Micro- and nanostructured Al2O3 surfaces for controlled vascular endothelial and smooth muscle cell adhesion and proliferation

Aktas, Cenk; Dörrschuck, E.; Schuh, Cathrin; Miró, Marina Martinez; Lee, Juseok; Pütz, Norbert; Wennemuth, Gunther; Metzger, Wolfgang; Oberringer, Martin; Veith, Michael; Abdul‐Khaliq, Hashim

doi:10.1016/j.msec.2012.02.032

Cited by 25 publications

(18 citation statements)

References 22 publications

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“…Unusual cell shape and lack of cell spreading can cause cell death in each of the cell types studied here [ 36 ], which may explain the observed decrease in cell survival and proliferation rate of TNAs. Similar results were also obtained by culturing HUVECs on high-density Al 2 O 3 nanowires [ 37 ] and ZnO nanorods [ 31 ]. Cell processes such as proliferation and apoptosis are arbitrated by cell shape and cytoskeletal organization directly determined by cell/surface interaction [ 38 ].…”

Section: Resultssupporting

confidence: 79%

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Chen¹,

Tang²,

Chen³

et al. 2016

Nanoscale Res Lett

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Carbon plasma nanocoatings with controlled fraction of sp3-C bonding were deposited on TiO2 nanorod arrays (TNAs) by DC magnetic-filtered cathodic vacuum arc deposition (FCVAD). The cytocompatibility of TNA/carbon nanocomposites was systematically investigated. Human umbilical vein endothelial cells (HUVECs) were cultured on the nanocomposites for 4, 24, and 72 h in vitro. It was found that plasma-treated TNAs exhibited excellent cell viability as compared to the untreated. Importantly, our results show that cellular responses positively correlate with the sp3-C content. The cells cultured on high sp3-C-contented substrates exhibit better attachment, shape configuration, and proliferation. These findings indicate that the nanocomposites with high sp3-C content possessed superior cytocompatibility. Notably, the nanocomposites drastically reduced platelet adhesion and activation in our previous studies. Taken together, these findings suggest the TNA/carbon scaffold may serve as a guide for the design of multi-functionality devices that promotes endothelialization and improves hemocompatibility.

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Section: Resultssupporting

confidence: 79%

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Chen¹,

Tang²,

Chen³

et al. 2016

Nanoscale Res Lett

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“…In this context various surface modification methods have been investigated to control the cellular response on stent materials without altering their bulk properties [ 3 , 4 ]. Among other surface properties, the topography plays a major role in the endothelialization as we showed previously [ 5 ]. Nanotopography enhances the adhesion and the proliferation of endothelial cells on the substratum.…”

Section: Introductionmentioning

confidence: 76%

Recombinant Phage Coated 1D Al₂O₃Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells

Lee

Jeon

Haidar

et al. 2015

BioMed Research International

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A novel synthesis of a nanostructured cell adhesive surface is investigated for future stent developments. One-dimensional (1D) Al2O3 nanostructures were prepared by chemical vapor deposition of a single source precursor. Afterwards, recombinant filamentous bacteriophages which display a short binding motif with a cell adhesive peptide (RGD) on p3 and p8 proteins were immobilized on these 1D Al2O3 nanostructures by a simple dip-coating process to study the cellular response of human endothelial EA hy.926. While the cell density decreased on as-deposited 1D Al2O3 nanostructures, we observed enhanced cell proliferation and cell-cell interaction on recombinant phage overcoated 1D Al2O3 nanostructures. The recombinant phage overcoating also supports an isotropic cell spreading rather than elongated cell morphology as we observed on as-deposited Al2O3 1D nanostructures.

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“…9,10 Contact guidance is also an important parameter in the design of medical implant surfaces, as tailored topography can provide a level of cellular control in vivo. 11,12 First observed in 1908, 13 it is now known that contact guidance is conserved across many cell types, 8,[14][15][16][17][18][19] and can be observed both during cell spreading 20,21 and after cells have completely spread. 3,8,19,[22][23][24][25] A number of studies have given insight into the cellular mechanisms underlying contact guidance, with several reporting that the development of internal cell tension is important 3,20,23,24 while others posit that actin polymerization is the driving force.…”

Section: Introductionmentioning

confidence: 99%

Initial contact guidance during cell spreading is contractility-independent

2017

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A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In this study, we investigated contact guidance by studying the reaction of human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial cell spreading. As the cytoskeleton plays a major role in cellular adaptation to topographical features, two methods were used to perturb cytoskeletal structures. Inhibition of actomyosin contractility with the chemical inhibitor blebbistatatin demonstrated that initial contact guidance events are independent of traction force generation. However, cell alignment to the grooved substrate was altered at later time points, suggesting an initial 'passive' phase of contact guidance, followed by a contractility-dependent 'active' phase that relies on mechanosensitive feedback. The actin cytoskeleton was also perturbed in an indirect manner by culturing cells upside down, resulting in decreased levels of contact guidance and suggesting that a possible loss of contact between the actin cytoskeleton and the substrate could lead to cytoskeleton impairment. The process of contact guidance at the microscale was found to be primarily lamellipodia driven, as no bias in filopodia extension was observed on micron-scale grooves.

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Micro- and nanostructured Al2O3 surfaces for controlled vascular endothelial and smooth muscle cell adhesion and proliferation

Cited by 25 publications

References 22 publications

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Recombinant Phage Coated 1D Al₂O₃Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells

Initial contact guidance during cell spreading is contractility-independent

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Micro- and nanostructured Al2O3 surfaces for controlled vascular endothelial and smooth muscle cell adhesion and proliferation

Cited by 25 publications

References 22 publications

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

Recombinant Phage Coated 1D Al2O3Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells

Initial contact guidance during cell spreading is contractility-independent

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Product

Resources

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Recombinant Phage Coated 1D Al₂O₃Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells