Micro- and submicroscopic changes of the cerebellar cortex 21 days after modeling the burn

Abstract

The cerebellum is a complexly organized multifunctional component of the brain and a target in numerous lesions, so the study of its morphofunctional state in various pathological conditions and burns does not lose its relevance. The aim of our study was to establish the features of micro- and submicroscopic changes in the structural components of the cerebellar cortex after 21 days under conditions of experimental thermal trauma. The experimental study was simulated on white laboratory male rats. Grade III burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the affected area was 18-20% of the epilated surface of the body of rats. Histological changes were studied for 21 days from the beginning of the experiment. Semi-thin sections for light microscopy were stained with methylene blue, for electron microscopy the obtained ultra-thin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. With the help of micro- and submicroscopic studies, significant alternative changes of both neurons of all layers of the cerebellar cortex and parts of the microcirculatory tract of the organ were revealed. Neurons are disorganized, their shape is changed, there is low functional activity, due to a decrease in the area of the chromatophilic substance. Often there are cell-free areas in the ganglion layer, there is a shift of Purkinje cells deep into the granular layer, and grain cells are pushed sharply into the higher molecular layer. Thus, 21 days after the experimental thermal injury, destructive-degenerative changes of neurons of the cerebellar cortex, paravasal edema and disorders of transendothelial metabolism were established.