Micro-Chamber/Thermal Extractor (µ-CTE) as a new sampling system for VOCs emitted by feces

Rațiu, Ileana-Andreea; Mametov, Radik; Ligor, Tomasz; Buszewski, Bogusław

doi:10.1038/s41598-021-98279-z

Cited by 3 publications

(4 citation statements)

References 49 publications

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“…To liberate VOCs from fecal samples the emission chambers system was used-Micro-Chamber/Thermal Extractor™-µ-CTE™ 120 (Markes International, Bridgend, UK). Detailed information about the design and metrological characteristics of the µ-CTE™ 120 system was presented in previous literature [35]. VOCs collection was carried out at nitrogen flow rate of 50 ml min −1 .…”

Section: Instrumentationmentioning

confidence: 99%

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Screening for volatile biomarkers of colorectal cancer by analyzing breath and fecal samples using thermal desorption combined with GC-MS (TD-GC-MS)

Śmiełowska¹,

Ligor

Kupczyk

et al. 2023

J. Breath Res.

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Breath and fecal volatile organic compounds (VOCs), among others, represent a new and encouraging clinical practice for the differential diagnosis of colorectal cancer (CRC). The purpose of our research was to identify VOCs present in exhaled air and feces of 20 healthy volunteers (HV) and 15 CRC patients. For collection of gas phase released from feces, emission microchambers were applied. Sorption tubes were used to enrich analytes for both breath and fecal samples. Thermal desorption (TD) technique combined with gas chromatography coupled with mass spectrometry was used at the separation and identification step. The combination of statistical methods was used to evaluate the ability of VOCs to classify control group and CRC patients. Heptanoic acid, acetone, 2,6,10-trimethyldodecane, n-hexane, skatole, and dimethyl trisulfide are observed in elevated amounts in the patients group. The performance of diagnostic models on the tested data set was above 90%. This study is the first attempt to document the using of TD-GC-MS to analyze both breath and fecal samples to search for volatile biomarkers of CRC. A full evaluation of the results described herein requires further studies involving a larger number of samples. Moreover, it is particularly important to understand the metabolic pathways of substances postulated as tumor biomarkers.

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Section: Instrumentationmentioning

confidence: 99%

“…For the fecal study, a previously optimized protocol was used [35]. Briefly, 250 mg of sample was weighed each time on aluminum dishes.…”

Section: Sample Collectionmentioning

confidence: 99%

Screening for volatile biomarkers of colorectal cancer by analyzing breath and fecal samples using thermal desorption combined with GC-MS (TD-GC-MS)

Śmiełowska¹,

Ligor

Kupczyk

et al. 2023

J. Breath Res.

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“…Recently, the Microchamber was optimized according to number of peaks, peak area and peak intensity [38]. Here, increasing sampling time resulted in an increasing number of peaks, larger peak areas and higher peak intensities.…”

Section: Discussionmentioning

confidence: 99%

The optimization and comparison of two high-throughput faecal headspace sampling platforms: the microchamber/thermal extractor and hi-capacity sorptive extraction probes (HiSorb)

van Vorstenbosch,

Mommers,

Pachen

et al. 2024

J. Breath Res.

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Disease detection and monitoring using Volatile Organic Compounds (VOCs) is becoming increasingly popular. For a variety of (gastrointestinal) diseases the microbiome should be considered. As its output is to large extent volatile, faecal volatilomics carries great potential. One technical limitation is that current faecal headspace analysis requires specialized instrumentation which is costly and typically does not work in harmony with Thermal Desorption units often utilized in e.g. exhaled breath studies. This lack of harmonization hinders uptake of such analyses by the Volatilomics community. Therefore, this study optimized and compared two recently harmonized faecal headspace sampling platforms: High-capacity Sorptive extraction (HiSorb) probes and the Microchamber thermal extractor (Microchamber). Statistical Design of Experiment (DOE) was applied to find optimal sampling conditions by maximizing reproducibility, the number of VOCs detected, and between subject variation. To foster general applicability those factors were defined using semi-targeted as well as untargeted metabolic profiles. HiSorb probes were found to result in a faster sampling procedure, higher number of detected VOCs, and higher stability. The headspace collection using the Microchamber resulted in a lower number of detected VOCs, longer sampling times and decreased stability despite a smaller number of interfering VOCs and no background signals. Based on the observed profiles, recommendations are provided on pre-processing and study design when using either one of both platforms. Both can be used to perform faecal headspace collection, but altogether HiSorb is recommended.

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“…Here, upon storage, the feces was weighed into two aliquots of 250 mg each using sterilized glass vials and kept on ice. Subsequently, their headspace was collected using a MicroChamber-Thermal extractor [ 28 ] (termed Microchamber). The Microchamber consists of 6 heated and purged chambers that offer a dynamic headspace sampling strategy to transfer VOCs into thermal desorption tubes (Tenax/Carbograph-5TD tubes, Markes International Ltd., Llantrisant, UK).…”

Section: Methodsmentioning

confidence: 99%

The Detection of Primary Sclerosing Cholangitis Using Volatile Metabolites in Fecal Headspace and Exhaled Breath

van Vorstenbosch,

van Munster,

Pachen

et al. 2023

Metabolites

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Up to 5% of inflammatory bowel disease patients may at some point develop primary sclerosing cholangitis (PSC). PSC is a rare liver disease that ultimately results in liver damage, cirrhosis and liver failure. It typically remains subclinical until irreversible damage has been inflicted. Hence, it is crucial to screen IBD patients for PSC, but its early detection is challenging, and the disease’s etiology is not well understood. This current study aimed at the early detection of PSC in an IBD population using Volatile Organic Compounds in fecal headspace and exhaled breath. To this aim, fecal material and exhaled breath were collected from 73 patients (n = 16 PSC/IBD; n = 8 PSC; n = 49 IBD), and their volatile profile were analyzed using Gas Chromatography–Mass Spectrometry. Using the most discriminatory features, PSC detection resulted in areas under the ROC curve (AUCs) of 0.83 and 0.84 based on fecal headspace and exhaled breath, respectively. Upon data fusion, the predictive performance increased to AUC 0.92. The observed features in the fecal headspace relate to detrimental microbial dysbiosis and exogenous exposure. Future research should aim for the early detection of PSC in a prospective study design.

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Micro-Chamber/Thermal Extractor (µ-CTE) as a new sampling system for VOCs emitted by feces

Cited by 3 publications

References 49 publications

Screening for volatile biomarkers of colorectal cancer by analyzing breath and fecal samples using thermal desorption combined with GC-MS (TD-GC-MS)

Screening for volatile biomarkers of colorectal cancer by analyzing breath and fecal samples using thermal desorption combined with GC-MS (TD-GC-MS)

The optimization and comparison of two high-throughput faecal headspace sampling platforms: the microchamber/thermal extractor and hi-capacity sorptive extraction probes (HiSorb)

The Detection of Primary Sclerosing Cholangitis Using Volatile Metabolites in Fecal Headspace and Exhaled Breath

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