Micro Imaging Displays the Sucrose Landscape within and along Its Allocation Pathways

Guendel, André; Rolletschek, Hardy; Wagner, Steffen; Muszyńska, Aleksandra; Borisjuk, Ljudmilla

doi:10.1104/pp.18.00947

Cited by 25 publications

(37 citation statements)

References 45 publications

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“…Baseline features are subtracted at the end of each iteration until the model converges toward a purely chemical spectral data set. Standardization of the component-specific images derived from unique fingerprint features of single (sucrose) or multiple compounds (carbohydrates) enables the quantitative assessment (Gündel et al, 2018). Images were digitally dissected into phloem, cambium, and xylem regions of the hypocotyl cross section.…”

Section: Ftir Data Processingmentioning

confidence: 99%

Clubroot Disease Stimulates Early Steps of Phloem Differentiation and Recruits SWEET Sucrose Transporters within Developing Galls

et al. 2018

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Successful biotrophic plant pathogens can divert host nutrition toward infection sites. Here we describe how the protist Plasmodiophora brassicae establishes a long-term feeding relationship with its host by stimulating phloem differentiation and phloem-specific expression of sugar transporters within developing galls. Development of galls in infected Arabidopsis (Arabidopsis thaliana) plants is accompanied by stimulation of host BREVIS RADIX, COTYLEDON VASCULAR PATTERN, and OCTOPUS gene expression leading to an increase in phloem complexity. We characterized how the arrest of this developmental reprogramming influences both the host and the invading pathogen. Furthermore, we found that infection leads to phloem-specific accumulation of SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS11 and 12 facilitating local distribution of sugars toward the pathogen. Utilizing Fourier-transform infrared microspectroscopy to monitor spatial distribution of carbohydrates, we found that infection leads to the formation of a strong physiological sink at the site of infection. High resolution metabolic and structural imaging of sucrose distributions revealed that sweet11 sweet12 double mutants are impaired in sugar transport toward the pathogen, delaying disease progression. This work highlights the importance of precise regulation of sugar partitioning for plant-pathogen interactions and the dependence of P. brassicae's performance on its capacity to induce a phloem sink at the feeding site.

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Section: Ftir Data Processingmentioning

confidence: 99%

Clubroot Disease Stimulates Early Steps of Phloem Differentiation and Recruits SWEET Sucrose Transporters within Developing Galls

et al. 2018

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“…Such a distinct distribution of disaccharide isomers may suggest their diverse biological function, such as serving as carbon source, regulating protein function and so on. Infrared-based microspectroscopic method have also been utilized in visualizing sucrose distribution in the cereal crop barley and Arabidopsis, but it can't distinguish sucrose from other disaccharide isomers (Guendel, Rolletschek, Wagner, Muszynska, & Borisjuk, 2018). Comparing with the recent report utilizing liquid extraction surface analysis (LESA)-ion mobility mass spectrometry in imaging disaccharide isomers (Nagy, et al, 2019), MALDI-TOF/TOF imaging is superior in lateral resolution and convenience, which could facilitate the study of bacteria-plant interaction in more details.…”

Section: Ms/ms Imaging Of Disaccharides In Other Plant Tissuesmentioning

confidence: 99%

“…Mapping the disaccharide over time and space provide important implications for understanding the adaptive and stress response (Guendel, Rolletschek, Wagner, Muszynska, & Borisjuk, 2018). Moreover, the distribution of carbohydrates can serve as indicators for the flavor of fruits.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the distribution of carbohydrates can serve as indicators for the flavor of fruits. One MSI study found that sucrose are mainly distributed in the upper side of cortical and pith tissue, which indicated top side are sweeter than bottom side of strawberry (Enomoto, Sato, Miyamoto, Ohtsuka, & Yamane, 2018;Guendel, Rolletschek, Wagner, Muszynska, & Borisjuk, 2018). In some reports, the disaccharides in some plant tissues were merely assigned to sucrose, such as Medicago (Ye, Gemperline, Venkateshwaran, Chen, Delaux, Howes-Podoll, et al, 2013), cassava tuber (Li, Knudsen, Hansen, Jorgensen, Kannangara, Bak, et al, 2013), and soybean seeds (Sagara, Bhandari, Spengler, & Vollmann, 2020).…”

Section: Introductionmentioning

confidence: 99%

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MALDI-TOF/TOF tandem mass spectrometry imaging reveals non-uniform distribution of disaccharide isomers in plant tissues

et al. 2021

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Mass spectrometry imaging (MSI) is powerful for investigating the biomolecular locations within tissues. However, the isomeric compounds are rarely distinguished due to inability of MSI to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds. Here we apply MALDI-TOF/TOF tandem mass spectrometry imaging approach to revealing the spatial distributions of isomeric disaccharides in plant tissues. First, the MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, we conducted tandem MS imaging of disaccharides in onion bulb tissues, which indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.

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“…While caryopsis tissue differentiation and cell specifications are crucial during the early grain‐filling period, assimilate supply and distribution is more important in the latter stages. For example, how sugars are spatio‐temporally dispersed in the developing barley grain could be elegantly observed for the first time using latest micro‐imaging technologies (Peukert et al ; Guendel et al ).…”

Section: Promising Research Areas For the Post‐genomics Eramentioning

confidence: 99%

Wheat and barley biology: Towards new frontiers

Schnurbusch

2019

JIPB

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Micro Imaging Displays the Sucrose Landscape within and along Its Allocation Pathways

Cited by 25 publications

References 45 publications

Clubroot Disease Stimulates Early Steps of Phloem Differentiation and Recruits SWEET Sucrose Transporters within Developing Galls

Clubroot Disease Stimulates Early Steps of Phloem Differentiation and Recruits SWEET Sucrose Transporters within Developing Galls

MALDI-TOF/TOF tandem mass spectrometry imaging reveals non-uniform distribution of disaccharide isomers in plant tissues

Wheat and barley biology: Towards new frontiers

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