmHg and 2.6 μL/min, respectively). ASM storage regenerates a normal multilayer epithelium in 2 weeks. We infected six pairs of corneas unsuitable for graft by inoculating the epithelium with herpes simplex virus type 1 (HSV-1), and compared each ASM-stored cornea with the other cornea stored in the same medium using the conventional OC method. Results Only corneas in the ASM developed a dendritic (n = 3) or geographic (n = 2) epithelial ulcer reproducing typical HSV-1-induced clinical lesions. Corneas in OC showed only extensive desquamations. None of the uninfected controls showed epithelial damage. Histology,

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Micro‐instillation of fluorescein with an inoculation loop for ocular surface staining in dry eye syndrome

Abstract: The 1 μl-calibrated inoculation loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.

Cited by 5 publications

References 22 publications

Fluorescein pen: a novel tool with good ease of use for corneal fluorescein staining

Fluorescein pen: a novel tool with good ease of use for corneal fluorescein staining

Automation of dry eye disease quantitative assessment: A review

Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machine

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