Micro- and Nanoscale Imaging of Fluids in Water Using Refractive-Index-Matched Materials

Satake, Shin‐ichi

doi:10.3390/nano12183203

Cited by 3 publications

(2 citation statements)

References 63 publications

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“…Further analysis of the sample containing 15 drops of milk (see supplementary information, figure S2) looking at the peak of the DToFs from several regions of interest it could be ascertained that for a vertical distance of ∼25.8 mm the light path obtained from the peak positions corresponded to ∼46.8 mm free space. Taking into account that the refractive indices of milk and water are similar [39,40], e.g. assuming a refractive index for the sample of 1.34, then the distance reduces to ∼35 mm.…”

Section: Addition Of Milk To Water (Increase Of the Degree Of Scatter...mentioning

confidence: 99%

Potential for remote TD-NIRS imaging using a TCSPC camera

Hungerford

Αθανασόπουλος

Hirsch

et al. 2023

Meas. Sci. Technol.

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Advances in the CMOS process enables single-photon detection and timing to occur within an individual pixel. Pixel arrays incorporating this in-pixel timing methodology have been increasing in size, facilitating time of flight (ToF) imaging. This means that a fully parallel approach can be made to the collection of images, dramatically speeding up data collection in relation to sample scanning and allowing imaging based on Time-Correlated Single-Photon Counting (TCSPC) to be made in real time. In this work we make use of a commercial TCSPC camera, based on an array of 192 x 126 imaging pixels with in-pixel timing applied in the area of time-domain near infrared spectroscopy. The potential of remote imaging using this methodology was demonstrated using two model systems. The effect of increasing scattering coefficient using a liquid based (milk added to water) system to assess real time potential was investigated. Additionally a more complex system consisting of a solid with liquid interior (egg) to verify the potential of remote imaging into the interior of the object with different illumination conditions was studied. Using a time-slicing approach intensity images related to each time bin in the ToF distribution were obtained and contrast between the yolk and albumin within the egg was observed.

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Section: Addition Of Milk To Water (Increase Of the Degree Of Scatter...mentioning

confidence: 99%

Potential for remote TD-NIRS imaging using a TCSPC camera

Hungerford

Αθανασόπουλος

Hirsch

et al. 2023

Meas. Sci. Technol.

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“…Measurements of the velocity field is fundamental in understanding the behavior of flowing systems both natural, ranging from molecular biology (Bos et al, 2021;Faez et al, 2015;Huang and Choma, 2015;Wang et al, 2018) and cellular biology (Marin et al, 2017;Savorana et al, 2022;Wang et al, 2020) to physiology (Oeler et al, 2021;Sampath et al, 2018;Zhang et al, 2022) and the environment, (Aksamit and Pomeroy, 2016;Gaudin et al, 2014;Mujtaba and de Lima, 2018;Tagliavini et al, 2022;Tauro et al, 2019) and engineered, spanning biochemical reactors (Devasenathipathy et al, 2002;Hofmann et al, 2022;Kováts et al, 2018;Romano et al, 2023Romano et al, , 2021 and fundamental fluid dynamics (Humphrey et al, 1974;Kasagi and Nishino, 1991;Kim et al, 2016) to manufacturing (Eschner et al, 2019;Fischer et al, 2022) and civil (Bautista-Capetillo et al, 2014;Fu et al, 2015;Zhao et al, 2020) industry. The imaging system must fulfill resolution requirements relative to the physical system, however, no constraint is imposed on the overall scale of the physical system, PTV being applicable to nano, (Bos et al, 2021;Faez et al, 2015;Matsuura et al, 2018;Satake, 2022;Wang et al, 2018) micro, (Choi et al, 2012;Dehnavi et al, 2020;Devasenathipathy et al, 2002;Fan et al, 2010;Huang ...…”

Section: Introductionmentioning

confidence: 99%

Single-camera PTV within interfacially sheared drops in microgravity

McMackin,

Adam,

Riley

et al. 2023

Exp Fluids

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Development of experimental methods for in situ particle tracking velocimetry (PTV) is fundamental for allowing measurement of moving systems nontailored for velocimetry. This investigation focuses on the development of a post-processing methodology for single-camera PTV, without laser-sheet illumination, tracking native air-bubbles as tracer particles within a liquid drop of human insulin in microgravity. This PTV scenario was facilitated by microgravity technology known as the ring-sheared drop (RSD), aboard the International Space Station, which produced an optical imaging scenario and fluid flow geometry suitable as a testbed for PTV research. The post processing methodology performed included five steps: (i) physical system characterization and native air-bubble tracer identification, (ii) image projection and single-camera calibration, (iii) depth determination and 3D particle position determination, (iv) ray tracing and refraction correction, and (v) particle history and dataIn situ PTV in sheared drops expansion for suboptimal particles. Overall, this post processing methodology successfully allowed for a total of 1,085 particle measurements in a scenario where none were previously possible. Such post processing methodologies have promise for application to other in situ PTV scenarios allowing better understanding of physical systems whose flow is difficult to measure and or where PTV-specific optical elements (such as laser lights sheets and dual camera setups) are not permissible due to physical or safety constraints.

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Single-camera PTV within interfacially sheared drops in microgravity

McMackin

Adam

Riley

et al. 2023

Preprint

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Development of experimental methods for in situ particle tracking velocimetry (PTV) is fundamental for allowing measurement of moving systems non-tailored for velocimetry. This investigation focuses on the development of a post-processing methodology for single-camera PTV, without laser-sheet illumination, tracking native air-bubbles as tracer particles within a liquid drop of human insulin in microgravity. This PTV scenario was facilitated by microgravity technology known as the ring-sheared drop (RSD), aboard the International Space Station, which produced an optical imaging scenario and fluid flow geometry suitable as a testbed for PTV research. The post processing methodology performed included five steps: (i) physical system characterization and native air-bubble tracer identification, (ii) image projection and single-camera calibration, (iii) depth determination and 3D particle position determination, (iv) ray tracing and refraction correction, and (v) particle history and data expansion for suboptimal particles. Overall, this post processing methodology successfully allowed for a total of 1,085 particle measurements in a scenario where none were previously possible. Such post processing methodologies have promise for application to other in situ PTV scenarios allowing better understanding of physical systems whose flow is difficult to measure and or where PTV-specific optical elements (such as laser lights sheets and dual camera setups) are not permissible due to physical or safety constraints.

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Micro- and Nanoscale Imaging of Fluids in Water Using Refractive-Index-Matched Materials

Cited by 3 publications

References 63 publications

Potential for remote TD-NIRS imaging using a TCSPC camera

Potential for remote TD-NIRS imaging using a TCSPC camera

Single-camera PTV within interfacially sheared drops in microgravity

Single-camera PTV within interfacially sheared drops in microgravity

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